In the contemporary era, with rapid industrial growth and urbanisation, analysing the impact of human activities on soil enzyme activity becomes crucial. The purpose of this study is to assess the influence of anthropogenic pollution on enzyme activity in soil. Research on enzyme activity levels in the soil in the city of KaraBalta, Chuy Region, Kyrgyz Republic, was conducted using biochemical analyses and specific enzymatic tests. The results revealed significant diversity in catalase activity in different soil samples. Some samples exhibited high activity, while others showed low activity. These differences may be associated with oxidative stress and the ability of soil microorganisms to decompose hydrogen peroxide. Urease analysis indicated the highest activity in soil samples after 2 hours, particularly near the protective barrier of the tailings pond, suggesting intensive chemical reactions, especially near pollution sources. Research has also revealed the diversity of protease activity in soil ecosystems, where samples with high activity may more effectively break down proteins compared to samples with low activity. To achieve ecological stability of soil resources, it is necessary to develop a management strategy, including monitoring and restoring priority areas considering local characteristics, supporting biodiversity, applying sustainable agricultural methods, and combating soil erosion. Important steps also include forming a community emphasising the importance of soil resources, funding research, and collaborating with local authorities, scientists, and the business community. The results of the study can be used in developing strategies to prevent the negative consequences of soil pollution, contributing to improved ecological resilience, especially for environmental protection agencies