Occurrence of potentially pathogenic vibrios in the marine environment of the Straits of Messina (Italy)

Gugliandolo, Concetta; Carbone, M.; Fera, M. T.; Irrera, G.P.; Maugeri, Teresa L.

doi:10.1016/j.marpolbul.2005.02.045

Cited by 13 publications

(7 citation statements)

References 24 publications

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“…It is known that among vibrios, these three species can adapt themselves to adverse conditions e.g., organic matter limited environments by means of survival strategies such as adhering to different substrata [28,35] hence survival of these pathogenic vibrios in wastewater treatment plants is possible. The most prevalent species detected was V. fluvialis followed by V. vulnificus and V. parahaemolyticus .…”

Section: Resultsmentioning

confidence: 99%

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Occurrence of Vibrio Pathotypes in the Final Effluents of Five Wastewater Treatment Plants in Amathole and Chris Hani District Municipalities in South Africa

Nongogo

Okoh

2014

IJERPH

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We assessed the occurrence of Vibrio pathogens in the final effluents of five wastewater treatment plants (WWTPs) located in Amathole and Chris Hani District Municipalities in South Africa over a 12 months period between September 2012 and August 2013 using standard membrane filtration technique followed by cultivation on thiosulphate citrate-bile salts-sucrose (TCBS) agar. The identities of the presumptive Vibrio isolates were confirmed using polymerase chain reaction (PCR) including delineation into V. parahaemolyticus, V. vulnificus and V. fluvialis pathotypes. The counts of Vibrio spp. varied with months in all the study sites and ranged in the order of 101 and 104 CFU/100mL. Vibrio distribution also showed seasonality with high counts being obtained in autumn and spring (p < 0.05). Prevalence of Vibrio spp. among the five WWTPs also differed significantly (p < 0.05). Of the 300 isolates that were confirmed as belonging to the Vibrio genus, 29% (86) were V. fluvialis, 28% (84) were V. vulnificus and 12% (35) were V. parahaemolyticus. The isolation of Vibrio pathogens from the final effluent suggests that this pathogen is in circulation in some pockets of the population and that the WWTPs under study do not efficiently remove bacterial pathogens from the wastewater and consequently are threats to public health.

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Section: Resultsmentioning

confidence: 99%

“…Previous reports have articulated the isolation of Vibrio species from different niches and geographical regions. In marine environments of Italy Gugliandolo et al [35] found V. vulnificus as a dominant species, while Sousa et al [36] isolated V. parahaemolyticus and V. cholera in oysters collected in Brazil. Also Tuning et al .…”

Section: Resultsmentioning

confidence: 99%

Occurrence of Vibrio Pathotypes in the Final Effluents of Five Wastewater Treatment Plants in Amathole and Chris Hani District Municipalities in South Africa

Nongogo

Okoh

2014

IJERPH

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“…2006). Vibrio vulnificus was isolated in June from mussels, and the 16S rRNA gene of V. vulnificus was detected only in shellfish collected in June and September, confirming that the presence of this pathogen depends on the water’s temperature and tend to be more abundant when water temperature exceeds 17°C (Gugliandolo et al. 2005, 2008; Maugeri et al.…”

Section: Discussionmentioning

confidence: 89%

“…Vibrio vulnificus is a halophilic, pathogenic bacterium generally present in low numbers in the Mediterranean Sea and in Straits of Messina, and its isolation by culture method is limited to the warmest periods (Gugliandolo et al. 2005; Maugeri et al.…”

Section: Discussionmentioning

confidence: 99%

Conventional and molecular methods to detect bacterial pathogens in mussels

Gugliandolo

Lentini

Spanò

et al. 2010

Letters in Applied Microbiology

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Aim: To detect Aeromonas spp., Salmonella spp., Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus in mussels and water samples from a farming area, conventional and molecular methods were applied to enrichment cultures. Methods and Results: The aerolysin gene (aero) of Aeromonas spp., the invasion plasmid antigen B (ipaB) gene of Salmonella spp., the enterotoxin secretion protein (epsM) gene of V. cholerae, the species‐specific region of 16S rRNA gene of V. vulnificus, the 16S–23S rDNA (IGS) gene of V. parahaemolyticus and the pR72H fragment of V. parahaemolyticus were amplified by multiplex polymerase chain reaction (PCR) assays on DNA extracted from enrichment cultures. The haemolysin gene (tdh) of pathogenic V. parahaemolyticus was also amplified. Conventional culture method allowed the isolation of V. parahaemolyticus and V. vulnificus from water and mussels. The genes aero, epsM and 16S rRNA of V. vulnificus were occasionally detected in the enrichment cultures. In mussels, the ipaB and IGS genes were detected from June to September and from April to November, respectively. All genes, except aero, were amplified from mussels collected in September, when pathogenic V. parahaemolyticus (tdh+) strains were also isolated. Conclusions: Multiplex‐PCR assays were more sensitive and faster than conventional procedures. Significance and Impact of the Study: The results emphasize the need of an accurate and rapid detection of bacterial pathogens in mussels to protect human health.

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“…Vibrios tolerate a wide range of salinity (Farmer et al, 2005), with high levels detected at moderate salinities (5± 25 ppt) and an inverse correlation between salinity and abundance at elevated (>25 ppt) salinities (Martinez-Urtaza et al, 2008;Huq et al, 2005;Castaneda et al, 2005;Parvathi et al, 2004;Randa et al, 2004;Louis et al, 2003;DePaola et al, 2000;Motes et al, 1998;Chowdhury et al, 1992;O'Neill et al 1992;Miller et al, 1982;Singelton et al 1982;Kaper et al, 1979). Other factors such as dissolved organic carbon (DOC), suspended solids and plankton and copepods may also play a role (Gugliandolo et al, 2005;Jones and Summer Brason, 1998;Watkins and Cabelli, 1985). These correlations suggest direct effects of salt and other environmental factors on Vibrio growth and or survival in estuarine ecosystems.…”

Section: Pathogenic Vibrio Speciesmentioning

confidence: 99%

Microbial contamination and shellfish safety

Jones

2009

Shellfish Safety and Quality

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Occurrence of potentially pathogenic vibrios in the marine environment of the Straits of Messina (Italy)

Cited by 13 publications

References 24 publications

Occurrence of Vibrio Pathotypes in the Final Effluents of Five Wastewater Treatment Plants in Amathole and Chris Hani District Municipalities in South Africa

Occurrence of Vibrio Pathotypes in the Final Effluents of Five Wastewater Treatment Plants in Amathole and Chris Hani District Municipalities in South Africa

Conventional and molecular methods to detect bacterial pathogens in mussels

Microbial contamination and shellfish safety

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