Occurrence of human pathogenic viruses in drinking water and in its sources: A review

Panizzolo, Marco; Gea, Marta; Carraro, Elisabetta; Gilli, Giorgio; Bonetta, Silvia; Pignata, Cristina

doi:10.1016/j.jes.2022.07.035

Cited by 9 publications

(1 citation statement)

References 136 publications

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“…Groundwater, unlike surface water, is a more stable environment suffering from few changes in its physical-chemical parameters, constituting a favorable matrix for the survival of viruses [61]. Viral presence was not found in surface matrices or with a water truck, which is supplied by superficial matrices.…”

Section: Discussionmentioning

confidence: 98%

Prevalence of Enterovirus in Water Consumed in Rural Areas in a State in the Midwest Region of Brazil

et al. 2023

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Individuals in rural communities often obtain water from surface and groundwater sources, where the microbial quality is often unknown. Enteric viruses are among the main pathogenic microorganisms responsible for waterborne disease outbreaks. Thus, the objective of this work was to search for enterovirus in water samples from 25 rural communities. For this, 160 water samples were collected. Detection and quantification of the enterovirus (EV) were performed through molecular tests using the two main amplification reagents for qPCR. The prevalence of EV was identified in 4.4% (7/160) of the samples when Sybr Green® was used, all in groundwater sources. Additionally, EV was found in 9.7% of shallow tubular wells, 3.8% of deep tubular wells, 4.3% of shallow dug wells and 5.9% of spring water. When using TaqMan®, there was no amplification of the EV cDNA. Conclusions: Sybr Green®, being a more accessible reagent, has a greater predilection for molecular tests, but the study showed that Taqman® could suffer less interference from environmental samples, resulting in more reliable values of viral quantification. In this context, the detection of EV in groundwater can help in monitoring the virus in this source, in addition to helping managers of these communities in decision making.

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Section: Discussionmentioning

confidence: 98%