Occurrence of infectious Laryngotracheitis Virus (ILTV) in 2009-2013 in the State of São Paulo - Brazil

Abstract

Infectious laryngotracheitis is a very important respiratory disease because it causes significant economic losses in the poultry industry. The target of ILTV infections is the respiratory system, and the main organ in which the virus remains latent is the trigeminal ganglia. However, the virus has demonstrated tropism for other organs as well. The present study was conducted to determine the presence of Infectious Laryngotracheitis Virus (ILTV) in the state of São Paulo. Samples submitted to LABOR-USP during the last four years (2009)(2010)(2011)(2012)(2013) analyzed by a nested/PCR technique. Out of the 682 samples from layers tested for LTIV, 12.46 % were positive, and derived from in both traditional (trachea and trigeminal ganglion) and untraditional (cecal tonsils, digestive tract and kidneys) organs utilized for ILTV diagnosis.

Attenuated infectious laryngotracheitis virus vaccines differ in their capacity to establish latency in the trigeminal ganglia of specific pathogen free chickens following eye drop inoculation

“…Nested PCR was used in the current study to detect viral DNA. This technique has been an important molecular tool for the detection of ILTV DNA in TG due to its high level of sensitivity [25,55–57]. However, detection of latency using PCR-based methods has two limitations: i) inability to differentiate between lytic and latent genomes and ii) incapability of predicting reactivation potential of the detected viruses in sites of latency.…”

Evaluación de Tres Programas de Vacunación Contra Laringotraqueitis Infecciosa Aviar Usando Dos Vacunas Vectorizadas Comerciales en Pollos de Engorde

“…La LTI ha sido reportada en muchos países, siendo considerada principalmente como endemia en regiones de crianza intensiva, en aves de múltiples edades y alojadas en galpones con alta densidad poblacional (Parra et al, 2015). En el Perú fue reportada por primera vez en agosto de 2008 (OIE, 2008).…”

<b>Isolamento em ovos embrionados e cultura celular do vírus da Laringotraqueíte Infecciosa e a padronização de um PCR em Tempo Real para a detecção do gene ICP4 deste vírus</b>

“…O vírus pode invadir o nervo trigêmeo durante a fase lítica da infecção, resultando numa infecção latente que pode permanecer durante toda a vida do animal e alguns fatores estressantes como o alojamento de outras aves e o início da postura pode causar a reativação, replicação e a eliminação do vírus (HUGHES et al, 1989;. Estudos experimentais mostraram que o vírus pode ser detectado em outros órgãos como o coração, o fígado, o baço, o pulmão, o rim, a língua, o timo, o proventrículo, o pâncreas, o duodeno, o jejuno, o íleo, o ceco, as tonsilas cecais, a bursa de Fabricius e o cérebro (OLDONI et al, 2009;WANG et al, 2013;PARRA et al, 2015a).…”