Occurrence of Ehrlichia canis and Hepatozoon canis and probable exposure to Rickettsia amblyommatis in dogs and cats in Natal, RN

Lopes, Maria Luiza Diniz de Sousa; Krawczak, Felipe da Silva; Lima, Júlia Teresa Ribeiro de; Fournier, Gislene Fátima da Silva Rocha; Acosta, Igor da Cunha Lima; Ramírez, Diego García; Marcili, Arlei; Labruna, Marcelo Bahia; Gennari, Solange Maria

doi:10.1590/s1984-296120180065

Cited by 12 publications

(12 citation statements)

References 44 publications

(47 reference statements)

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“…As similar examples, in the last decade, novel strains of tick-borne agents of felids have been identified in Brazil [ 60 ] and in Europe [ 61 , 62 ]. Arthropod-borne diseases are highly enzootic and circulate between domestic and wild felids of Brazil and Europe, where they can also be shared with canids living in sympatry [ 60 , 61 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 ]. Therefore, further studies are necessary to investigate any genetic variability in A. abstrusus isolates which infect domestic cats and wildlife living in Amazonia and Brazil and if, as for other metastrongylid nematodes and arthropod-borne pathogens, there is a potential impact on their genetic make-up by bridging infections between different animal species.…”

Section: Discussionmentioning

confidence: 99%

First Case of Clinical Cat Aelurostrongylosis in the Brazilian Amazon: Clinical and Molecular Insights

et al. 2021

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Aelurostrongylus abstrusus is the most important respiratory parasite infecting domestic cats worldwide. Nevertheless, most records and epizootiological data come from Europe, whilst poor and fragmentary information are available for other regions, including the Americas. The present article describes the first description of cat aelurostrongylosis from Amazonia, Brazil. Eighty-one cats, 13 from a shelter and 68 admitted at the Teaching and Research Unit in Veterinary Medicine (UV) at the Federal University of Acre (UFAC), Brazil, were included in the study. For all cats, three faecal samples from consecutive defecations were examined using the Baermann’s technique. Nematode first stage larvae (L1), retrieved in 2/81 (2.5%) samples, were microscopically identified as A. abstrusus and then subjected to a molecular assay able to identify the three most important species of metastrongyloids infecting felids. This test confirmed the A. abstrusus identity in one sample, while the second scored negative. The cat with confirmed aelurostrongylosis showed radiographic changes, i.e., an interstitial pattern, compatible with the infection. The other cat, which scored positive at the Baermann’s examination, was apparently healthy at the physical examination and showed no thoracic alterations. The occurrence of A. abstrusus in domestic cats from Brazilian Amazon is herein demonstrated for the first time. Clinical, epizootiological and molecular implications are discussed.

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Section: Discussionmentioning

confidence: 99%

First Case of Clinical Cat Aelurostrongylosis in the Brazilian Amazon: Clinical and Molecular Insights

et al. 2021

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“…Epidemiological studies on the etiologic agents of CME and CCT have already been carried out in several regions of Brazil (LEAL et al, 2015;MAKINO et al, 2016;SOARES et al, 2017;VIEIRA et al, 2018;LOPES et al, 2019). However, there are few studies on the molecular epidemiology of these agents in the northern region of the country.…”

Section: Discussionmentioning

confidence: 99%

Molecular detection of Ehrlichia canis and Anaplasma platys in dogs from municipality of Belém, State of Pará, Brazil

et al. 2019

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The occurrence of diseases transmitted by ticks in dogs is very frequent in Brazil, among these diseases we can highlight the ehrlichiosis and anaplasmosis, which are caused by Ehrlichia canis and Anaplasma platys, respectively. The objective of this study was to survey the occurrence of these pathogens in blood samples from domiciled and stray dogs from the city of Belém, Pará. Two hundred and seventy six dogs were sampled for convenience, and the DNA extracted from the blood of these animals was submitted to nested-PCR for research of E. canis and A. platys. E. canis DNA was detected in 39.4% (109/276) and A. platys DNA in 23.1% (64/276) of the samples, there was a statistically significant difference between the frequency of these agents (P<0.0001), and there was coinfection in 13.4% (37/276) of animals. The frequency of detection of these parasites was higher in stray dogs than in those domiciled for both E. canis (OR=2.84) and A. platys (OR=10.5). Considering the results, it was possible to conclude that E. canis and A. platys are present in the studied population, with stray dogs being more affected by these parasites.

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“…A positive control, from a dog known to be positive for each pathogen tested, and a negative control consisting of water were included in each technique performed, which are described below. Positive control canine DNA samples consisted of E. canis-infected blood from the study of Moraes-Filho et al (2015), H. canis-infected spleen from the study of Lopes et al (2019) The real-time PCR for E. canis was used to amplify a 378 base pair (bp) fragment of the dsb gene encoding a disulfide-forming protein, using the Dsb321 and Dsb671 primers and a species-specific probe, as previously described by Doyle et al (2005). To detect B. vogeli, a 90 bp fragment of the hsp70 gene was amplified using the B.c hsp70-F and B.c hsp70-R primers and a species-specific probe, in accordance with the conditions described by Peleg et al (2010).…”

Section: Collection and Identification Of Ectoparasitesmentioning

confidence: 99%

Occurrence of tick-borne pathogens in dogs in a coastal region of the state of Ceará, northeastern Brazil

Fonsêca

Oliveira

Jorge

et al. 2022

Rev. Bras. Parasitol. Vet.

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The aim of this study was to determine the occurrence of tick-borne pathogens (Ehrlichia canis, Babesia vogeli, Hepatozoon spp. and Rickettsia spp.) in dogs in Vila de Jericoacoara, coastal region of Ceará, Brazil. Blood samples were collected from 153 animals and analyzed using molecular and serological methods. Sixty animals were found to be infected or exposed to at least one of the pathogens studied. Babesia vogeli was the most prevalent pathogen (15%), followed by E. canis (13.7%) and Hepatozoon spp. (11.8%), which was identified as Hepatozoon canis through sequencing. Twenty dogs (13%) were seroreactive to Rickettsia spp. Rhipicephalus sanguineus sensu lato was observed on 11.8% of the animals. There were associations between age (< 3 years old) and positivity for B. vogeli, and between habitation (stray dogs) and positivity for H. canis. There were also associations between anemia and infection with H. canis, and between leukopenia and exposure to Rickettsia spp. No association was detected between clinical alterations and infection with or exposure to the pathogens studied. The results confirmed that pathogens of veterinary importance are circulating in northeastern Brazil and showed that dogs are exposed to Rickettsia species with zoonotic potential, thus indicating a need for vector control measures.

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Occurrence of Ehrlichia canis and Hepatozoon canis and probable exposure to Rickettsia amblyommatis in dogs and cats in Natal, RN

Cited by 12 publications

References 44 publications

First Case of Clinical Cat Aelurostrongylosis in the Brazilian Amazon: Clinical and Molecular Insights

First Case of Clinical Cat Aelurostrongylosis in the Brazilian Amazon: Clinical and Molecular Insights

Molecular detection of Ehrlichia canis and Anaplasma platys in dogs from municipality of Belém, State of Pará, Brazil

Occurrence of tick-borne pathogens in dogs in a coastal region of the state of Ceará, northeastern Brazil

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