Occurrence of Liver Hydatid Cysts in a Donkey and Molecular Characterization of Echinococcus equinus

Kesik, Harun Kaya; Kilinc, Seyma Gunyakti; Şimşek, Sami; Gül, Abdurrahman

doi:10.1645/19-3

Cited by 14 publications

(14 citation statements)

References 17 publications

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“…Today, there are a lot of reports indicating the presence of E. equinus from different intermediate hosts worldwide (Thompson and McManus 2002;Boufana et al 2012). E. equinus have been detected from mule and donkey according to reports from Turkey (Simsek and Cevik 2014;Kesik E. equinus is usually developed into sterile small cysts in the intermediate hosts; other studies report a quite high rate of fertility and welldeveloped hydatid cysts (Ponce Gordo and Cuesta 1998;Varcasia et al 2008;Simsek and Cevik 2014).…”

Section: Discussionmentioning

confidence: 99%

Human and animal cystic echinococcosis in Konya, Turkey: molecular identification and the first report of E. equinus from human host in Turkey

et al. 2021

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Cystic echinococcosis is a neglected, zoonotic disease in Turkey. The disease is commonly seen in rural areas where the local population is in close contact with livestock and dogs. This research aimed to molecularly identify of hydatid cysts in cattle and human isolates from Konya, Turkey. Following sample collection, direct microscopy was performed. After direct examination, total DNA was extracted, and positive PCR products of cox 1 mitochondrial gene (~875 bp) were sequenced. A total of 83 hydatid cysts (cattle n = 57 and human n = 26), 82 were identified as Echinococcus granulosus sensu stricto (G1-G3 genotypes), and one human isolate was characterized as Echinococcus equinus (G4 genotype). Fertility rates of cysts belonging to cattle for liver and lung cysts were 93.3% and 80%, respectively. Out of 26 human originated isolates, 18 (69.2%) of cysts were found to be fertile. To the best of our knowledge, this is the first report of E. equinus from human host in Turkey.

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Section: Discussionmentioning

confidence: 99%

Human and animal cystic echinococcosis in Konya, Turkey: molecular identification and the first report of E. equinus from human host in Turkey

et al. 2021

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“…A recent study in Oman performed on slaughtered goats showed the predominance of infection in lungs 53.4% (Al-Kitani et al ., 2014), another study conducted in Oman showed the high prevalence in lungs 82.9% (Al-Kitani et al ., 2015). Moreover, in a recent study conducted in Turkey, 23 out of 30 hydatid cysts were found in the lungs (Kesik et al ., 2019 a ; b). The predominance of infection in lungs is that the E. granulosus s.l.…”

Section: Discussionmentioning

confidence: 99%

“…In a recent study out of 12 sheep and 10 human isolates of E. granulosus , 19 isolates have shown the presence of E. granulosus s.s. (Snábel et al ., 2009). Another study has been conducted on 120 isolates from two provinces (Elazig and Erzincan) of Turkey, 114 isolates have shown CE infection caused by E. granulosus s.s. through 12S rRNA-PCR while the other 6 samples have also shown the occurrence of E. granulosus s.s after the analysis of mt-CO1 gene (Kesik et al ., 2019 a , b ). Moreover, another study has been conducted on 58 isolates (3 sheep, 13 cattle and 42 humans) in Turkey, E. granulosus s.s. (G1 genotype) have been detected in all cattle and sheep samples however E. granulosus s.s. (G1 genotype) and E. canadensis (G7) have been characterized in human isolates (Eryildiz and Sakru, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Currently, there are three Echinococcus species which are E. granulosus s.s. , E. equinus and E. canadensis (G6/G7) present in Turkey. Studies have shown the presence of E. granulosus s.s. being the prevailing species in Turkey (Simsek et al ., 2011; Kesik et al ., 2019 a , b ). Previous records show that the CE has not been eliminated in Turkish population as 14 789 cases were reported during the period 2001–2005 (Yazar et al ., 2008) and several new CE cases were recorded (Bakal et al ., 2015; Sevinc et al ., 2016).…”

Section: Introductionmentioning

confidence: 99%

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Molecular survey on cattle and sheep hydatidosis and first detection ofEchinococcus canadensis(G6/G7) in sheep in Turkey

et al. 2020

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Larval stage of genus Echinococcus is the causing agent for the zoonotic infection which is life threatening known as Echinococcosis. The purpose of this study was the identification, molecular analysis and characterization of Echinococcus spp. in sheep and cattle. The sampling was done from slaughterhouse of Elazig, Turkey. A total of 85 isolates (sheep, n = 19 and cattle, n = 66) have been collected after slaughtering. Following the gDNA isolation and PCR products of mt-CO1 gene (446 bp) of all the samples were sequenced. Out of 85 isolates, 84 were recognized as Echinococcus granulosus sensu stricto and one sheep isolate was found as Echinococcus canadensis (G6/G7 ) which is identified for the first time in Turkey. However, single nucleotide polymorphism has been observed not only in samples of different animals but also in samples collected from the same cattle. Six liver and three lung hydatid cysts have been detected in cattle. Although no nucleotide differences have been observed in the liver samples, there was single nucleotide polymorphism (C→T) in 40th nucleotide of two lung cysts. As a result of haplotype analysis, 16 haplotypes of E. granulosus s.s. were detected in 66 cattle isolates whereas 7 haplotypes of E. granulosus s.s. were identified in 19 sheep samples.

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“…Their high host specificity probably explains why both species do not have a broad geographical distribution and occur sporadically, since access of dogs to the metacestode stages in horses and cattle is infrequent, particularly as public health measures have improved in endemic areas. However, recent ME studies have demonstrated that transmission of both species is still occurring in Turkey [ 15 ], Africa, South America, and parts of Europe [ 16 ]. Interestingly, the first ME study undertaken on echinococcosis in Bhutan demonstrated local transmission of both E. ortleppi and E. granulosus [ 17 ].…”

Section: Taxonomy and Nomenclaturementioning

confidence: 99%

The Molecular Epidemiology of Echinococcus Infections

Thompson

2020

Pathogens

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Molecular epidemiology (ME) is the application of molecular tools to determine the causation of disease. With infectious diseases, such as echinococcosis, this applies to identifying and characterising the aetiological agents and elucidating host range. Such an approach has been very successful with the causative agents of echinococcosis, species of Echinococcus, initially by providing a workable and practical taxonomy and subsequently determining transmission patterns in endemic areas. This review summarises the taxonomy and nomenclature of species of Echinococcus and provides an update on ME investigations of the ecology of Echinococcus transmission, particularly in areas where more than one species of Echinococcus is maintained in cycles of transmission that may interact.

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Occurrence of Liver Hydatid Cysts in a Donkey and Molecular Characterization of Echinococcus equinus

Cited by 14 publications

References 17 publications

Human and animal cystic echinococcosis in Konya, Turkey: molecular identification and the first report of E. equinus from human host in Turkey

Human and animal cystic echinococcosis in Konya, Turkey: molecular identification and the first report of E. equinus from human host in Turkey

Molecular survey on cattle and sheep hydatidosis and first detection ofEchinococcus canadensis(G6/G7) in sheep in Turkey

The Molecular Epidemiology of Echinococcus Infections

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