Occurrence of integrons and antimicrobial resistance genes among Salmonella enterica from Brazil

Peirano, Gisele; Agersø, Yvonne; Aarestrup, Frank Møller; Reis, Eliane Moura Falavina dos; Rodrigues, Dália dos Prazeres

doi:10.1093/jac/dkl248

Cited by 64 publications

(52 citation statements)

References 21 publications

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“…The detection rate of Int2 in Shigella in this study (100%) was higher than in other reports, including the frequencies of 86.6% in other studies in Brazil 3 and 76.9% in Japan 11 and was similar to the frequency in Korea 12 and Italy. 13 However, the frequency of Int1 in Shigella in this study (92%) was higher than that of Brazil (6.7%), 3 Korea (14.9%) 12 and Japan (3.8%). 11 Gene cassettes conferring resistance to some commonly used antibiotics in the past, such as TT and streptomycin, have been discovered and it is known that resistance genes for new antibiotics used to treat shigellosis have become part of a gene cassette.…”

contrasting

confidence: 68%

“…Concomitantly, the excretion of the pathogen in stools is shortened significantly by antibiotic therapy, reducing the spread of infection. 3 New research suggests that gene cassettes and integrons may be one mechanism involved in transmission of multidrug resistance genes in many Gram negative bacteria. 4,5 In Shigella spp., the dissemination of resistance-inducing genes is mostly facilitated by the ability of the bacteria to acquire transposons or plasmids that might contain integrons and DNA sequences that consist of two conserved segments (5¢-CS and 3¢-CS) separated by a variable region that usually comprises one or more gene cassettes.…”

mentioning

confidence: 99%

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Prevalence of integrons in Shigella sonnei from Brazil

Stehling²,

Angelini

et al. 2010

J Antibiot

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contrasting

confidence: 68%

mentioning

confidence: 99%

Prevalence of integrons in Shigella sonnei from Brazil

Stehling²,

Angelini

et al. 2010

J Antibiot

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“…Polymerase chain reaction (PCR) amplification of class 1 and 2 integrons and antimicrobial resistance genes were performed as described previously [20]. One to four colonies of the tested isolates on blood agar were suspended in 100µl of TE buffer and lysed as previously described [20].…”

Section: Molecular Mechanisms Of Resistancementioning

confidence: 99%

“…One to four colonies of the tested isolates on blood agar were suspended in 100µl of TE buffer and lysed as previously described [20]. The lysates were used as template in PCR to detect strA, strB and aadA in streptomycin resistant isolates, bla-TEM in ampicillin resistant isolates, sul2 and sul3 in sulphamethoxazole resistant isolates, tet…”

Section: Molecular Mechanisms Of Resistancementioning

confidence: 99%

“…and tet(39) in tetracycline resistant isolates, cmlA, catA1 and floR (primers for floR: Flor-1, 5'-ATGGCAGGCGATATTCATTA-3'; flor-2: 5'-AAACGGGTTGTCACGATCAT-3') in chloramphenicol resistant isolates, acc(3)-IV in gentamicin resistant isolates, qnrA, qnrB, qnrC, qnrD, qnrS, aac(6')Ib and qepA in isolates resistant to ciprofloxacin (≥ 0.125mg/L) but sensitive to nalidxic acid with MIC ≤ 32 mg/L [20,[22][23][24][25]. The variable regions of Class 1 and Class 2 integrons were amplified and representatives of the different sized amplicons sequenced to characterise their gene contents as described by Peirano et al [21].…”

Section: (A) Tet(b) Tet(c) Tet(d) Tet(e) Tet(g)mentioning

confidence: 99%

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Antibiotic resistance and resistance genes in Escherichia coli from poultry farms, southwest Nigeria

Adelowo

Fagade

Agersø

2014

J Infect Dev Ctries

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Introduction: This study investigated the mechanisms of resistance in 36 E. coli isolated from waste, litter, soil and water samples collected from poultry farms in Southwestern Nigeria. Methodology: Minimum inhibitory concentration (MIC) distributions of the isolates were determined using the methods of the Clinical and Laboratory Standard Institute and resistance genes detected by PCR. Results: A total of 30 isolates (94%) showed resistance to more than one antimicrobial. Percentage resistance was: tetracycline 81%, sulphamethoxazole 67%, streptomycin 56%, trimethoprim 47 %, ciprofloxacin 42%, ampicillin 36%, spectinomycin 28%, nalidixic acid 25%, chloramphenicol 22%, neomycin 14%, gentamicin 8%, amoxicillin-clavulanate, ceftiofur, cefotaxime, colistin, florfenicol and apramycin 0%. Resistance genes found among the isolates include bla-TEM (85%), sul2 (67%), sul3 (17%), aadA (65%), strA (70%), strB (61%), catA1 (25%), cmlA1 (13%), tetA (21%) and tetB (17%). Class 1 and 2 integrons were found in five (14%) and six (17%) isolates, respectively, while one isolate was positive for both classes of integrons. Seven out of eight isolates with resistance to ciprofloxacin and MIC ≤ 32 mg/L to nalidixic acid contained qnrS genes. Conclusions: Our findings provided additional evidence that the poultry production environment in Nigeria represents an important reservoir of antibiotic resistance genes such as qnrS that may spread from livestock production farms to human populations via manure and water.

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Occurrence, quantification, pulse types, and antimicrobial susceptibility of Salmonella sp. isolated from chicken meat in the state of Paraná, Brazil

et al. 2019

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The aim of this work was to verify the occurrence, quantification, pulse types, and antimicrobial susceptibility profiles of Salmonella sp. isolated from chicken meat produced and marketed in the state of Paraná, considered to be the state with the highest production of poultry meat in Brazil. Ninety-five of 300 (31.5%) frozen cuts of chicken were found to contain Salmonella sp., and 98 different isolates of Salmonella sp. were cultured from the positive samples. Quantification showed low Salmonella sp. loading, ranging from 0.12 to 6.4 MPN/g. The antimicrobial resistance test was performed against 16 agents from 6 different classes. All isolates were sensitive to meropenem, imipenem, chloramphenicol, and amikacin. The highest resistance rates were observed for nalidixic acid (95%), tetracycline (94%), doxycycline (94%), ampicillin (87%), amoxicillin with clavulanic acid (84%), ceftriaxone (79%), and ciprofloxacin (76%). A total of 84 (85.7%) of the isolates were identified with a multidrug resistant profile, 13 of which were found to have encoding genes extended-spectrum beta-lactamase (ESBL), especially bla CTX-M-2 e bla TEM-1. The major serovars identified were S. Typhimurium (43%) and S. Heidelberg (39%). The third most isolated serovar was S. Ndolo (6%), without previous reports of its presence in poultry meat in Brazil. Molecular characterization of S. Typhimurium and S. Heidelberg isolates by pulsed field gel electrophoresis (PFGE) showed a clonal relationship between all isolates of the same serovar (genetic similarity greater than 80%). Isolates of S. Typhimurium and S. Heidelberg with 100% similarity were found in up to five different geographic regions of the state, showing the potential for the spread of this pathogen in the Paraná poultry chain. Epidemiological surveys like this are important to understand the dynamics of dissemination and to monitor the prevalence of pathogens in the final products of poultry chains. In addition, to know the resistance profile of strains of Salmonella sp. present in food that contributes to the adoption of faster and more effective therapeutic measures, when necessary. Keywords bla CTX-M-2. bla TEM-1. PFGE. Sequencing. Salmonella Ndolo. mMPN

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Occurrence of integrons and antimicrobial resistance genes among Salmonella enterica from Brazil

Cited by 64 publications

References 21 publications

Prevalence of integrons in Shigella sonnei from Brazil

Prevalence of integrons in Shigella sonnei from Brazil

Antibiotic resistance and resistance genes in Escherichia coli from poultry farms, southwest Nigeria

Occurrence, quantification, pulse types, and antimicrobial susceptibility of Salmonella sp. isolated from chicken meat in the state of Paraná, Brazil

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