Occurrence of GalNAcβ1-4GlcNAc Unit inN-Glycan of Royal Jelly Glycoprotein

First Evidence for Occurrence of Galβ1-3GlcNAcβ1-4Man Unit inN-Glycans of Insect Glycoprotein: β1-3Gal and β1-4GlcNAc Transferases Are Involved inN…

“…In our previous papers, 8,9) we reported that, among the total N-glycans of royal jelly glycoproteins, no antigenic N-glycan bearing the Fuca1-3GlcNAcb1-Asn unit occurs, but the typical high-mannose type structure (Man9-4GlcNAc2) accounts for about 72z of total N-glycan, a biantennary-type structure (GlcNAc2Man3GlcNAc2) about 8z, a hybrid-type structure (GlcNAc1Man4GlcNAc2) about 3z, and a GalNAc-containing structure (GalNAc1GlcNAc2Man3GlcNAc2) about 3z. In a detailed structural analysis of the remaining minor components of royal jelly N-glycans, we found that at least three N-glycans had an b-linkage galactose residue.…”

“…Liberated oligosaccharides were N-acetylated, desalted, and labeled with 2-aminopyridine as described in our previous reports. 8,9) Three N-glycans containing a galactosyl residue (C6, C7, and D3) were puriˆed from the total pyridylamino (PA) derivatives by reverse-phase (RP) HPLC using a Shiseido Capcell pak C18 MG column (Shiseido, 10×250 mm) ( Fig. 1-[A]) and size fractionation (SF) HPLC using a Shodex Asahipak NH2P-50 column (Showa Denko, 10×250 mm) ( Fig.…”

“…1-[B]-I and -II)) as described in our previous report. 8,9) The ion-spray mass (IS-MS) analysis of these PA-sugar chains showed that C6 is HexNAc2Hex-5HexNAc2-PA (m W z 860. 2＋ ), respectively.…”

Honey and royal jelly, like human milk, abrogate lectin-dependent infection-preceding Pseudomonas aeruginosa adhesion

“…RJ, which is produced by young nurse bees from a honey and flower pollen mixture with secretions from their cephalic salivary glands (Moritz and Southwick, 1992), differs from honey in its higher water content (60-70%), 10-16% carbohydrates (some of honey origin) and much higher protein levels (around 14%), most of them gps (Kimura et al, 2000(Kimura et al, , 2002Scarselli et al, 2005), and 3-7% lipids as well as richer content of other vital components (Takenaka, 1982;Schmitzova et al, 1998;Albert et al, 1999;Stocker et al, 2005). The most prominent 'major' RJ proteins, 'MRJPs' 1-5, ranging from 49 up to 87 kDa and also named apalbumins (Albert and Klaudiny, 2004), constitute around 90% of the total RJ proteins (Schmitzova et al, 1998;Sano et al, 2004;Santos et al, 2005;Scarselli et al, 2005).…”

Identification of a Royal Jelly Glycoprotein That Carries Unique Complex-Type N-Glycans Harboring the T-Antigen (Galβ1-3GalNAc) Unit

“…The detailed structures of these typical high-mannose-type and some complex-type N-glycans are described in our previous reports. 1,6,7) In this study, we identified one royal jelly glycoprotein, MRJP 1, carrying unique N-glycans harboring the RJGPs. 1, RP-HPLC profile of total tryptic peptides froMRJPs.…”