Occurrence of genes associated with virulence in Escherichia coli isolates from chicken carcasses at different stages of processing at a slaughterhouse

Pinheiro, João Juliano; Gazal, Luiz Eduardo de Souza; Araujo, Gabriella Oliveira de; Brito, Kelly Cristina Tagliari de; Tazzo, Ivonete Fátima; Mesquita, Rafael Tonini; Kobayashi, Renata Katsuko Takayama; Otutumi, Luciana Kazue; Beltrami, José Matheus; Cavalli, Lissandra Souto; Brito, Benito Guimarães de

doi:10.1007/s42770-021-00549-5

Cited by 1 publication

(2 citation statements)

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“…In this study, the detection rates of 12 VFs varied from 1.7% to 100%. Interestingly, similar to our results, Johnson et al reported 13 , 56 that the detection rates of VFs varied from 0.4% to 98%. and the high-risk epidemic ST131 isolates carried more virulence genes in comparison to the non-ST131 isolates, especially iha, hlyD, sat, iutA, fyuA, malX, ompT and traT, tsh and espC .…”

Section: Discussionsupporting

confidence: 92%

“…It was reported that the virulence genes appeared more frequently in carbapenemase producers than in the non-producers, and fimA, iutA and cnf1 exhibited significant differences. 13,56 In this study, we found that hek/hra, iroN, iucC, kpsM, and papGII-III showed statistical differences among bla CTX-M subtypes. This finding suggested that there might be a possible relationship between the bacterial virulence and resistance.…”

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confidence: 54%

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The Characteristics of Extended-Spectrum β-Lactamases (ESBLs)-Producing Escherichia coli in Bloodstream Infection

Li¹,

Xu²,

Tang³

et al. 2023

IDR

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Background Bloodstream infection (BSI) is a common type of infection frequently diagnosed in clinics. The emergence and spread of ESBLs-producing Escherichia coli ( E. coli ) has emerged as one of the biggest challenges in global community health. Methods The production of ESBLs was determined by the composite disk diffusion method. The expression of the various resistance and virulence genes were detected by PCR and sequencing. Multi-locus sequence typing (MLST) and phylogenetic groups were used for the classification. The transfer of resistant plasmids was determined by conjugation assay. The statistical differences were analyzed using Statistical Product and Service Solutions (SPSS) version 23.0. Results A total of 60 strains of ESBLs-producing E. coli were collected. The resistance genes that were identified included bla CTX-M , bla TEM , bla SHV , bla OXA-1 and mcr-1 . The most common one was the bla CTX-M including bla CTX-M-27 (n = 16), bla CTX-M-14 (n = 15), bla CTX-M-15 (n = 11), bla CTX-M-55 (n = 14) and bla CTX-M-65 (n = 5). A total of 31 STs were detected, and the most abundant among which was ST131 (n = 16, 26.7%). Most of the E. coli (n = 46, 76.7%) belonged to the groups B2 and D. And some virulence genes were related to the classification of the E. coli . Among them, the detection rates of hek/hra, kpsMII and papGII-III in groups B2 and D were higher than those in groups A and B1. The detection rates of cnf1, iucC and papGII-III in ST131 were higher than those in non-ST131. And the distributions of hek/hra, iroN, iucC, kpsMII and papGII-III were related to the bla CTX-M subtypes. Finally, most bacterial (n = 32, 53.3%) resistance genes could be transferred between the bacteria by plasmids, especially IncFIB. Conclusion ESBLs-producing E. coli in BSI exhibited had high resistance rates and carried a variety of virulence factors (VFs). This is necessary to strengthen the monitoring of ESBLs-producing isolates in the medical environment.

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Section: Discussionsupporting

confidence: 92%

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confidence: 54%