Occurrence of imazalil-resistant biotype of Penicillium digitatum in China and the resistant molecular mechanism

Zhu, Jinwen; Xie, Qingyun; Li, Hongye

doi:10.1631/jzus.2006.as0362

Cited by 47 publications

(38 citation statements)

References 9 publications

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“…Fruit was wounded (2-wounds per fruit) at the equatorial side with a sterile stainless steel scalpel where each wound was about 4 mm long and 2 mm deep (Zhu et al 2006). 15 µl of conidiospore suspension from each isolate were inoculated under aseptic conditions into each wound using a micropipette.…”

Section: Fruit Inoculation With Conidiospores and Plant Extractsmentioning

confidence: 99%

In Vitro and In Vivo Activity of Selected Plant Crude Extracts and Fractions Against Penicillium Italicum

Kanan¹,

Al-Najar²

2009

Journal of Plant Protection Research

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Abstract:The objective of this study is to evaluate (in vitro and in vivo) seven plant extracts and their liquid fractions against four Penicillium italicum isolates. The in vitro study revealed that a concentration 520 µg/ml of crude extract of sticky fleabane leaves or cinnamon bark generated maximum percentage inhibition of 54% and 43%, respectively, against tested fungal isolates. A concentration of 130 µg /ml from each extract (except harmal and garlic where, 390 µg/ml were required) caused complete inhibition of fungal growth of isolates Pi.1 and Pi.3 infecting orange fruit. A concentration of 130 µg/ml of nightshade fruit, fenugreek or sticky fleabane extract inhibited completely the growth of isolates Pi.3 and Pi.5 infecting lemon fruits, whereas a concentration of 390 µg/ml was required to inhibit the growth of isolate Pi.1. Methanolic fractions of cinnamon, garlic or sticky fleabane completely inhibited the growth of fungal isolates. The IC 50 values for these fractions were found to be in the range of: 11.2-24; 30.25-31.50; 25.0-36.0 µg/ml, respectively.A concentration 20 µg/ml of cinnamon hexane fraction inhibited the growth of the fungal isolates, with IC 50 values of 13, 13.75, 14 and 13 µg/ml, respectively, obtained against isolates Pi.1, Pi.3, Pi.5 and Pi.6. The nightshade hexane fraction completely inhibited the growth of isolates Pi.1 and Pi.3 with IC 50 values of 80 and 37.5 µg/ml, respectively. Cinnamon aqueous fraction completely inhibited the growth of isolates Pi.1 and Pi.5 (IC 50 were 61.25 and 58.5 µg/ml, respectively).

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Section: Fruit Inoculation With Conidiospores and Plant Extractsmentioning

confidence: 99%

In Vitro and In Vivo Activity of Selected Plant Crude Extracts and Fractions Against Penicillium Italicum

Kanan¹,

Al-Najar²

2009

Journal of Plant Protection Research

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“…Mutações nos genes β-tubulina e CYP51 têm sido descritas como responsáveis pela resistência de Penicillium spp. a tiabendazol e a imazalil, respectivamente (Schmidt et al, 2006;Zhu et al, 2006). A frequência encontrada de isolados de P. digitatum resistentes aos fungicidas, principalmente ao tiabendazol, é preocupante, pois populações resistentes poderiam proliferar facilmente e também desenvolver resistência múltipla.…”

Section: Resultados E Discussão Doenças Pós-colheitaunclassified

“…de imazalil. Estas doses de fungicidas correspondem às chamadas doses de resistência, obtidas experimentalmente, e nas quais se considera que unicamente os isolados resistentes são capazes de crescer (Brown, 1990;Holmes & Eckert, 1999;Zhu et al, 2006). O tiabendazol e o imazalil, assim como o pentabiótico veterinário, foram adicionados ao meio de cultura BDA fundente.…”

Section: Determinação Da Frequência De Isolados De Penicillium Spp Runclassified

“…et Sacc. (Brown, 1975), segundo a qual, a quantidade de esporos presentes nos packinghouses influiu significativamente nos níveis de doenças pós-colheita (Tuset, 1987;Eckert & Eaks, 1989;Zhu et al, 2006). Em levantamento da micoflora em packinghouses de citros paulistas foi constatado predomínio dos gêneros de fungos Cladosporium e Penicillium (Fischer et al, 2009a).…”

Section: Introductionunclassified

“…Estudos desenvolvidos, principalmente a partir de 1970, têm constatado que o fenômeno de resistência é uma das principais causas do fracasso de muitos tratamentos com fungicidas em pós-colheita (Houck, 1977;Boubaker et al, 2009). A grande maioria dos estudos se refere, por sua importância econômica, a P. digitatum e P. italicum Wehmer frente aos fungicidas do grupo dos benzimidazóis, ortofenilfenato de sódio e imazalil, e descrevem casos, tanto de resistência simples, como de resistência cruzada e múltipla (Bus et al, 1991;Schmidt et al, 2006;Zhu et al, 2006;Boubaker et al, 2009). Amostragens do ambiente de packinghouses de citros paulistas constataram que 39% dos isolados de P. digitatum apresentavam resistência ao fungicida tiabendazol e 1% ao imazalil (Fischer et al, 2009a).…”

Section: Introductionunclassified

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Doenças pós-colheita em laranja 'Valência' e caracterização da população fúngica em pomares orgânicos e convencionais

Fischer¹,

Zanette²,

Spósito³

et al. 2011

Trop. plant pathol.

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RESUMOA agricultura orgânica busca atender a demanda crescente por alimentos saudáveis, produzidos sem agroquímicos. Este trabalho objetivou: a) caracterizar as doenças pós-colheita em laranjas 'Valência' provenientes de cultivo orgânico e convencional; b) caracterizar a micoflora ambiental nos pomares citrícolas e c) detectar a presença de isolados de Penicillium spp. resistentes aos fungicidas tiabendazol e imazalil nos pomares. Frutos coletados nos pomares e no mercado atacadista de São Paulo (CEAGESP) foram armazenados durante 14 dias a 25ºC e 85% de UR. A incidência de doenças foi avaliada visualmente a cada dois-três dias. A micoflora ambiental foi amostrada com placas de Petri, contendo meio batata-dextrose-ágar acrescido ou não dos fungicidas. As maiores incidências de doenças foram observadas nas laranjas orgânicas em relação as do sistema convencional. A principal doença detectada foi a podridão peduncular de lasiodiplodia. A micoflora total do ambiente, assim como os principais gêneros fúngicos não diferiram significativamente entre os pomares amostrados. Cladosporium foi o gênero fúngico mais abundante nos pomares. A frequência de isolados de P. digitatum resistentes a tiabendazol foi semelhante nos pomares, com média de 47,3% dos isolados. A frequência de isolados de P. digitatum resistentes a imazalil foi baixa (2,6%), não sendo constatados isolados resistentes a tiabendazol + imazalil. Palavras-chave: Citrus sinensis, Penicillium spp., citros, podridões, resistência a fungicidas. ABSTRACT Postharvest diseases in orange fruits and characterization of the fungal population in organic and conventional farmersOrganic farming aims to meet growing demand for healthy foods, produced without the use of pesticides. The purposes of this work were a) to characterize postharvest diseases in 'Valencia' oranges grown under organic and conventional conditions; b) to characterize the environmental mycoflora in citrus orchards and c) to detect the presence of Penicillium spp. isolates resistant to fungicides thiabendazole and imazalil in the orchards. Citrus fruit collected at the orchards and at the wholesale market of São Paulo (CEAGESP) were stored during 14 days at 25ºC and 85% UR. The incidence of postharvest diseases was evaluated visually every two-three days. The environmental mycoflora was sampled according to the gravimetric method, using Petri dishes containing potato-dextrose-agar medium, supplemented or not with fungicides. The highest incidence of diseases was observed in oranges sampled in organic orchards in comparison to the conventional system. The most frequent postharvest disease was lasiodiplodia stem-end rot. The environmental mycoflora and the population of the major genera of fungi did not differ significantly among the sampled orchards. Cladosporium was the most prevailing genus in the orchards. The frequency of P. digitatum isolates resistant to thiabendazole was similar in orchards, with an average of 47.3%. The frequency of P. digitatum isolates resistant to imazalil was low (2.6%), and...

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Assessment of selection and resistance risk for demethylation inhibitor fungicides in Aspergillus fumigatus in agriculture and medicine: a critical review

Gisi

2013

Pest Management Science

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Occurrence of imazalil-resistant biotype of Penicillium digitatum in China and the resistant molecular mechanism

Cited by 47 publications

References 9 publications

In Vitro and In Vivo Activity of Selected Plant Crude Extracts and Fractions Against Penicillium Italicum

In Vitro and In Vivo Activity of Selected Plant Crude Extracts and Fractions Against Penicillium Italicum

Doenças pós-colheita em laranja 'Valência' e caracterização da população fúngica em pomares orgânicos e convencionais

Assessment of selection and resistance risk for demethylation inhibitor fungicides in Aspergillus fumigatus in agriculture and medicine: a critical review

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