Occurrence of Hookworm and the First Molecular and Morphometric Identification of Uncinaria stenocephala in Dogs in Central Europe

Štrkolcová, Gabriela; Mravcová, Kristína; Mucha, Rastislav; Mulinge, Erastus; Schreiberová, Andrea

doi:10.1007/s11686-021-00509-x

Cited by 9 publications

(2 citation statements)

References 52 publications

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“…Infection occurs via free-living larvae or paratenic hosts (e. g. mice). If humans become infected, it can lead to the cutaneous larva migrans ( Bowman et al, 2010 ; Štrkolcová et al, 2022 ). The infection of the studied raccoon dogs was probably caused to a large extent by eating infected mice, which could have been species such as Microtus arvalis which could be detected in this study.…”

Section: Discussionmentioning

confidence: 99%

The hidden threat: Exploring the parasite burden and feeding habits of invasive raccoon dogs (Nyctereutes procyonoides) in central Europe

Schantz,

Dörge,

Peter

et al. 2023

International Journal for Parasitology: Parasites and Wildlife

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Section: Discussionmentioning

confidence: 99%

The hidden threat: Exploring the parasite burden and feeding habits of invasive raccoon dogs (Nyctereutes procyonoides) in central Europe

Schantz,

Dörge,

Peter

et al. 2023

International Journal for Parasitology: Parasites and Wildlife

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“…and may even cause patent infections in humans [6,17]. The northern canine hookworm, Uncinaria stenocephala, is a parasite of dogs in temperate areas which can have a medium-high prevalence [18][19][20]. Whilst considered less pathogenic than Ancylsotoma caninum, this parasite can still cause clinical disease.…”

Section: Introductionmentioning

confidence: 99%

Development of In Vitro Assays with the Canine Hookworm Uncinaria stenocephala and Assessment of Natural Plant Products for Anti-Parasitic Activity

et al. 2023

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Enteric helminth infection is an increasing concern in companion animals due to reports of resistance to commonly used anthelmintic drugs. Thus, the assessment of new therapeutic options such as bioactive dietary additives is of high importance. Here, we adapted egg hatch, larval migration, and larval motility assays to screen extracts of several natural ingredients against the canine hookworm Uncinaria stenocephala, a prevalent parasite of dogs in northern Europe. Egg hatch and larval migration assays were established showing that the anthelmintic drugs levamisole and albendazole had strong anti-parasitic activity against U. stenocephala, validating the use of these assays for the assessment of novel anti-parasitic substances. Subsequently, we identified that extracts from the seaweed Saccharina latissima, but not extracts from grape seed or chicory, significantly inhibited both hatching and larval migration. Finally, we showed that α-linolenic acid, a putative anti-parasitic compound from S. latissima, also exhibited anti-parasitic activity. Collectively, our results established a platform for the screening for anthelmintic resistance or novel drug candidates against U. stenocephala and highlighted the potential use of seaweed extracts as a functional food component to help control hookworm infection in dogs.

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Evaluation of a commercial coproantigen immunoassay for the detection of Toxocara cati and Ancylostoma tubaeforme in cats and Uncinaria stenocephala in dogs

et al. 2022

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Coproantigen immunoassays (IDEXX Fecal Dx® antigen tests) were evaluated for their ability to identify Toxocara cati and Ancylostoma tubaeforme infections in cats and Uncinaria stenocephala infection in dogs. Five cats were experimentally infected with 500 embryonated eggs of T. cati, eight cats with 500 third-stage larvae (L3) of A. tubaeforme and seven dogs with 500 L3 of U. stenocephala. In addition to the three coproantigen tests, the course of infection was monitored by a combined sedimentation-flotation method with ZnSO4 as flotation medium (specific gravity: 1.28–1.30) and a modified McMaster method in case of copromicroscopically positive samples. Eggs of T. cati were first observed between 28 and 54 days post infection (dpi) in four of the five infected cats. In these four cats, positive roundworm coproantigen signals were obtained between 16 and 44 dpi. Positive coproantigen signal always preceded egg observations, but the interval varied between 6 and 30 days. Hookworm-specific positive coproantigen signals were detected in seven of the eight A. tubaeforme infected cats between 10 and 52 dpi, while consecutive egg excretion was observed in three cats between day 26 and 54 pi. Of these three, coproantigen signal preceded egg observation by 12 to 24 days. Four cats had positive coproantigen results in the absence of egg excretion, and one cat never achieved a positive result for egg or coproantigen. In six of seven U. stenocephala infected dogs, infection was confirmed by copromicroscopy between 16 and 24 dpi as well as for hookworm coproantigen between 10 and 14 dpi. Coproantigen signal was detected prior to egg observation by 2 to 14 days. No cross-reactions between the roundworm, hookworm und whipworm tests occurred in study animals. The results of this study demonstrate the ability of the coproantigen tests to detect the common roundworm and hookworm infections in cats and U. stenocephala infections in dogs as well as the ability to detect the prepatent stage of infection.

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Occurrence of Hookworm and the First Molecular and Morphometric Identification of Uncinaria stenocephala in Dogs in Central Europe

Cited by 9 publications

References 52 publications

The hidden threat: Exploring the parasite burden and feeding habits of invasive raccoon dogs (Nyctereutes procyonoides) in central Europe

The hidden threat: Exploring the parasite burden and feeding habits of invasive raccoon dogs (Nyctereutes procyonoides) in central Europe

Development of In Vitro Assays with the Canine Hookworm Uncinaria stenocephala and Assessment of Natural Plant Products for Anti-Parasitic Activity

Evaluation of a commercial coproantigen immunoassay for the detection of Toxocara cati and Ancylostoma tubaeforme in cats and Uncinaria stenocephala in dogs

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