Occurrence of Grapevine Leafroll-Associated Viruses in China

Liu, M.-H.; Li, M.-J.; Qi, Hai-Long; Guo, Ran; Liu, X.-M.; Wang, Q.; Cheng, Yuqin

doi:10.1094/pdis-01-13-0048-re

Cited by 20 publications

(24 citation statements)

References 27 publications

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“…In this study, PCR and nPCR were used to detect GLRaV-1 in 134 grapevine samples, and the results showed that nPCR can detect more GLRaV-1 isolates than conventional PCR. The overall incidence of GLRaV-1 was 36.4 % (48/132), which was far higher than what was reported previously [14]. Thus, the use of RT-nPCR can largely increase the detection sensitivity, and this method could be used as an alternative method when no real-time PCR device is available.…”

Section: Discussionmentioning

confidence: 69%

“…[1] and one of the main causative agents of GLD in other countries [6,9,15,17,22]. Recently, a survey in China showed that GLD was widespread and that GLRaV-1 was the most common agent after GLRaV-3 [14]. Using untested grapevine materials for propagating or grafting increases the risk of spreading GLD; therefore, efficient detection of GLRaV-1 and -3 is urgently required for certification.…”

Section: Introductionmentioning

confidence: 99%

“…However, conventional reverse transcription PCR (RT-PCR) detection of most grapevine viruses still has two unsolved problems: primers are not available for all virus variants, and detection sensitivity is low. GLRaV-1 has been detected at a lower rate (24.9 %) than GLRaV-3 (100 %) in grapevine samples exhibiting GLD in China [14]. However, sequence variations of GLRaV-1 isolates from China remain unknown, which prevents improvement of nucleic-acid-based detection methods.…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity and recombination analysis of grapevine leafroll-associated virus 1 from China

et al. 2015

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Grapevine leafroll-associated virus 1 (GLRaV-1) is one of the causal agents of grapevine leafroll disease (GLD). To investigate the prevalence and genetic variation of GLRaV-1 in China, 132 grapevine samples from 14 Chinese provinces and regions were tested using reverse transcription PCR (RT-PCR) and reverse transcription nested PCR (RT-nPCR). The samples included symptomatic and asymptomatic cultivars, and 36.4% of them tested positive for GLRaV-1. 'Beida' samples, previously identified as virus-free rootstocks, were also found to be infected with GLRaV-1 with an incidence of 40 . GLRaV-1 coat protein (CP) genes and heat-shock protein 70 (HSP70) genes from 43 GLRaV-1 isolates were selected and sequenced. Phylogenetic analysis of global CP and HSP70 gene sequences showed that all variants belonged to eight and seven groups, respectively. For CP gene sequence variants, group 4 was a new group that included only Chinese isolates. The results also showed that natural selection, rather than random processes, led to the evolution of variants belonging to CP gene sequence variants in group 2 and group 8. Furthermore, three new recombination events were identified in the GLRaV-1 CP gene population. This is the first report on the genetic variation of GLRaV-1 isolates in China, and this study will benefit grape clean-plant programs in China.

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Section: Discussionmentioning

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic diversity and recombination analysis of grapevine leafroll-associated virus 1 from China

et al. 2015

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“…In many grape-growing countries and regions, virus surveys have been conducted on grapevines, for example, in Argentina (Volpe et al, 2010), Chile (Fiore et al, 2011), China (Liu et al, 2013), the United States (Jones et al, 2015), Slovakia, the Czech Republic (Glasa et al, 2015) and in two regions of northeastern Brazil (Catarino et al, 2015), in which the incidence of viruses was highly variable, depending on the viral species, grapevine cultivars and …”

Section: Incidence Of Viruses and Viroid In Grapevinesmentioning

confidence: 99%

Molecular characterization of GSyV-1 and GLRaV-3 and prevalence of grapevine viruses in a grape-growing area

Moura

Fajardo

Eiras

et al. 2018

Sci. agric. (Piracicaba, Braz.)

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ABSTRACT:The aims of this study were to determine the prevalence of viruses in 119 samples from 32 grapevine cultivars, collected from nine vineyards in a specific grape-growing area in southeastern Brazil, perform a partial molecular characterization of 14 isolates of Grapevine Syrah virus 1 (GSyV-1) and Grapevine leafroll-associated virus 3 (GLRaV-3) and assess the coat protein genetic variability of these viruses. The detection of viruses was implemented by realtime RT-PCR (reverse transcription polymerase chain reaction) aiming to detect seven viruses and one viroid. With the exception of the Grapevine Cabernet Sauvignon reovirus (GCSV), the viruses and viroid that were evaluated were widespread in the sampled areas, often in high prevalence and multiple infections, ranging from 15 % up to 76 %. Eight isolates of GSyV-1 and six of GLRaV-3, partially characterized by complete coat protein gene nucleotide sequencing and a variability study showed nucleotide identities ranging from 91 % to 99 % (GSyV-1) and from 98 % to 100 % (GLRaV-3) among themselves, respectively. Comparisons between conventional and real-time RT-PCR detections were implemented for GSyV-1 and GLRaV-3 infections. Analysis of genetic variability indicated molecular differences between GSyV-1 and GLRaV-3 isolates and negative selection acting on the coat protein gene of both viruses. This is the first report of GSyV-1 in commercial vineyards in Brazil. The survey revealed widespread infections of seven important pathogens in one prominent Brazilian grape-producing region implying contaminated grapevine cuttings in the spread of disease.

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“…Recently, a survey showed that GLD is widespread in China, and GLRaV‐2 was identified as one of the main agents in grapevines showing GLD (Liu et al. ). However, surveys of GLRaV‐2 in symptomless grapevine samples, especially in rootstocks, are limited.…”

Section: Introductionmentioning

confidence: 99%

Detection and Sequence Analysis of Grapevine Leafroll‐Associated Virus 2 Isolates from China

Fan

Dong

Zhang

et al. 2015

Journal of Phytopathology

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Several grapevine leafroll‐associated viruses (GLRaVs) have been found frequently in grapevines behaving GLD. Among them, GLRaV‐2 is the only one belonging to Closterovirus, and mainly induces leafroll symptoms and graft incompatibility. In this study, new degenerate primer pairs designed against the HSP70 gene were applied in polymerase chain reaction (PCR) and nested PCR (nPCR) to detect GLRaV‐2 in 132 samples collected from 14 provinces and regions of China. Of the samples, 51.5% were infected with GLRaV‐2, and most did not exhibit GLD symptoms. Some popular grape cultivars had a high incidence of GLRaV‐2 infection, such as Cabernet Sauvignon (92.3%), Chardonnay (80%), Red Globe (75%) and Italian Riesling (73.7%). ‘Beta’ rootstocks, previously identified as negative samples, were also found to be highly infected with GLRaV‐2 (50%). GLRaV‐2 isolates obtained in this study showed identities ranging from 68.9% to 100% and 76.47% to 100.0% at the nucleotide and amino acid levels, respectively. Phylogenetic analysis based on the HSP70 gene showed that all GLRaV‐2 isolates in China belong to three of five reported phylogenetic groups. Different variants belonging to the PN and RG groups were present in a single isolate. The results showed that the new degenerate primer pairs could detect more GLRaV‐2 isolates than the previously reported primers. This is the first detailed report on the prevalence and gene diversity of GLRaV‐2 in China and also provides an nPCR method to improve the sensitivity of PCR as an alternative method when no real‐time PCR device is available.

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Occurrence of Grapevine Leafroll-Associated Viruses in China

Cited by 20 publications

References 27 publications

Genetic diversity and recombination analysis of grapevine leafroll-associated virus 1 from China

Genetic diversity and recombination analysis of grapevine leafroll-associated virus 1 from China

Molecular characterization of GSyV-1 and GLRaV-3 and prevalence of grapevine viruses in a grape-growing area

Detection and Sequence Analysis of Grapevine Leafroll‐Associated Virus 2 Isolates from China

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