OCCURRENCE OF VIBRIO SPP. IN LOBSTER AND CRAB FROM THE PERSIAN GULF

Abstract

A total of 132 samples, including 100 lobsters and the 32 crab samples, were studied. A biochemical protocol was applied for the identification of the Vibrio isolates. Polymerase chain reaction (PCR) was used to confirm the isolated strains and to detect the tdh and trh genes of Vibrio parahaemolyticus. Vibrio spp. were detected in 25% of the samples analyzed in this study. Among the Vibrio isolates, Vibrio vulnificus was the species most frequently detected (13.6%), followed by Vibrio alginolyticus (9.09%), Vibrio mimicus (4.5%), Vibrio parahaemolyticus and Vibrio harveyi (3.03%). Vibrio cholerae was not found in the samples. PCR showed that none of the V. parahaemolyticus strains isolated contained the gene for thermostable direct hemolysin (tdh) and TDH‐related hemolysin (trh). The isolates demonstrated strong resistance to commonly used antibiotics. The results indicate that lobster and crab from the Persian Gulf regularly contain pathogens relevant to public health. PRACTICAL APPLICATIONS Lobster and crab are important fishery products of Persian Gulf coastal provinces of Iran. A large portion of the products were exported to other countries, especially European Union countries. Vibrio spp. such as Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio cholera are the major seafood‐borne gastroenteritis‐causing bacteria and are frequently associated with the consumption of improperly cooked seafood. The epidemiology of vibriosis in Iran is essentially unknown, and, to the authors' knowledge, the prevalence rate of Vibrio spp. in lobster and crab in Iran has never been reported. This study suggests that lobster and crab from the Persian Gulf can represent health risks for human.

Prevalence of Vibrio parahaemolyticus , and Vibrio vulnificus in blue crabs (Callinectes sapidus ), seawater and sediments of the Maryland Coastal Bays

“…Raissy et al . () did not detect the presence of V. parahaemolyticus trh + in lobsters or crab. Only 10% of crab, 20% of haemolymph, 11% of sediment and 2% of water samples were positive for both tdh + and trh + V. parahaemolyticus .…”

“…Our results are consistent with the results of previous studies which reported low incidence of tdh + and trh + V. parhaemolyticus in crabs, sediment and seawater (Duan and Su ; Raissy et al . ).…”

“…Hypoxia is a common condition in the blue crab environment and further research in this area is warranted. Raissy et al (2012) studied the occurrence of Vibrio spp. in crab and lobsters from the Persian Gulf.…”

Evaluation of marine macro alga, Ulva fasciata against bio-luminescent causing Vibrio harveyi during Penaeus monodon larviculture

“…Among the infectious diseases the luminescent disease causing Vibrio harveyi is one of the most important bacterial pathogen, capable of causing higher mortality among the marine invertebrates (Vezzulli et al, 2010). In the last two decades, mass mortalities (80-100%) among Penaeid shrimps resulting from V. harveyi infections were frequently reported in hatcheries (Raissy et al, 2011) and grow-out ponds (Zhou et al, 2012). V. harveyi has been established as well-known bacterium to produce extra cellular products indicating its virulence factors such as luminescence, proteases, phospholipases, lipases, siderophores, chitinases and hemolysins (Soto-Rodriguez et al, 2012).…”

Bactericidal Efficacies of an Aquatic Disinfectant Tablet Composed to Calcium Hypochlorite Against Vibrio anguillarum and Streptococcus iniae

“…The genus Vibrio includes gram-negative, facultative anaerobic, non-spore-forming bacilli that are oxidase positive and halophilic 1) . Several species of Vibrio can cause foodborne infection, usually associated with eating undercooked seafood 2) .…”