Occurrence of Siderophores in Enterococci

Lisiecki, Paweł; Wysocki, Piotr J.; Mikucki, J

doi:10.1016/s0934-8840(00)80006-7

Cited by 13 publications

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References 32 publications

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“…Until now, enterococci have been regarded as not having the ability to produce siderophores. However, it has been stated that the bacteria produce iron chelators belonging to the hydroxamate class of siderophores (Lisiecki et al, 1999). We demonstrated that production of the hydroxamate siderophores in the growth medium by both of the tested strains was induced by iron deficiency at concentration of 3.5 × 10 -7 M. Even lower free iron concentration of the human body (10 -18 M) may induce the production of hydroxamate siderophores, which may bind the iron of TR and LF and transport it into the cells.…”

Section: Discussionmentioning

confidence: 99%

“…Bacteriological media and growth conditions. Strains were grown in complex liquid medium (Lisiecki et al, 1999). Iron deficient-medium was obtained using polyaminocarboxyl resin Chelex 100 (200-400 mesh, BioRad, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Total siderophore activity in the culture supernatants was measured using chrome azurol S (CAS) (Schwyn and Neilands, 1987). Hydroxamate siderophores were assayed using a chemical-specific assay (Lisiecki et al, 1999). The results were expressed as micrograms of desferrioxamine mesylate (Sigma, USA) per millilitre of culture supernatant.…”

Section: Methodsmentioning

confidence: 99%

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Transferrin and Lactoferrin – Human Iron Sources for Enterococci

Lisiecki

2017

Polish Journal of Microbiology

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To overcome limitations in iron acquisition, enterococci have evolved a number of mechanisms to scavenge iron from the host iron-binding proteins - transferrin (TR) and lactoferrin (LF). The aim of this study was to demonstrate the mechanisms by which enterococci utilize human TR and LF bound iron. The study included two strains of Enterococcus faecalis grown in iron-deficient and iron-excess media respectively. The binding activity of both proteins was monitored using proteins labelled with 125I. The uptake of iron by enterococci was determined using 59Fe labelled proteins. Reduction of iron bound to TR and LF was assayed with ferrozine. The proteolytic cleavage of TR and LF was visualized by SDS-polyacrylamide gel electrophoresis. The siderophore activity was measured with chrome azurol S. The study revealed that enterococci use several ways to acquire iron from TR and LF, such as iron chelating siderophores, iron reduction - facilitated iron release, protein degradation - promoted iron release, and receptor mediated capture of the iron-host protein complexes. The broad spectrum of iron acquisition mechanisms used by enterococci may play a significant role in the colonization of the human body and the resulting pathogenicity.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Transferrin and Lactoferrin – Human Iron Sources for Enterococci

Lisiecki

2017

Polish Journal of Microbiology

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“…were upregulated in excess iron (Lopez et al, 2012) (Lisiecki, 2014;Lisiecki and Mikucki, 2006;Lisiecki et al, 2000;Maskell, 1980). Interestingly, E. faecalis possesses the arginine deaminase pathway (arcABCRD) which encodes for ornithine transcarboxylase, carbamate kinase, putative Crp/Fnrtype regulator, and a ornithine-arginine antiporter, which can synthesize ornithine (Barcelona-Andres et al, 2002), a cofactor required for hydroxamate-type siderophores.…”

Section: Role Of Iron In Enterococci Physiologymentioning

confidence: 99%

The role of iron in Enterococcus faecalis biofilm formation

Lam¹

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“…Manganese is also important for the oxidative stress response in E. faecalis (Colomer-Winter et al 2017, Kehres andMaguire 2003). Though studies have detected compounds with broad chelation capacity in E. faecalis, they have not been characterized in detail Mikucki 2004, Lisiecki andMikucki 2006). Moreover, iron acquisition systems for siderophore transport or heme iron transport has not been functionally characterized in E. faecalis (Miethke andMarahiel 2007, Neilands 1995).…”

Section: Introductionmentioning

confidence: 99%

Inside-out : pathogenesis of enterococcal-associated wound infections

Tay¹

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LIST OF TABLES iii LIST OF FIGURES iv LIST OF ABBREVIATIONS vii PUBLICATIONS x PRESENTATIONS xi CHAPTER 1: BACKGROUND AND SIGNIFICANCE CHAPTER 2: ENTEROCOCCAL METABOLITE CUES FACILITATE INTERSPECIES NICHE MODULATION AND POLYMICROBIAL INFECTION 2.1 Introduction and motivation 2.2 Results 2.2.1 Enhanced E. coli growth by E. faecalis is dependent on enterobactin siderophore production 2.2.2 E. coli ferrous uptake systems are not important for E. faecalis-driven E. coli growth promotion 2.2.3 Co-culture of E. faecalis and E. coli results in enhanced biofilm/biomass under iron deplete conditions 2.2.4 E. faecalis Transposon library screen and validation 2.2.5 E. faecalis Transposon library single species secondary validation 2.2.6 L-ornithine, but not branched-chain amino acids, augments E. coli biofilm formation under iron limitation 2.3 Discussions 2.4 Materials and methods 2.5 Contributions 2.6 References for Chapter 2

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Occurrence of Siderophores in Enterococci

Cited by 13 publications

References 32 publications

Transferrin and Lactoferrin – Human Iron Sources for Enterococci

Transferrin and Lactoferrin – Human Iron Sources for Enterococci

The role of iron in Enterococcus faecalis biofilm formation

Inside-out : pathogenesis of enterococcal-associated wound infections

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