Occurrence of Mycoplasma bovigenitalium and Ureaplasma diversum in dairy cattle from to Pernambuco state, Brazil

Abstract

The objective of this study was to conduct an investigation of Mycoplasma bovigenitalium and Ureaplasma diversum infections in cattle in the microregion of the Ipanema Valley, state of Pernambuco, Brazil. Vaginal swabs were collected from 355 breeding cows in reproductive age and were analyzed by multiplex PCR (mPCR) and culture. An epidemiological investigation of risk factors was performed for Mollicutes. mPCR analysis showed that, 9.29% (33/355) of the cows were positive for M. bovigenitalium and 21.69% (77/355) for U. diversum; coinfection was observed in 2.81% (10/355) of the cows. The microbiological isolation showed, 81.81% (27/33) of Mycoplasma spp. and 24.67% (19/77) of Ureaplasma spp.. The risk factors related to Mollicutes infection identified were semi-intensive breeding system (OR= 4.6), pasture rent (OR= 3.6), non-isolation of animals with reproductive disorders (OR= 3.2), and natural mounting and artificial insemination (OR= 3.5). There was a significant association between Mollicutes infection and abortions in the first gestational third (P= 0.001). This is the first record of M. bovigenitalium and U. diversum infection in cows in the semiarid region of the state of Pernambuco, Brazil. Preventive measures directed to the identified risk factors can decrease the occurrence of Mollicutes in these herds.

Evaluation of in vitro Activation of Bovine Endometrial and Vaginal Epithelial and Blood Mononuclear Cells to Produce Nitric Oxide in Response to Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum

“…Present results show a lower response of cellular culture to the stimuli of M. bovis, when compared to M. Bovigenitalium, suggesting a defi ciency of mononuclear cells to recognize M. bovis. On the other hand, Mycoplasma bovigenitalium is common in semen, prepuce, vestibule and vagina of cattle, although few animals of the same herd show clinical manifestations associated to this mycoplasma [17,18]. According to the data in Table 1, all cellular cultures exposed to Mycoplasma bovigenitalium showed results regarding NO production as an effect of the stimulation, with differences from the other agents.…”

Occurrence of Ureaplasma Diversum in the Genital Tract of Female Buffaloes (Bubalus Bubalis)

“…However, the remaining samples were not sequenced, possibly due to the low amount of DNA found naturally in the samples. In addition, contamination by other microorganisms may affect the identification, and failures may occur in the process of purification (Macêdo et al, 2018). There was no growth of characteristic colonies when samples positive on PCR were cultured in specific media.…”

“…The first report of U. diversum causing reproductive conditions in females in Brazil was described by Cardoso et al (1997) who isolated the pathogen from vaginal mucous samples of cows with vulvovaginitis. In addition, other cases of vulvovaginitis, embryo death, and abortions related to this pathogen were described in cattle herds of the Paraíba (Santos et al, 2013) and Pernambuco (Santos et al, 2015;Macêdo et al, 2018). Considering the losses caused by U. diversum and the lack of studies on the presence of this pathogen in buffalo herds, we investigated the occurrence of U. diversum in the reproductive tract of female buffaloes.…”

Do Organic, Conventional, and Intensive Approaches in Livestock Farming Have an Impact on the Circulation of Infectious Agents? A Systematic Review, Focused on Dairy Cattle

“…IDs/IAs present on both CvsO and IvsE: five studies on MAP (Sn65-Sn67 CvsO [61][62][63] ; Sn10-Sn11 IvsE 64,65 ), four studies on viral infections (Sn71-Sn72 CvsO 66,67 , and Sn14-Sn15 IvsE 64,68 ), three studies on urogenital infections (Sn69-Sn70 CvsO 29,34 , and Sn13 IvsE 69 ), three studies on Campylobacter sp., (Sn60-Sn61 CvsO 70,71 , and Sn8 IvsE 72 ), four studies on ectoparasites (Sn62-Sn64 CvsO 49,24,25 , and Sn9 IvsE 73 ), two studies on zoonotic dermatophyte (Sn12 CvsO 74 , and Sn68 IvsE 74 ).…”