Occurrence of human sapoviruses in wastewater and stool samples in Rio De Janeiro, Brazil

Fioretti, Júlia Monassa; Rocha, Mônica Simões; Fumian, Túlio Machado; Ginuino, A.; Silva, T.P. da; Assis, Matheus R. de; Rodrigues, Janaína; Carvalho-Costa, Filipe Aníbal; Miagostovich, Marize Pereira

doi:10.1111/jam.13205

Cited by 30 publications

(24 citation statements)

References 42 publications

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“…Influent samples showed a 56.0% positive percentage, which is significantly lower than that in other studies carried out in Japan and the United States, where SaV was detected in almost all the influent samples (15,19,20), but higher than that in similar surveillances in the Mediterranean region (16) or in South America (18). Effluents showed a detection rate of 23.9%, which is low compared to rates in Japanese (conventional activated sludge) and U.S. (conventional activated sludge and biological trickling filter process) studies (15,19,20), but still higher than the rate in Brazilian studies, where no SaV was detected in final effluents (conventional activated sludge) (18). General detection rates in Tunisia are lower than those in South Africa (17), higher than those in Italy (16), and similar to those in Brazil (18).…”

Section: Discussioncontrasting

confidence: 72%

“…Effluents showed a detection rate of 23.9%, which is low compared to rates in Japanese (conventional activated sludge) and U.S. (conventional activated sludge and biological trickling filter process) studies (15,19,20), but still higher than the rate in Brazilian studies, where no SaV was detected in final effluents (conventional activated sludge) (18). General detection rates in Tunisia are lower than those in South Africa (17), higher than those in Italy (16), and similar to those in Brazil (18).…”

Section: Discussioncontrasting

confidence: 55%

“…The most utilized detection method for SaV is reverse transcription-quantitative PCR (RT-qPCR), which also allows the quantification of positive samples. However, information about SaV in the environment, such as the density in wastewater and removal efficiency in wastewater treatment processes, is scarce, and studies are limited to countries such as Japan, Italy, South Africa, Brazil, or the United States (15)(16)(17)(18)(19). The aim of the present study was to extend the limited knowledge about the prevalence of SaV in Tunisia, using for this purpose wastewater samples.…”

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confidence: 99%

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Sapovirus in Wastewater Treatment Plants in Tunisia: Prevalence, Removal, and Genetic Characterization

Varela

Ouardani

Kato

et al. 2018

Appl Environ Microbiol

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(SaV), from the family, is a genus of enteric viruses that cause acute gastroenteritis. SaV is shed at high concentrations with feces into wastewater, which is usually discharged into aquatic environments or reused for irrigation without efficient treatments. This study analyzed the incidence of human SaV in four wastewater treatment plants from Tunisia during a period of 13 months (December 2009 to December 2010). Detection and quantification were carried out using reverse transcription-quantitative PCR (RT-qPCR) methods, obtaining a prevalence of 39.9% (87/218). Sixty-one positive samples were detected in untreated water and 26 positive samples in processed water. The Dekhila plant presented the highest contamination levels, with a 63.0% prevalence. A dominance of genotype I.2 was observed on 15 of the 24 positive samples that were genetically characterized. By a Bayesian estimation algorithm, the SaV density in wastewater was estimated using left-censored data sets. The mean value of log SaV concentration in untreated wastewater ranged between 2.7 and 4.5 logs. A virus removal efficiency of 0.2 log was calculated for the Dekhila plant as the log ratio posterior distributions between untreated and treated wastewater. Multiple quantitative values obtained in this study must be available in quantitative microbial risk assessment in Tunisia as parameter values reflecting local conditions. Human sapovirus (SaV) is becoming more prevalent worldwide and organisms in this genus are recognized as emerging pathogens associated with human gastroenteritis. The present study describes novel findings on the prevalence, seasonality, and genotype distribution of SaV in Tunisia and Northern Africa. In addition, a statistical approximation using Bayesian estimation of the posterior predictive distribution ("left-censored" data) was employed to solve methodological problems related with the limit of quantification of the quantitative PCR (qPCR). This approach would be helpful for the future development of quantitative microbial risk assessment procedures for wastewater.

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Section: Discussioncontrasting

confidence: 72%

Section: Discussioncontrasting

confidence: 55%

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confidence: 99%

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Sapovirus in Wastewater Treatment Plants in Tunisia: Prevalence, Removal, and Genetic Characterization

Varela

Ouardani

Kato

et al. 2018

Appl Environ Microbiol

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“…Thereafter, data on the occurrence of these viruses in aquatic environments have been accumulated rapidly within the past decade owing to development and/or improvement of PCR methods for the detection of these viruses in environmental samples (Kitajima and Gerba 2015;Oka et al 2015). Recent environmental studies carried out in multiple geographical regions have collectively demonstrated a high prevalence of both sapoviruses and AiV-1 worldwide (Kitajima et al 2010a(Kitajima et al , 2011aSano et al 2011;Fioretti et al 2016;Varela et al 2016;Haramoto and Kitajima 2017), indicating that these viruses might be more prevalent in the environment than previously appreciated.…”

Section: Introductionmentioning

confidence: 99%

Temporal variations in genotype distribution of human sapoviruses and Aichi virus 1 in wastewater in Southern Arizona, United States

et al. 2018

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“…In addition, the GV.2 genotype was found to be circulating during an outbreak of food-borne gastroenteritis in Japan 37 . In Brazil, there were reports of a GV.1 genotype in a fecal sample collected during a surveillance study in Rio de Janeiro during 2012-2014 38 .…”

Section: Discussionmentioning

confidence: 99%

Detection and genotyping of human adenovirus and sapovirus in children with acute gastroenteritis in Belém, Pará, between 1990 and 1992: first detection of GI.7 and GV.2 sapoviruses in Brazil

Costa

Siqueira

Portal

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Introduction: Acute gastroenteritis (AGE) is one of the most common causes of morbidity and mortality, especially among children from developing countries. Human adenovirus (HAdV) and sapovirus (SaV) are among the agents that cause AGE. The present study aimed to detect and genotype HAdV and SaV in 172 fecal samples from children with AGE, collected during a surveillance study carried out in a low-income community in Belém, Pará, between 1990 and 1992. Methods: HAdV was detected by nested PCR, using primers Hex1deg/Hex2deg and NeHex3deg/NeHex4deg. SaV was assayed by reverse transcription PCR (RT-PCR), nested PCR, and quantitative PCR. The nucleotide sequence was determined by direct cycle sequencing. Results: Overall, 43% (74/172) of samples were positive for HAdV, of which 70.3% (52/74) were sequenced and classified as belonging to five different species, mostly A and F. For SaV, positivity was 5.2% (9/172) and genotypes GI.1, GI.7, GII.1, and GV.2 were detected. Conclusions: The present results reinforce the need for further studies to obtain epidemiological data about the circulation of these viruses in Brazil, especially in the Amazon Region, where data from the early 1990's are scarce. Furthermore, the study describes for the first time the detection of SaV genotypes GI.7 and GV.2 in Brazil, showing that these types circulated in the region more than 25 years ago.

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Occurrence of human sapoviruses in wastewater and stool samples in Rio De Janeiro, Brazil

Abstract: SaV diagnosis should be considered in hospitalized children with AGE and the higher positive rate detection in environmental samples suggests that SaV infection could be underestimated or associated with asymptomatic cases.

Cited by 30 publications

References 42 publications

Sapovirus in Wastewater Treatment Plants in Tunisia: Prevalence, Removal, and Genetic Characterization

Sapovirus in Wastewater Treatment Plants in Tunisia: Prevalence, Removal, and Genetic Characterization

Temporal variations in genotype distribution of human sapoviruses and Aichi virus 1 in wastewater in Southern Arizona, United States

Detection and genotyping of human adenovirus and sapovirus in children with acute gastroenteritis in Belém, Pará, between 1990 and 1992: first detection of GI.7 and GV.2 sapoviruses in Brazil

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