Occurrence of the tobacco cyst nematode subspecies Globodera tabacum subsp. virginiae in France

Bardou‐Valette, Sylvie; Grenier, Éric; Montarry, Josselin

doi:10.1007/s10658-015-0741-5

Cited by 3 publications

(1 citation statement)

References 15 publications

(22 reference statements)

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“…1). These results are consistent with those of previous studies (Alenda et al, 2013;Bardou-Valette et al, 2016). The restriction patterns of Gt-j were identical to those of Gtt.…”

Section: Resultssupporting

confidence: 93%

Subspecies identification of the Japanese population of Globodera tabacum

Sakata

Sakai

Kushida

2021

Japanese Journal of Nematology

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Globodera tabacum was detected only once in Japan, from an eggplant greenhouse in 1998. This species includes three subspecies, G. tabacum tabacum, G. tabacum virginiae, and G. tabacum solanacearum. The population detected in Japan (Gt-j), however, was yet to be identified at the subspecies level. Therefore, to identify the subspecies of Gt-j, we performed a PCR-RFLP test using CLAVATA3/ endosperm surrounding region-related (CLE) peptidecoding genes and the sequence analysis of a pectate lyase gene. Restriction fragment patterns of the Gt-j CLE peptidecoding genes Cle1 and Cle5 were identical to those of G. tabacum tabacum. In addition, the sequence comparison of Pel1 showed more than 99% similarity between Gt-j and eight isolates of G. tabacum tabacum. Based on these results, we identified Gt-j as G. tabacum tabacum. Nematol. Res. 51(2), 37-40. (2021)

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“…1). These results are consistent with those of previous studies (Alenda et al, 2013;Bardou-Valette et al, 2016). The restriction patterns of Gt-j were identical to those of Gtt.…”

Section: Resultssupporting

confidence: 93%

Subspecies identification of the Japanese population of Globodera tabacum

Sakata

Sakai

Kushida

2021

Japanese Journal of Nematology

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A review of methods for nematode identification

Seesao

Merlin

Viscogliosi

et al. 2017

Journal of Microbiological Methods

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Characterization of Putative Effectors from the Cereal Cyst Nematode Heterodera avenae

et al. 2018

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Few molecular details of effectors of Heterodera avenae parasitism are known. We performed a high-throughput sequencing analysis of the H. avenae transcriptome at five developmental stages. A total of 82,549 unigenes were ultimately obtained, and 747 transcripts showed best hits to genes putatively encoding carbohydrate-active enzymes in plant-parasitic nematodes that play an important role in the invasion process. A total of 1,480 unigenes were homologous to known phytonematode effectors, and 63 putative novel effectors were identified in the H. avenae transcriptomes. Twenty-three unigenes were analyzed by qRT-PCR and confirmed to be highly expressed during at least one developmental stage. For in situ hybridization, 17 of the 22 tested putative effectors were specifically expressed and located in the subventral gland cells, and five putative novel effectors were specifically expressed in the dorsal gland. Furthermore, 115 transcripts were found to have putative lethal RNA interference (RNAi) phenotypes. Three target genes with lethal RNAi phenotypes and two of the four tested putative effectors were associated with a decrease in the number of cysts through in vitro RNAi technology. These transcriptomic data lay a foundation for further studies of interactions of H. avenae with cereal and H. avenae parasitic control.

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Occurrence of the tobacco cyst nematode subspecies Globodera tabacum subsp. virginiae in France

Cited by 3 publications

References 15 publications

Subspecies identification of the Japanese population of <i>Globodera tabacu</i><i>m </i>

Subspecies identification of the Japanese population of <i>Globodera tabacu</i><i>m </i>

A review of methods for nematode identification

Characterization of Putative Effectors from the Cereal Cyst Nematode Heterodera avenae

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