Occurrence of lectins and hydrophobicity of bacteria obtained from biofilm of hospital catheters and water pipes

Fiorina, J.C.; Weber, Mary M.; Block, Jason

doi:10.1046/j.1365-2672.2000.01143.x

Cited by 7 publications

(3 citation statements)

References 51 publications

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“…Among all the isolated strains, maximum hydrophobic interaction was shown by the strains of P. aeruginosa. Similar studies were conducted by Fiorina et al [72], who isolated bacteria from biofilms of water distribution pipes and colonised catheters from hospitalized patients. Cell surface hydrophobicity is a major parameter for controlling the adhesion of bacteria to surgical tools, medical implants, and also within the environment [73][74][75].…”

Section: Page 11 / 18supporting

confidence: 64%

Prevalence of antibiotic-resistant, toxic metal-tolerant and biofilm-forming bacteria in hospital surroundings

Nath¹,

Sinha²,

Singha³

et al. 2020

Environ Anal Health Toxicol

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Pseudomonas aeruginosa and Bacillus sp. are ubiquitous in hospital wastewater discharges. The infections caused by P. aeruginosa become complicated, as they acquire the ability to resist many classes of antibiotics. Some strains of P. aeruginosa has been reported to survive for months on dry surfaces, and it can persist and grow in contaminated antimicrobial handwash containing triclosan, making it a critical concern for hospital staff [5]. Infections with Bacillus sp. are rare, but they are able to produce spores and grow haemolytically on blood agar plates (β-hemolysis). Gram-positive Bacilli can cause many types of infections and are spread to humans in a variety of ways [6,7]. Some of the frequently encountered species include B.

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Section: Page 11 / 18supporting

confidence: 64%

Prevalence of antibiotic-resistant, toxic metal-tolerant and biofilm-forming bacteria in hospital surroundings

Nath¹,

Sinha²,

Singha³

et al. 2020

Environ Anal Health Toxicol

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“…Co‐aggregation between bacterial cells of the same or different species has been studied to some extent in oral biofilms (Kolenbrander et al ., 2002) and the disruption of co‐aggregation by specific sugars (Davey et al ., 2003) suggests that many of these interactions are mediated by lectin‐like proteins and specific sugar residues present in bacterial cell envelopes. Lectins have also been found in bacteria obtained from biofilms of hospital catheters and water pipes (Fiorina et al ., 2000). Purified LecB is also known to agglutinate E. coli O128B12 and certain marine bacteria (Sudakevitz and Gilboa‐Garber, 1982).…”

Section: Discussionmentioning

confidence: 99%

The galactophilic lectin, LecA, contributes to biofilm development in Pseudomonas aeruginosa

Diggle

Stacey

Dodd

et al. 2006

Environmental Microbiology

310

276

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LecA (PA-IL) is a cytotoxic lectin and adhesin produced by Pseudomonas aeruginosa which binds hydrophobic galactosides with high specificity and affinity. By using a lecA-egfp translation fusion and immunoblot analysis of the biofilm extracellular matrix, we show that lecA is expressed in biofilm-grown cells. In static biofilm assays on both polystyrene and stainless steel, biofilm depth and surface coverage was reduced by mutation of lecA and enhanced in the LecA-overproducing strain PAO-P47. Biofilm surface coverage by the parent strain, PAO-P47 but not the lecA mutant on steel coupons was also inhibited by growth in the presence of either isopropyl-beta-D-thiogalactoside (IPTG) or p-nitrophenyl-alpha-D-galactoside (NPG). Furthermore, mature wild-type biofilms formed in the absence of these hydrophobic galactosides could be dispersed by the addition of IPTG. In contrast, addition of p-nitrophenyl-alpha-L-fucose (NPF) which has a high affinity for the P. aeruginosa LecB (PA-IIL) lectin had no effect on biofilm formation or dispersal. Planktonic growth of P. aeruginosa PAO1 was unaffected by the presence of IPTG, NPG or NPF, nor was the strain able to utilize these sugars as carbon sources, suggesting that the observed effects on biofilm formation were due to the competitive inhibition of LecA-ligand binding. Similar results were also obtained for biofilms grown under dynamic flow conditions on steel coupons, suggesting that LecA contributes to P. aeruginosa biofilm architecture under different environmental conditions.

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“…Attachment is facilitated by the expression of cell surface polymers that alter cell surface properties (3,15). Properties that enhance attachment include increases in whole-cell hydrophobicity (14) and the abilities to coaggregate (25,36) and autoaggregate (17). Recently, it was shown, using a simple recirculating tank model (35), that freshwater biofilms formed at high shear rates contained a high proportion of bacterial species that were not detectable in the surrounding fluid phase.…”

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confidence: 99%

Shear Rate Moderates Community Diversity in Freshwater Biofilms

Rickard

McBain

Stead

et al. 2004

Appl Environ Microbiol

150

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The development of freshwater multispecies biofilms at solid-liquid interfaces occurs both in quiescent waters and under conditions of high shear rates. However, the influence of hydrodynamic shear rates on bacterial biofilm diversity is poorly understood. We hypothesized that different shear rates would significantly influence biofilm diversity and alter the relative proportions of coaggregating and autoaggregating community isolates. In order to study this hypothesis, freshwater biofilms were developed at five shear rates (<0.1 to 305 S ؊1 ) in a rotating concentric cylinder reactor fed with untreated potable water. Eubacterial diversity was assessed by denaturing gradient gel electrophoresis (DGGE) and culturing on R2A agar. Fifty morphologically distinct biofilm strains and 16 planktonic strains were isolated by culturing and identified by partial 16S rRNA gene sequencing, and their relatedness was determined by the construction of a neighbor-joining phylogenetic tree. Phylogenetic and DGGE analyses showed an inverse relationship between shear rate and bacterial diversity. An in vitro aggregation assay was used to assess the relative proportions of coaggregating and autoaggregating species from each biofilm. The highest proportion of autoaggregating bacteria was present at high shear rates ( Microorganisms colonize a wide range of environments as spatially organized, taxonomically diverse, multispecies biofilm communities (1,20,44). In potable water distribution systems, many bacterial species, including members of the Proteobacteria, Actinobacteria, low-GϩC-content gram-positive bacteria, and Cytophaga-Flavobacterium-Bacterioides group, readily adhere to surfaces to form multispecies biofilms (29,41). The abilities of these taxonomically diverse organisms to attach to surfaces and codevelop within multispecies biofilms are imperative for their survival and persistence within flowing environments (4,33,44). Few published articles have described the effect of shear on the bacterial composition and diversity of freshwater biofilms. Evidence is emerging, however, that at high fluid velocities with associated high shear rates, multispecies communities are less diverse than those developed at lower shear rates (9,23,35,42). In order to adhere to surfaces or surface-attached cells and subsequently to form biofilms, bacteria in high-velocity flowing systems must overcome shear stress at the fluid-surface interface (7, 11). Attachment is facilitated by the expression of cell surface polymers that alter cell surface properties (3, 15). Properties that enhance attachment include increases in whole-cell hydrophobicity (14) and the abilities to coaggregate (25, 36) and autoaggregate (17). Recently, it was shown, using a simple recirculating tank model (35), that freshwater biofilms formed at high shear rates contained a high proportion of bacterial species that were not detectable in the surrounding fluid phase. In addition, a larger proportion of biofilm strains than of their planktonic counterparts were able to coaggr...

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Occurrence of lectins and hydrophobicity of bacteria obtained from biofilm of hospital catheters and water pipes

Cited by 7 publications

References 51 publications

Prevalence of antibiotic-resistant, toxic metal-tolerant and biofilm-forming bacteria in hospital surroundings

Prevalence of antibiotic-resistant, toxic metal-tolerant and biofilm-forming bacteria in hospital surroundings

The galactophilic lectin, LecA, contributes to biofilm development in Pseudomonas aeruginosa

Shear Rate Moderates Community Diversity in Freshwater Biofilms

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