Occurrence of Poly-β-Hydroxybutyric Acid in Aerobic Gram-Negative Bacteria

SUMRlARYThe ultrastructure of Lupinus luteus L. and Ornithopus sativus Brot. root nodules derived from infection by Rhizobium lupini strain ~2 5 was examined. In both cases the associations produced completely effective nodules but their gross morphology was markedly different. Bacteria within the 0. sativus nodules were enclosed in groups by a membranous envelope, and the bacteria had electron translucent regions. Bacteria within the L. luteus nodules were enclosed singly and did not exhibit similar electron translucent regions. Starch granules were observed within infected cells of L. luteus but not within uninfected cells; the reverse was observed in 0. sativus cells.

Section: Introductionmentioning

confidence: 95%

Host Influence on the Ultrastructure of Root Nodules of Lupinus luteus and Ornithopus Sativus

Kidby

Goodchild

1966

“…Bokman, Levine & Lusby (1957) found that it metabolizes glucose, probably through the pathway of Entner & Doudoroff (1952); this is the characteristic mode of glucose dissimilation by several species of aerobic pseudomonads. Finally, Levine & Wolochow (1960) showed that this organism accumulates poly-P-hydroxybutyrate as a cellular reserve material, a trait that is also not uncommon in non-fluorescent species among the aerobic pseudomonads (Forsyth, Hayward & Roberts, 1958).…”

Section: Introductionmentioning

confidence: 99%

A Comparative Study of Pseudomonas pseudomallei and Bacillus mallei

Redfearn

Palleroni

Stanier

1966

108

SUMMARYA comparative study of many strains of Pseudom,onas pseudomallei and Bacillus mallei has shown that these two species are very similar with respect to their nutritional and biochemical properties, thus confirming earlier claims of a relationship between them, based on such criteria as pathological and serological properties. P. pseudomallei is in all respects a typical and nutritionally highly versatile member of the genus Pseudomonas. In view of this fact we propose that B. mallei should also be placed in the genus Pseudomonas, even though it is a permanently non-motile bacterium. The ecology and possible evolutionary relationships between the two species are discussed in the light of the present findings.

“…Burdon (1946) noted that Rhizobium possessed more sudanophilic granules than most Gramnegative genera. Others (Forsyth, Hayward & Roberts, 1958; Smithies, Gibbons & Bayley, 1955) have reported rhizobium as one of many organisms that contain a polymer of p-hydroxybutyric acid. This polymer was first described in Bacillus megaterium by Lemoigne (1925), and was found by Kbpits & Peaud-Lenoel (1952) to consist of P-hydroxybutyric acid molecules linked through the carboxyl and hydroxyl groups into chains of various lengths.…”

Section: Introductionmentioning

confidence: 99%

Some Features of the Fine Structure and Chemical Composition of Rhizobium trifolii

Vincent¹,

Humphrey

North

1962

SUMMARYThe conspicuous, large, high refractive index, sudanophilic granules of Rhixobium trifolii appeared to be aggregations of polymeric p-hydroxybutyric acid, probably closely associated with the cytoplasm. They became more conspicuous as the organism aged, provided that carbohydrate was in excess. A well-grown culture contained 40-50 yo polymer, based on cell dry weight. Relatively large cytoplasmic granules (50-80 mp) were a feature of this organism whether in fixed and sectioned cells or in material shadowed after mechanical disintegration.Classical ' double ' ( ? lipoprotein) membranes were demonstrated both for the cell wall and the cytoplasmic membrane. Carefully fixed and embedded material often showed an accumulation of material between the two double membranes, especially a t one or both ends, without any evidence of gross damage in the sectioned organisms.