Occurrence of recombinant genotypes of Anisakis simplex s.s. and Anisakis pegreffii (Nematoda: Anisakidae) in an area of sympatry

Abollo, Elvira; Paggi, L.; Pascual, Santiago; D’Amelio, Stefano

doi:10.1016/s1567-1348(03)00073-x

Cited by 128 publications

(137 citation statements)

References 21 publications

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“…seem to be characteristic of the Atlantic part of the Iberian Peninsula, and have been detected in numerous other fish from this region (Abollo et al 2003, Marques et al 2006, Sequeira et al 2010. The significantly lower relative abundance of this species complex in fish from the Azores, and the again significantly lower relative abundance in Madeira, make it a good indicator of P. bogaraveo provenance.…”

Section: Biogeographical Patterns Of Distribution At the Regional Levelsupporting

confidence: 92%

Parasites as biological tags for stock identification of blackspot seabream, <i>Pagellus bogaraveo</i>, in Portuguese northeast Atlantic waters

Hermida¹,

Cruz²,

Saraiva³

2013

Sci. Mar.

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SUMMARY: In this study the following parasites were selected as biological tags for the identification of blackspot seabream (Pagellus bogaraveo) stocks in the northeast Atlantic: Diphterostomum vividum (Digenea: Zoogonidae), Anisakis simplex s.l., A. physeteris, Anisakis sp. PB-2010 (Nematoda: Anisakidae), and Bolbosoma sp. (Acanthocephala: Polymorphidae). These parasite species point to the existence of three blackspot seabream stocks in the northeast Atlantic: one in the Azores region (ICES Area X), one in continental Portuguese shelf/slope waters (ICES Area IXa), and one in the waters around Madeira (sub-area 1.2 of FAO 34, central-eastern Atlantic).

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Section: Biogeographical Patterns Of Distribution At the Regional Levelsupporting

confidence: 92%

Parasites as biological tags for stock identification of blackspot seabream, <i>Pagellus bogaraveo</i>, in Portuguese northeast Atlantic waters

Hermida¹,

Cruz²,

Saraiva³

2013

Sci. Mar.

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“…The heterogeneity was observed at those sites diagnostic for each species. The heterogeneity was only observed in areas where sympatric coexistence occurred (Abollo et al 2003, Farjallah et al 2007). ITS sequences from anisakids isolated from Baltic Sea herring had a T at position 173, whereas other A. simplex s.s. possessed a C. These herring anisakids could represent the second 'variant' in a hybrid situation; however, they did not display the corresponding polymorphic nucleotides at the other positions observed in anisakids from RVS salmon.…”

Section: Asp Ay821749 Tc--cgta Ta-cagttt--gtgtgatc-tatgt--caa Cgcaamentioning

confidence: 81%

“…Internal transcribed spacer ribosomal DNA (ITSrDNA) was amplified as described (Zhu et al 1998), and the resultant PCR products were purified by MinElute Gel Purification (Qiagen) and sequenced on a CEQ 8800 using the GenomeLab DTCS Quick Start kit (Beckman Coulter). Restriction fragment length polymorphism (RFLP) analysis was performed on purified ITS PCR products using HinfI and HhaI (D'Amelio et al 2000, Abollo et al 2003, Pontes et al 2005, Umehara et al 2007). PCR products were cloned using the TOPO TA cloning vector (Invitrogen).…”

Section: Methodsmentioning

confidence: 98%

Red vent syndrome in wild Atlantic salmon Salmo salar in Scotland is associated with Anisakis simplex sensu stricto (Nematoda: Anisakidae)

Noguera

Collins²,

Bruno³

et al. 2009

Dis. Aquat. Org.

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Simultaneous reports were received between June and July 2007 of wild Atlantic salmon Salmo salar with red, swollen, bloody vents returning to geographically diverse rivers in Scotland. By the end of September the condition, colloquially known as 'red vent syndrome' (RVS), was reported from > 50 rivers across Scotland. Fish were generally in good overall condition but the vent area showed mild to severe lesions. External characteristics of the syndrome included a swollen, raised, haemorrhagic vent and surrounding tissues, with erosion of the skin, scale loss and moderate to severe bleeding in more advanced cases. Predominantly, the fish affected were 1-sea-winter grilse; however, RVS was also recorded in 2-sea-winter salmon and sea trout S. trutta. High numbers of the nematode Anisakis Type I larvae were found infesting the discrete region of the vent, a localisation that is reported as novel for the parasite. The hypothesis that this is a different species than that commonly found in the body cavity and viscera was investigated through molecular studies. These studies failed to show evidence that the parasites infesting the vent were different from those in the body cavity, i.e. all were identified as A. simplex sensu stricto. No other disease agent was found associated with the lesions or was isolated systemically, and no mortality or prevention of spawning was recorded during the 2007 season. Possible causes, including warming environments in the North Atlantic, are hypothesised as playing a role in the development of RVS in Atlantic salmon.

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“…Using a molecular polyphasic approach such as PCR-RFLP, sequencing of nuclear and mitochondrial markers and phylogenetic analysis, we were able to specifically identify A. pegreffii in M. merluccius and L. piscatorius from Adriatic Sea waters. The unequivocal identification of A. pegreffii by using the entire ITS was possible for the specific restriction pattern obtained with the enzyme Hinf I and the diagnostic nucleotides C and C at alignment positions 475 and 491 peculiar for A. pegreffii (Abollo et al, 2003;Abe et al, 2005;Ceballos-Mendiola et al, 2010). These findings clearly demonstrated that the heterozygote pattern observed with Hinf I enzyme is due to an incomplete digestion of the amplified DNA.…”

Section: Discussionmentioning

confidence: 99%

“…consists of three sibling species, namely A. simplex sensu stricto (s.s.), A. pegreffii and A. simplex C differing in their genetic structure and in ecological traits, such as geographic distribution and host preference (Mattiucci et al, 1997). Anisakis simplex s.s. is mainly distributed in the northern Atlantic and Pacific Oceans, A. simplex C in the Pacific coasts and in the Southern hemisphere and A. pegreffii in the Mediterranean Sea and in the Southern hemisphere (Abollo et al, 2003). The precise identification of Anisakis larvae up to species level is very difficult because no obvious morphological differences have been reported especially in differentiating A. simplex s.s. from A. pegreffii.…”

Section: Introductionmentioning

confidence: 99%

A polyphasic approach with molecular phylogeny for the characterization of Anisakis pegreffii (Anisakidae: Nematoda) in fishes from Adriatic Sea

Luca¹,

Vovlas

Troccoli³

et al. 2013

Helminthologia

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SummaryIn this study we investigated the morphometric and molecular characterization of a liver encapsulated third-stage larval population of Anisakis spp. infecting Merluccius merluccius and Lophius piscatorius caught in the Adriatic Sea waters (southern Italy). A polyphasic approach based on PCR-RFLP profiles of the ITS region, mitochondrial COI (cytochrome c oxidase subunit 1), sequencing and molecular phylogeny of ITS and mitochondrial COI was used to identify Anisakis larvae collected from fish samples. PCR-RFLP analysis showed three banding pattern corresponding to the peculiar pattern of A. pegreffii. Sequence data from ribosomal ITS and mitochondrial COI were analysed by Neighbour Joining, Minimum Evolution and Maximum Parsimony methods to evaluate the phylogenetic relationships among A. simplex sensu lato. The phylogenetic trees obtained for both ITS and COI revealed the existence of three distinct clades for A. simplex sensu sricto, A. simplex C and A. pegreffii and the sequences obtained in this study clearly clustered together with A. pegreffii sequences present in the database. Histopathological observations of anisakid nematode specimens detected on the liver surface of M. merluccius are illustrated. Encapsulated specimens of the L3 stage of the nematode were similar in size and morphometry to those found into the peritoneal cavity. Anisakis larvae encapsulated on the liver surface within dense and pearl coloured envelops caused host hepatic tissue necrosis, large cavities and oedematous liver spots to the host.

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Occurrence of recombinant genotypes of Anisakis simplex s.s. and Anisakis pegreffii (Nematoda: Anisakidae) in an area of sympatry

Cited by 128 publications

References 21 publications

Parasites as biological tags for stock identification of blackspot seabream, <i>Pagellus bogaraveo</i>, in Portuguese northeast Atlantic waters

Parasites as biological tags for stock identification of blackspot seabream, <i>Pagellus bogaraveo</i>, in Portuguese northeast Atlantic waters

Red vent syndrome in wild Atlantic salmon Salmo salar in Scotland is associated with Anisakis simplex sensu stricto (Nematoda: Anisakidae)

A polyphasic approach with molecular phylogeny for the characterization of Anisakis pegreffii (Anisakidae: Nematoda) in fishes from Adriatic Sea

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