Occurrence of Glycogen in Inclusions of the Psittacosis-Lymphogranuloma Venereum-Trachoma Agents

Gordon, Francis B.; Quan, Alice L.

doi:10.1093/infdis/115.2.186

Cited by 104 publications

(47 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Early methods relied on presumed tissue or host affinities, presumed unique epidemiology, or on specific serology, all characteristics of host response to chlamydiae. In the mid-l960s, phenotypic traits of specific chlamydial strains were identified : glycogen accumulation and sulfadiazine sensitivity (Gordon & Quan, 1965;Lin & Moulder, 1966). These characteristics and inclusion morphology became the basis for the description and differentiation of C. trachomatis and C. psittaci species (Page, 1968 (Cox et al, 1988;Fukushi & Hirai, 1989Grayston et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

Identification of nine species of the Chlamydiaceae using PCR-RFLP

Everett¹,

Andersen

1999

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

The family Chlamydiaceae contains two genera and nine species. Rapid and easy identification of these species is essential for taxonomic, epidemiological and clinical determinations. Currently, DNA sequence analysis is the only accepted method that decisively distinguishes all nine species. In this study, a simple and rapid PCR-RFLP procedure was developed b y which laboratorycultured chlamydial specimens could be identified. To accomplish this, conserved oligonucleotide primers and restriction sites were deduced from 165 and 235 rRNA sequence data from >50 chlamydial strains representing all nine species. DNA from 25 previously characterized chlamydial strains were tested with these primers and restriction enzymes. All nine chlamydial species were reliably distinguished in the tests. The procedure was optimized by adjusting the annealing temperature using both a standard and a heat-activated DNA polymerase to reduce mismatch PCR amplification of mycoplasmas and other bacteria. The result was that a PCR method for species identification of chlamydial isolates and for distinguishing mycoplasmas and chlamydiae was created. This method can be used to rapidly identify known species of the f am i I y Chlamydiaceae .

show abstract

Section: Introductionmentioning

confidence: 99%

Identification of nine species of the Chlamydiaceae using PCR-RFLP

Everett¹,

Andersen

1999

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

show abstract

“…Within the genus Chlamydia an important species characteristic is the presence or absence in the inclusion of a carbohydrate which is probably glycogen (Gordon & Quan, 1965). The role of this material in the multiplication of the organism has not been elucidated, nor is it certain whether it is produced by the organism or the host cell (see Moulder, 1964).…”

Section: Introductionmentioning

confidence: 99%

The development of TRIC organisms in cell cultures during multiple infection

Evans

1972

J. Hyg.

View full text Add to dashboard Cite

SUMMARYThe relationship between multiplicity of infection and the yield of organisms and of polysaccharide was studied in BHK 21 cells infected with TRIC organisms. Although an increase in the multiplicity of infection resulted in an increase in the absolute number of organisms per culture, there was a decrease in relation to the number of infecting organisms. The amount of polysaccharide produced was independent of multiplicity of infection; it was not limited by the concentration of glucose in the medium. Polysaccharide leaked from cells and was slowly broken down in the culture medium.

show abstract

“…As determined by Giemsa staining of cultured cells. As evidenced by positive iodine staining of inclusions (5).…”

mentioning

confidence: 99%