Occurrence of ‘Candidatus Phytoplasma aurantifolia’ (16SrII group) in Bolivia

Abstract

During a survey for phytoplasma at the PROINPA Foundation, Cochabamba, Bolivia in June 2008, plants of podocarpus (Podocarpus macrophyllus) showed symptoms of shortened internodes, leaf size reduction and proliferation compared to healthy ones, while those of rose (Rosa sp.) exhibited little leaf and yellowing. Both podocarpus and rose are widely distributed ornamental plants in the urban area of Cochabamba, and symptoms were observed in at least 20% of plants of each species surveyed at PROINPA. In addition, symptoms of leaf deformation, crinkling, and curling were displayed by wild plants of tomatillo (Physalis ixocarpa) growing nearby. Three plants with symptoms and one symptomless plant respectively of each species were collected. A nested PCR with universal 16S rRNA gene primers R16mF2 ⁄ R1 and fU5 ⁄ rU3 was carried out from total DNAs extracted from collected plant material. All plants with symptoms yielded PCR amplicons (~880 bp), whilst the symptomless plants did not. PCR products were purified (Wizard, Promega)

Pest categorisation of the non‐EU phytoplasmas of Cydonia Mill., Fragaria L., Malus Mill., Prunus L., Pyrus L., Ribes L., Rubus L. and Vitis L.

“…; Gypsophila paniculata(Gera et al, 2007); Helianthus spp (Mulpuri and Muddanuru, 2016)(Arocha et al, 2009a); Lactuca sativa(Cai et al, 2016); Manihot esculenta(Arocha et al, 2009a); Medicago sativa (Al-Subhi et al, 2018); Nicotiana tabacum(Schneider et al, 1999); Passiflora edulis,(Arocha et al, 2009a); Pelargonium capitatum(Lee et al, 2010); Phaseolus vulgaris(Arocha et al, 2009b); Phoenix dactilifera(Omar et al, 2018b); Rosa spp (Arocha et al, 2010)(Omar et al, 2018a); Spinacia olearia(Al- Subhi et al, 2018);…”