Occurrence of Multidrug-Resistant (MDR) Extended-Spectrum Beta-lactamase (ESBL)-Producing Escherichia coli in Wastewater and Natural Water Sources from the Eastern Part of Uttar Pradesh, India

Satyaprakash, Kaushik; Pesingi, Pavan Kumar; Das, Annada; Vineeth, M. R.; Malik, Satya Veer Singh; Barbuddhe, Sukhadeo B.; Rawool, Deepak Bhiwa

doi:10.1007/s11270-024-06914-y

Cited by 2 publications

(1 citation statement)

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“…The bla CTX-M , bla TEM , and bla SHV genes were more prevalent than bla OXA . These findings are consistent with a recent study that reported the recovery of ESBL-producing E. coli from natural water sources [46] . The presence of bla CTX-M genes in water is often associated with highly self-transferable plasmids, indicating the transfer of ESBL-producing E. coli from sewage to rivers [47] .…”

Section: Discussionsupporting

confidence: 93%

Phylogenetic group distribution and antibiotic resistance of Escherichia coli isolates in aquatic environments of a highly populated area

Mansour,

El-Dakdouki,

Mina

2024

AIMSMICRO

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<abstract><sec> <title>Background</title> Extended-spectrum beta-lactamase (ESBL)-producing <italic>Enterobacteriaceae</italic> including <italic>Escherichia coli</italic> (<italic>E. coli</italic>), are recognized as a global public health threat due to their multidrug-resistant (MDR) phenotypes and their rapid dissemination in aquatic environments. Nevertheless, studies investigating the prevalence and antimicrobial resistance (AMR) profile of ESBL-producing <italic>E. coli</italic> in Lebanese surface water are limited. </sec><sec> <title>Objective</title> This study aimed to assess the physicochemical properties and microbial contamination load and to determine the distribution of AMR patterns of ESBL-producing <italic>E. coli</italic> in surface water samples from different sites in the North Governorate of Lebanon. </sec><sec> <title>Methods</title> Water samples were collected from 25 major sites in North Lebanon. These samples were analyzed for the presence of total coliforms, <italic>E. coli</italic>, and fecal enterococci. Phenotypic and genetic characterizations were then performed for <italic>E. coli</italic> isolates to determine their resistance patterns and phylogenetic groups. </sec><sec> <title>Results</title> Fifty-six samples out of 100 samples were positive for ESBL-producing <italic>E. coli</italic>, mostly harboring blaCTX-M (40/56, 71%) including blaCTX-M-15 (33/40, 82%), blaTEM gene (36/56, 64%), blaSHV (20/56, 36%), and blaOXA (16/56, 29%) including blaOXA-48 gene (11/16, 69%). Most ESBL-producing <italic>E. coli</italic> isolates belonged to the extra-intestinal pathogenic phylogroup B2 (40/56, 71.4%) while 10/56 (17.9%) belonged to the commensal phylogroup A. </sec><sec> <title>Conclusion</title> Our results highlight the need to implement effective water monitoring strategies to control transmission of ESBL-producing <italic>E. coli</italic> in surface water and thus reduce the burden on human and animal health. </sec></abstract>

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Section: Discussionsupporting

confidence: 93%