Occurrence of the adenylate cyclase “G Protein” in membranes of Dictyosteliumdiscoideum

Leichtling, Ben H.; Coffman, D.S.; Yaeger, Eric S.; Rickenberg, H.V.; al-Jumaliy, W.; Haley, Boyd E.

doi:10.1016/s0006-291x(81)80137-4

Cited by 43 publications

(19 citation statements)

References 24 publications

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“…Previously, in D. discoideum a membrane-bound protein was described showing properties, which are characteristic for vertebrate G-proteins [20]. The data presented here, show that a G-protein may be functionally linked to the 'non-specific' folate receptors.…”

supporting

confidence: 60%

Guanine nucleotides modulate the ligand binding properties of cell surface folate receptors in Dictyostelium discoideum

Wit

Bulgakov

1985

FEBS Letters

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Dictyostelium discoideutn cells show 2 distinct classes of cell surface binding sites for folates. One type is non-specific, i.e., binds folic acid (FA), 2-deaminofolic acid (DAFA), and methotrexate (MTX) with similar affinity (K,, N 140 nM). Scatchard analysis of this non-specific binding type suggests either heterogeneity or negative cooperativity. Isolated D. discoideum membranes show similar binding characteristics. Guanine nucleotides changed the binding levels of [3H]MTX. In the presence of 0.1 mM GTP, the number of binding sites remains unchanged, while the affinity decreases. GDP and guanylyl imidodiphosphate (GPPNP) are required at about 20-fold higher concentration than GTP, which elicits a half-maximal effect at 15 FM. Other guanine and adenine nucleotides are ineffective up to 1 mM. These results suggests that the nonspecific cell surface receptor for folic acid interacts with a guanine nucleotide regulatory (G-) protein. Folate receptor Guanine nucleotide

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supporting

confidence: 60%

Guanine nucleotides modulate the ligand binding properties of cell surface folate receptors in Dictyostelium discoideum

Wit

Bulgakov

1985

FEBS Letters

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“…The hormone-stimulated GTP hydrolysis in different systems is inhibited following ADP-ribosylation by cholera and/or pertussis toxin [ 15,. The existence of a G protein in D. discoideum membranes has been suggested previously by Leichtling et al [22], who showed that a 42-kDa protein binds GTP and can be ADP-ribosylated by the cholera toxin.…”

Section: Introductionmentioning

confidence: 82%

Agonist‐stimulated high‐affinity GTPase in Dictyostelium membranes

Snaar-Jagalska¹,

Jakobs

Haastert³

1988

FEBS Letters

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“…There, transition appears to be due to the coupling of the occupied high-affinity receptor with a GTP-binding protein, resulting in the activation of adenylate cyclase [12]. Recently, evidence has been presented for a functional G-protein in D. discoideum [13][14][15]. Guanine nucleotides promote the transition of H-to L-sites; they also alter the affinity and number of S-sites.…”

Section: Introductionmentioning

confidence: 99%

cAMP activates adenylate and guanylate cyclase of Dictyostelium discoideum cells by binding to different classes of cell-surface receptors. A study with extracellular Ca2+

Haastert

1985

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

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Citation for published version (APA): Haastert, P. J. M. V. (1985). cAMP activates adenylate and guanylate cyclase of Dictyostelium discoideum cells by binding to different classes of cell-surface receptors. A study with extracellular Ca2+. Biochimica et Biophysica Acta %28BBA%29 -Molecular Cell Research, 846(3). DOI: 10.1016/0167-4889(85)90002-3 Copyright Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum. (ii) The K d of the S-sites was reduced from 16 nM to 5 nM (iii) The conversion of H-sites to L-sites was inhibited up to 80%. The kinetics of the cAMP-induced cAMP accumulation was not strongly altered by Ca 2+, but the amount of cAMP produced was inhibited up to 80%. The kinetics of the cAMP-induced cGMP accumulation was strongly altered; maximal levels were obtained sooner, and the K a was reduced from 15 to 3.5 nM cAMP. Ca 2+, Mg 2+ and Mn 2+ increased the number of binding sites, all with EC~ = 0.5 mM. The S-sites and the cGMP response were modified by equal Ca 2+ concentrations and by higher concentrations of Mg 2+ and Mn 2 + (ECs0 are respectively 0.4 mM, 2.5 mM and about 25 mM). The conversion of H-to L-sites and the cAMP response were specifically inhibited by Ca 2+ with EC~ = 20 /~M. It is concluded that cAMP activates guanylate cyclase through the S-sites; adenylate cyclase is activated by the H + L-sites, in which the appearance of the L-sites during the binding reaction represents the coupling of occupied surface cAMP receptors to adenylate cyclase.

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Occurrence of the adenylate cyclase “G Protein” in membranes of Dictyosteliumdiscoideum

Cited by 43 publications

References 24 publications

Guanine nucleotides modulate the ligand binding properties of cell surface folate receptors in Dictyostelium discoideum

Guanine nucleotides modulate the ligand binding properties of cell surface folate receptors in Dictyostelium discoideum

Agonist‐stimulated high‐affinity GTPase in Dictyostelium membranes

cAMP activates adenylate and guanylate cyclase of Dictyostelium discoideum cells by binding to different classes of cell-surface receptors. A study with extracellular Ca2+

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