Occurrence of putative endornaviruses in non-cultivated plant species in South Louisiana

Herschlag, Rachel; Escalante, Cesar; Souto, Eliezer Rodrigues de; Khankhum, Surasak; Okada, Ryo; Valverde, R. A.

doi:10.1007/s00705-019-04270-5

Cited by 8 publications

(3 citation statements)

References 30 publications

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“…Although endornaviruses are not associated with visible pathogenic effects, their presence correlates with, e.g., faster seed germination, longer bean pods, higher seed weight, etc. (Fukuhara, 2019; Herschlag et al, 2019). Hence, it is very likely that endornaviruses have been positively selected for during breeding for economically important traits, but they rely on certain host factors to maintain their persistence and prevent viral disease.…”

Section: Discussionmentioning

confidence: 99%

Virus Latency and the Impact on Plants

et al. 2019

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Plant viruses are thought to be essentially harmful to the lives of their cultivated crop hosts. In most cases studied, the interaction between viruses and cultivated crop plants negatively affects host morphology and physiology, thereby resulting in disease. Native wild/non-cultivated plants are often latently infected with viruses without any clear symptoms. Although seemingly non-harmful, these viruses pose a threat to cultivated crops because they can be transmitted by vectors and cause disease. Reports are accumulating on infections with latent plant viruses that do not cause disease but rather seem to be beneficial to the lives of wild host plants. In a few cases, viral latency involves the integration of full-length genome copies into the host genome that, in response to environmental stress or during certain developmental stages of host plants, can become activated to generate and replicate episomal copies, a transition from latency to reactivation and causation of disease development. The interaction between viruses and host plants may also lead to the integration of partial-length segments of viral DNA genomes or copy DNA of viral RNA genome sequences into the host genome. Transcripts derived from such integrated viral elements (EVEs) may be beneficial to host plants, for example, by conferring levels of virus resistance and/or causing persistence/latency of viral infections. Studies on viral latency in wild host plants might help us to understand and elucidate the underlying mechanisms of latency and provide insights into the raison d’être for viruses in the lives of plants.

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Section: Discussionmentioning

confidence: 99%

Virus Latency and the Impact on Plants

et al. 2019

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“…Some of these crops are rice, barley, pepper, and common bean (Wakarchuk & Hamilton 1985, Zabalgogeazcoa & Gildow 1992, Fukuhara et al 1993, Okada et al 2011. In a recent study from the United States (US), endornaviruses were detected in non-cultivated plant species (Herschlag et al 2019). In addition to the known host range of endornaviruses, gherkin was found to be a new host for CmEV as the result of this study.…”

Section: Resultsmentioning

confidence: 99%

FIRST REPORT OF Cucumis melo endornavirus WITH A NEW HOST, THE GHERKIN (Cucumis anguria Linn.), IN TURKEY

Karanfil

Korkmaz

2020

Trakya University Journal of Natural Sciences

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More than 50 viruses causing infection in members of the family Cucurbitaceae have been identified in the world so far. Because of the development of virus detection methods, new viruses are added to the known infectious cases list every day. One of the viruses recently identified is the Cucumis melo endornavirus (CmEV) which has been reported from different countries all over the world. However, no study for determination of CmEV has been done so far in Turkey. For the purpose of this study, 59 cucurbit plants showing virus and virus-like symptoms were collected from Manisa and İzmir provinces in Turkey. The samples were tested, for presence of CmEV, by reverse-transcriptase polymerase chain reaction and CmEV infections were detected in 47 samples, of which 44 were melon (Cucumis melo Linn.), and the remaining were gherkins (C. anguria Linn.). Among the infected samples, five samples (three were melon, and two were gherkin) were chosen for sequence analysis. After evaluating the sequence analysis results, it was shown that the Turkish isolates presented 93%-99% and 93%-98% identities at the nucleotide level and 94%-99% and 91%-98% identities at the amino acid level among each other and worldwide isolates, respectively. To the best of our knowledge, this is the first report of CmEV in gherkin as a new host both in Turkey and worldwide.Özet: Dünyada gerçekleştirilen çalışmalar sonucunda kabakgil familyasındaki bitkileri enfekte eden 50'den fazla virüs varlığı tanımlanmıştır. Virüs tespit metotlarındaki gelişmelere bağlı olarak bu sayıya her geçen gün yenileri eklenmektedir. Son yıllarda tanılanan bu virüs hastalıklarından bir tanesi de Cucumis melo endornavirus (CmEV)'dır. CmEV'nin enfeksiyonu dünyanın farklı ülkelerinden bildirilmiştir. Ancak, ülkemizde şimdiye kadar CmEV varlığının belirlenmesine yönelik bir çalışma gerçekleştirilmemiştir. Bu amaçla, Manisa ve İzmir illerinden virüs ve virüsbenzeri belirti gösteren 59 kabakgil bitkisinden örnekler alınmıştır. Toplanan örnekler ters transkriptaz-polimeraz zincir reaksiyonu ile test edilmiştir. Gerçekleştirilen bu testler sonucunda 47 örnekte CmEV enfeksiyonu tespit edilmiştir. Enfekteli 47 örneğin 44 tanesi kavun (Cucumis melo Linn.), kalan 3 örnek ise acurdur (C. anguria Linn.). Enfekteli örnekler arasından, beş tanesi (üç tanesi kavun, iki tanesi acur) sekans analizleri için seçilmiştir. Gerçekleştirilen sekans analizleri sonucunda CmEV izolatları kendi aralarında nükleotit düzeyinde %93-99, amino asit düzeyinde ise %94-99 benzerlikler gösterdiği belirlenmiştir. Dünya izolatları ile yapılan benzerlik analizleri sonucunda ise nükleotit düzeyinde %93-98, amino asit düzeyinde ise %91-98 benzerlikler tespit edilmiştir. Gerçekleştirilen bu çalışma ile ülkemizde ilk kez CmEV enfeksiyonu kavun ve dünya için yeni bir konukçu kaydı olan acur bitkisinde tespit edilmiştir.

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“…In plants, endornaviruses do not cause apparent effects on the phenotype and are transmitted only vertically [2,23]. Endornaviruses have been reported in many cultivated and wild plant species [2,3,5,23].…”

Section: Introductionmentioning

confidence: 99%

Complete Nucleotide Sequence of an Endornavirus Isolated from Common Buckwheat (Fagopyrum Esculentum)

Okada¹,

Moriyama

Ogawara³

et al. 2021

Preprint

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A double-stranded RNA (dsRNA) of approximately 16 kbp was isolated from symptomless common buckwheat (Fagopyrum esculentum) plants. The size of the dsRNA suggested that it was the replicative form of an endornavirus. The dsRNA was sequenced, and it consisted of 15,677 nt containing a single open reading frame that potentially encoded a polyprotein of 5,190 aa. The polyprotein contained conserved domains for a viral methyltransferase, viral RNA helicase 1, MSCRAMM family adhesion SdrC, UDP-glycosyltransferase, and viral RNA dependent RNA polymerase 2. A site-specific nick in the plus strand was detected near the 5' end of the dsRNA. BLASTP analysis showed that the polyprotein shared the highest identity with the polyprotein of winged bean endornavirus 1. Results of phylogenetic analysis supported placing the novel virus from common buckwheat, which was provisionally named Fagopyrum esculentum endornavirus 1, in the genus Alphaendornavirus of the family Endornaviridae.

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Occurrence of putative endornaviruses in non-cultivated plant species in South Louisiana

Cited by 8 publications

References 30 publications

Virus Latency and the Impact on Plants

Virus Latency and the Impact on Plants

FIRST REPORT OF Cucumis melo endornavirus WITH A NEW HOST, THE GHERKIN (Cucumis anguria Linn.), IN TURKEY

Complete Nucleotide Sequence of an Endornavirus Isolated from Common Buckwheat (Fagopyrum Esculentum)

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