Occurrence of penta-amines, hexa-amines and &lt;i&gt;N&lt;/i&gt;-methylated polyamines in unicellular eukaryotic organisms belonging to the phyla &lt;i&gt;Heterokontophyta&lt;/i&gt; and &lt;i&gt;Labyrinthulomycota&lt;/i&gt; of the subdomain &lt;i&gt;Stramenopiles&lt;/i&gt;

Hamana, Koei; Furuchi, Takemitsu; Nakamura, Tsuzuri; Hayashi, Hidenori; Niitsu, Masaru

doi:10.2323/jgam.2016.05.001

Cited by 3 publications

(1 citation statement)

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“…Although norspermidine is a relatively rare polyamine, it has been found in a number of aquatic plants and lower eukaryotes. In aquatic plants, norspermidine is synthesized by polyamine oxidases as a byproduct of thermospermine metabolism at very low levels [ 13 , 14 , 16 , 40 ]. Therefore, it is unlikely that aquatic plants could produce enough norspermidine to support an association with V .…”

Section: Discussionmentioning

confidence: 99%

Relative contributions of norspermidine synthesis and signaling pathways to the regulation of Vibrio cholerae biofilm formation

et al. 2017

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The polyamine norspermidine is one of the major polyamines synthesized by Vibrionales and has also been found in various aquatic organisms. Norspermidine is among the environmental signals that positively regulate Vibrio cholerae biofilm formation. The NspS/MbaA signaling complex detects extracellular norspermidine and mediates the response to this polyamine. Norspermidine binding to the NspS periplasmic binding protein is thought to inhibit the phosphodiesterase activity of MbaA, increasing levels of the biofilm-promoting second messenger cyclic diguanylate monophosphate, thus enhancing biofilm formation. V. cholerae can also synthesize norspermidine using the enzyme NspC as well as import it from the environment. Deletion of the nspC gene was shown to reduce accumulation of bacteria in biofilms, leading to the conclusion that intracellular norspermidine is also a positive regulator of biofilm formation. Because V. cholerae uses norspermidine to synthesize the siderophore vibriobactin it is possible that intracellular norspermidine is required to obtain sufficient amounts of iron, which is also necessary for robust biofilm formation. The objective of this study was to assess the relative contributions of intracellular and extracellular norspermidine to the regulation of biofilm formation in V. cholerae. We show the biofilm defect of norspermidine synthesis mutants does not result from an inability to produce vibriobactin as vibriobactin synthesis mutants do not have diminished biofilm forming abilities. Furthermore, our work shows that extracellular, but not intracellular norspermidine, is mainly responsible for promoting biofilm formation. We establish that the NspS/MbaA signaling complex is the dominant mediator of biofilm formation in response to extracellular norspermidine, rather than norspermidine synthesized by NspC or imported into the cell.

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