Occurrence of Mycoplasma synoviae on commercial poultry farms of Pernambuco, Brazil

Abstract

Pesq. Vet. Bras. 34(10):953-956, outubro 2014 953 RESUMO.-[Ocorrência de Mycoplasma synoviae em granjas comerciais da avicultura de Pernambuco.] O estado de Pernambuco é o maior produtor de ovos da região Norte e Nordeste e ocupa a segunda posição na produção de frangos de corte. Os micoplasmas são importantes patógenos aviários que causam doenças respiratórias e sinovite que resultam em grandes perdas econômicas.Objetivou-se pesquisar a ocorrência de Mycoplasma gallisepticum (MG) e Mycoplasma synoviae (MS) em frangos de corte e poedeiras comerciais no Estado de Pernambuco, Brasil. Foram colhidos fragmentos de traquéia de 55 frangos de corte sadios, 35 com sinais respiratórios e de 30 poedeiras comerciais também com sinais respiratórios, provenientes de 24 granjas, cada amostra foi composta por um "pool" de cinco aves. Para detecção de Mycoplasma gallisepticum (MG) e Mycoplasma synoviae (MS) foram utilizados o exame bacteriológico, PCR e Nested-PCR. Todas as amostras apresentaram resultados negativos no exame bacteriológico. Na PCR, sete amostras foram positivas para MS e uma para MG em amostras de aves com sinais respiratórios, sendo a amostra positiva para MG confirmada como cepa vacinal MG-F. A ocorrência de MS em aves com sinais clínicos respiratórios pode indicar ausência de barreiras sanitárias adequadas em granjas de frangos de corte e de poedeira comercial, favorecendo a sua propagação. The state of Pernambuco is the largest producer of eggs in the North and Northeast of Brazil and second one in the broiler production. Mycoplasmas are important avian pathogens, which cause respiratory and joint diseases that result in large economic losses. The aim of the present study was to investigate the occurrence of Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) in broilers and commercial laying hens in the state of Pernambuco, Brazil. Tracheal fragments were analyzed from 55 healthy broilers, 35 broilers with respiratory signs and 30 commercial laying hens with respiratory signs, from 24 commercial poultry farms, each sample was composed of a pool of five birds. The bacteriological exam, PCR and nested PCR were used for the detection of Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS). All samples were negative in bacteriological isolation. In the PCR analyses, seven samples from birds with respiratory signs were positive for MS and one was positive for MG, the latter of which was confirmed as the MG-F vaccine strain. The occurrence of MS in chickens with respiratory signs may indicate inadequate sanitary management on poultry farms, favoring the propagation of mycoplasmosis. Occurrence of

Incidence of Mycoplasma gallisepticum and Mycoplasma synoviae in broiler flocks at the Federal District of Brazil and its surrounding areas

“…The results obtained for the detection of MG are also similar to those of a study conducted at Pernambuco, in which 11 broilers and laying hens showed positive PCR and nested PCR for the presence of MG in seven samples (33.33%) (Barros et al, 2014); however, some flocks had clinical respiratory signs, unlike the flocks in the present study as evident in the ante-mortem inspection. In the state of Goiás, Minharro, Linhares, Andrade, Rocha and Santana (2001) also detected MG in 32.25% and MS in 25.80% swab samples from air sac injuries using PCR in broilers at slaughterhouses located at the municipalities of Itaberaí and Pires do Rio.…”

Characterization and Differentiation of Chicken Mycoplasma Isolates Using 16S-23S Intergenic Spacer Region Sequencing

“…Due to regular vaccination of the flocks, wild type MG prevalence is relatively low, not found using Positions where nucleotide differences exist in one sequence are denoted with the ambiguity setter code, according to the IUB. PCR by Buim et al (2009), Mettifogo et al (2013) and Barros et al (2014), 5% (Teixeira et al, 2015) and isolated from 2,67% of the flocks by Santos (2015). On the other hand, since flocks are not vaccinated for MS in Brazil, prevalence is estimated to be very high, with 79,31% (Buim et al, 2009), 68% (Mettifogo et al, 2013), 83,33% (Barros et al, 2014), 45% (Teixeira et al, 2015) and 93,34% (Santos, 2015).…”

“…PCR by Buim et al (2009), Mettifogo et al (2013) and Barros et al (2014), 5% (Teixeira et al, 2015) and isolated from 2,67% of the flocks by Santos (2015). On the other hand, since flocks are not vaccinated for MS in Brazil, prevalence is estimated to be very high, with 79,31% (Buim et al, 2009), 68% (Mettifogo et al, 2013), 83,33% (Barros et al, 2014), 45% (Teixeira et al, 2015) and 93,34% (Santos, 2015). However, isolation of pathogenic mycoplasmas may be difficulted by rapid growth of commensal strains, such as M. gallinarum and M. gallinaceum (Kleven, 2008).…”