Occurrence of IgE in foals: Evidence for transfer of maternal IgE by the colostrum and late onset of endogenous IgE production in the horse

Wagner, Bettina; Flaminio, Julia B.F.; Hillegas, Julie; Leibold, W.; Erb, Hollis N.; Antczak, Douglas F.

doi:10.1016/j.vetimm.2005.10.007

Cited by 28 publications

(17 citation statements)

References 43 publications

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“…Similarly, enriched expression of IGF2BP3 and NR3C2 during early life has not been investigated. The expression profiles of IGHG7 and IGHE are consistent with previous studies that detect low mRNA and protein amounts at birth and document robust production over the following months [43,66,67]. Expression of granzyme B has been positively correlated with age in human infant lung samples, generally in agreement with the findings described above [68].…”

Section: Resultssupporting

confidence: 90%

“…The reported IgE + cells detected in the first days after birth are the result of maternal IgE proteins bound to the foal’s peripheral blood leukocytes, and endogenous IgE proteins are not produced until 9 months of age [66]. The transcriptome dataset in this study showed low but detectable IGHE transcripts on day 1, followed by a 100-fold increase (p<0.0001) in mRNA expression in day 42 foal samples, and another 100-fold increase (p<0.0001) in mRNA expression in adult samples, indicating that IGHE transcription occurs at low levels early in life.…”

Section: Resultsmentioning

confidence: 99%

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Transcriptome analysis of immune genes in peripheral blood mononuclear cells of young foals and adult horses

et al. 2018

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During the neonatal period, the ability to generate immune effector and memory responses to vaccines or pathogens is often questioned. This study was undertaken to obtain a global view of the natural differences in the expression of immune genes early in life. Our hypothesis was that transcriptome analyses of peripheral blood mononuclear cells (PBMCs) of foals (on day 1 and day 42 after birth) and adult horses would show differential gene expression profiles that characterize natural immune processes. Gene ontology enrichment analysis provided assessment of biological processes affected by age, and a list of 897 genes with ≥2 fold higher (p<0.01) expression in day 42 when compared to day 1 foal samples. Up-regulated genes included B cell and T cell receptor diversity genes; DNA replication enzymes; natural killer cell receptors; granzyme B and perforin; complement receptors; immunomodulatory receptors; cell adhesion molecules; and cytokines/chemokines and their receptors. The list of 1,383 genes that had higher (p<0.01) expression on day 1 when compared to day 42 foal samples was populated by genes with roles in innate immunity such as antimicrobial proteins; pathogen recognition receptors; cytokines/chemokines and their receptors; cell adhesion molecules; co-stimulatory molecules; and T cell receptor delta chain. Within the 742 genes with increased expression between day 42 foal and adult samples, B cell immunity was the main biological process (p = 2.4E-04). Novel data on markedly low (p<0.0001) TLR3 gene expression, and high (p≤0.01) expression of IL27, IL13RA1, IREM-1, SIRL-1, and SIRPα on day 1 compared to day 42 foal samples point out potential mechanisms of increased susceptibility to pathogens in early life. The results portray a progression from innate immune gene expression predominance early in life to adaptive immune gene expression increasing with age with a putative overlay of immune suppressing genes in the neonatal phase. These results provide insight to the unique attributes of the equine neonatal and young immune system, and offer many avenues of future investigation.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Transcriptome analysis of immune genes in peripheral blood mononuclear cells of young foals and adult horses

et al. 2018

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“…Neonatal foal basophils become equipped with Fc-receptor bound IgE within the first few hours after birth. These IgE antibodies originate from the dam, are transferred to the neonate in the colostrum, and can be found on basophils in the neonatal circulation for the first 2–3 months of life [18]. Crosslinking of receptor-bound IgE leads to profound IL-4 production in, and secretion from, the neonatal basophils.…”

Section: Introductionmentioning

confidence: 99%

Neonatal Immunization with a Single IL-4/Antigen Dose Induces Increased Antibody Responses after Challenge Infection with Equine Herpesvirus Type 1 (EHV-1) at Weanling Age

et al. 2017

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Neonatal foals respond poorly to conventional vaccines. These vaccines typically target T-helper (Th) cell dependent B-cell activation. However, Th2-cell immunity is impaired in foals during the first three months of life. In contrast, neonatal basophils are potent interleukin-4 (IL-4) producers. The purpose of this study was to develop a novel vaccine triggering the natural capacity of neonatal basophils to secrete IL-4 and to evaluate if vaccination resulted in B-cell activation and antibody production against EHV-1 glycoprotein C (gC). Neonatal vaccination was performed by oral biotinylated IgE (IgE-bio) treatment at birth followed by intramuscular injection of a single dose of streptavidin-conjugated gC/IL-4 fusion protein (Sav-gC/IL-4) for crosslinking of receptor-bound IgE-bio (group 1). Neonates in group 2 received the intramuscular Sav-gC/IL-4 vaccine only. Group 3 remained non-vaccinated at birth. After vaccination, gC antibody production was not detectable. The ability of the vaccine to induce protection was evaluated by an EHV-1 challenge infection after weaning at 7 months of age. Groups 1 and 2 responded to EHV-1 infection with an earlier onset and overall significantly increased anti-gC serum antibody responses compared to control group 3. In addition, group 1 weanlings had a decreased initial fever peak after infection indicating partial protection from EHV-1 infection. This suggested that the neonatal vaccination induced a memory B-cell response at birth that was recalled at weanling age after EHV-1 challenge. In conclusion, early stimulation of neonatal immunity via the innate arm of the immune system can induce partial protection and increased antibody responses against EHV-1.

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“…32 Other mare-associated factors and colostral proteins may play an important role in preventing neonatal sepsis, but their significance with respect to FPT is unclear. [35][36][37][38] A significant advantage of IR spectroscopy is that although it is possible to measure equine IgG concentrations, 24 it may be more usefully employed to capture data on additional unknown markers (i.e. new diagnostic information) or elucidate the significance of the other non-IgG markers mentioned before.…”

Section: Discussionmentioning

confidence: 99%

Feasibility of infrared spectroscopy with pattern recognition techniques to identify a subpopulation of mares at risk of producing foals diagnosed with failure of transfer of passive immunity

Riley¹,

McClure

Low-Ying

et al. 2012

Aust Veterinary J

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Occurrence of IgE in foals: Evidence for transfer of maternal IgE by the colostrum and late onset of endogenous IgE production in the horse

Cited by 28 publications

References 43 publications

Transcriptome analysis of immune genes in peripheral blood mononuclear cells of young foals and adult horses

Transcriptome analysis of immune genes in peripheral blood mononuclear cells of young foals and adult horses

Neonatal Immunization with a Single IL-4/Antigen Dose Induces Increased Antibody Responses after Challenge Infection with Equine Herpesvirus Type 1 (EHV-1) at Weanling Age

Feasibility of infrared spectroscopy with pattern recognition techniques to identify a subpopulation of mares at risk of producing foals diagnosed with failure of transfer of passive immunity

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