OCCURRENCE OF H,‐ AND H2‐HISTAMINE RECEPTORS IN THE GUINEA‐PIG GALL BLADDER in situ

Impicciatore, M

doi:10.1111/j.1476-5381.1978.tb17292.x

Cited by 23 publications

(11 citation statements)

References 8 publications

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“…In strips of human gallbladder it was found that histamine contracted the tissue via the Hi-receptor [18]. Others [19][20][21], have demonstrated that the net effect of exogenous histamine on guinea-pig gallbladder strips is one of contraction via Hi-receptor stimulation but this hides a relaxatory component mediated by the Hz-receptor. To date, the possible involvement of the histamine H3-receptor in this organ has not been investigated.…”

Section: Inflamm Resmentioning

confidence: 98%

The source and action of histamine in the isolated guinea-pig gallbladder

et al. 1995

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We have investigated the effects of histamine on motility of the gallbladder and characterized the receptor types involved. Histamine and the histamine H1-receptor agonist, 2-thiazolylethylamine (2-TEA) contracted the isolated guinea-pig gallbladder strip in a dose dependent manner. The contractile response to histamine was shifted to the right by the H1-receptor antagonist, mepyramine. In pre-contracted gallbladder strips, the H2-receptor agonist dimaprit reduced the tension generated in a dose dependent fashion. The histamine H2-receptor antagonist, ranitidine shifted the histamine concentration effect curve to the left and attenuated the dose dependent relaxations elicited at high concentrations. The histamine H3-receptor agonist, (R)-alpha-methylhistamine (RMHA) elicited dose dependent contraction of the tissue which was significantly inhibited in the presence of mepyramine. The effects of electrical field stimulation (EFS) on the strips were not significantly altered by the presence of RMHA (10(-10) - 10(-7) M) indicating little pre-synaptic H3 activity in this tissue. Histamine immunoreactivity (IR) was detected in gallbladder whole mount preparations of the mucosa and the muscularis/serosa. The histamine IR appeared cell bound in cells of varying morphological characteristics but no IR was detected in nerve fibres or cell bodies (ganglia). Alcian blue staining was consistent with the distribution of histamine IR cells as mast cells. The results indicate that histamine is distributed in the guinea-pig gallbladder and it can regulate contractile activity via activation of H1 and H2 but not H3 receptors.

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Section: Inflamm Resmentioning

confidence: 98%

The source and action of histamine in the isolated guinea-pig gallbladder

et al. 1995

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“…The presence of excitatory muscarinic cholinoceptors (Davison & F6sel, 1975) and of HI and H2-histamine receptors, which mediate contraction and relaxation, respectively (Impicciatore, 1978). have been demonstrated clearly.…”

Section: Introductionmentioning

confidence: 99%

The OCCURRENCE OF POSTSYNAPTIC Α‐ AND Β‐ADRENOCEPTORS IN THE GUINEA‐PIG GALL BLADDER

Doggrell

Scott

1980

British J Pharmacology

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I Guinea-pig gall bladder strips were contracted by (--noradrenaline, 10-5 M, and by field stimulation at 5 Hz (in the absence or presence of 10-6 M atropine) and relaxed to 10-s M (--isoprenaline. (-)-Adrenaline, 10-5 M, predominantly contracted, but sometimes relaxed, this preparation. 2 In the presence of 10-6 M phentolamine, contractions to (--noradrenaline and to (-)-adrenaline were reversed to relaxations. The relaxations produced by (-)-isoprenaline were unaltered. In the presence of 10'6 M propranolol, contractions to (-)-noradrenaline increased in magnitude, relaxations to (-)-adrenaline were reversed to contractions, and relaxations to (-)-isoprenaline were abolished. These results demonstrate the presence of postsynaptic ax-adrenoceptors which mediate contractions, and postsynaptic ,B-adrenoceptors which initiate relaxations, in the guinea-pig gall bladder. 3 The contractile responses to continuous field stimulation for 5 min at 5 Hz in Krebs solution alone were reduced in magnitude by propranolol, 10-6 M. In the presence of 10-6 M atropine (added to eliminate the cholinergic component of the response), propranolol, 1lo' M, had no effect on responses to stimulation at 5 Hz. Thus propranolol reduced the response to cholinergic stimulation in this tissue; the basis of this effect is unclear. In the absence or presence or atropine (10-6 M), the responses to 5 Hz were smaller in magnitude in the presence than absence of phentolamine, 10-6 M. This suggests that the responses to field stimulation of the guinea-pig gall bladder may, in part, be due to the release of endogenous noradrenaline which acts at postsynaptic a-adrenoceptors.

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“…The H2 recep tor antagonist cimetidine did not influence the effects of oxytocin, but potentiated the relaxation caused by 8-/-lysine-vasopressin in guinea pigs. This effect of cimetidine ex cludes the possibility that 8-/-Iysine-vasopressin acts via Hi histamine receptors, known as receptors mediating a relaxation in smooth muscle preparations [8], since their blockade did not inhibit, but potentiated re laxation caused by 8-/-lysine-vasopressin. Anyway, an explanation of such a cimetidine effect demands further experiments.…”

Section: Discussionmentioning

confidence: 96%

Relaxant Effects of Oxytocin and 8-l-Lysine-Vasopressin on Guinea Pig and Human Gallbladder Strips in vitro Contracted by Histamine

Janković

Beleslin²

1991

Digestion

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Both human and guinea pig gallbladder strips were tonically contracted by supramaximal concentrations of histamine in vitro. Guinea pig gallbladder strips were also tonically contracted by potassium, with no spontaneous relaxation after 5 min. The amplitude of the contractions caused by histamine decreased spontaneously only by 34 ± (SD) 10% after 5 min in guinea pig strips and by 25 ± 7% after 5 min in human strips. Both oxytocin and 8-l-lysine-vasopressin dose-dependently relaxed almost completely contractions caused by histamine and potassium in guinea pigs. In humans, only 8-l-lysine-vasopres-sin decreased the amplitude of histamine contractions. In both species, neither oxytocin nor 8-l-lysine-vasopressin affected the basal tone of strips.

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OCCURRENCE OF H,‐ AND H₂‐HISTAMINE RECEPTORS IN THE GUINEA‐PIG GALL BLADDER in situ

Cited by 23 publications

References 8 publications

The source and action of histamine in the isolated guinea-pig gallbladder

The source and action of histamine in the isolated guinea-pig gallbladder

The OCCURRENCE OF POSTSYNAPTIC Α‐ AND Β‐ADRENOCEPTORS IN THE GUINEA‐PIG GALL BLADDER

Relaxant Effects of Oxytocin and 8-<i>l</i>-Lysine-Vasopressin on Guinea Pig and Human Gallbladder Strips in vitro Contracted by Histamine

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