Occurrence of tomato spotted wilt and cucumber mosaic viruses in field‐grown tomato crops and associated weeds in northeastern Spain

Laviña, A.; Aramburu, J.; Moriones, Enrique

doi:10.1111/j.1365-3059.1996.tb02893.x

Cited by 27 publications

(18 citation statements)

References 10 publications

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“…To our knowledge this is the first report of natural infection of Sambucus ebulus, Cichorium intybus, Conyza canadensis, Helliotropium europaeum, Lactuca serriola, Picris echioides and Scabiosa tennuiis by PVY (Schmelzer, 1967;De Bokx and Huttinga, 1981;Katis, 1984;Edwardson and Christie, 1997;Espino de Paz et al, 1997;Sˇkoric´et al, 2000;Fletcher, 2001;Kazinczi et al, 2002), of Fumaria officinalis by CMV Francki et al, 1979;Ford et al, 1988;Lorbeer, 1989, 1991;Crescenzi et al, 1993;Lavin´a et al, 1996;Fletcher, 2001;Rodriguez-Alvarado et al, 2002), and of Scabiosa tenuiis and Urtica dioica by AMV (Jaspars and Bos, 1980;Fletcher, 2001;Kazinczi et al, 2002). Some others such as Cirsium arvense, Cyperus rotundus, Mentha pulegium, Xanthium spinosum and X. strumarium for PVY, Amaranthus blitoides, Chondrila juncea, Malva neglecta and Mentha pulegium for CMV, as well as Cyperus rotundus and Picris echioides for AMV, were found infected for the first time by ELISA, but the virus detected was not readily recoverable in bioassays.…”

Section: Discussionmentioning

confidence: 88%

A survey of tobacco viruses in tobacco crops and native flora in Greece

Chatzivassiliou¹,

Efthimiou²,

Drossos

et al. 2004

European Journal of Plant Pathology

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The most important tobacco producing areas in Greece were surveyed for virus presence, from 1997 to 2000. Tobacco seedlings or plants showing virus-like symptoms were randomly collected from seedbeds or fields, respectively, and tested by ELISA, and/or mechanical inoculation onto indicator plants. Potato virus Y (PVY), Cucumber mosaic virus (CMV) and Tobacco mosaic virus (TMV) were detected in all sampling areas, with TMV mainly found in oriental varieties. Tomato spotted wilt virus (TSWV) consisted a serious endemic virus in Northern Greece (Thrace, Central and Eastern Macedonia), whereas Alfalfa mosaic virus (AMV) was mainly found in regions, where alfalfa was cultivated in the vicinity of tobacco crops. Eggplant mottled dwarf virus (EMDV) was detected in several areas but always in very low incidence (<0.01%). Surveys were also conducted to assess the potential reservoir hosts of PVY, CMV and AMV among weeds collected from highly infected tobacco fields from 1998 to 2000. Among 3450 samples tested for PVY, plants from 17 species in 10 families were found infected. For CMV, 2891 weed samples were tested and 19 species in 12 families were positive. Assays for AMV infection were made on 961 samples and 12 species in 9 families were identified as hosts of this virus.

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Section: Discussionmentioning

confidence: 88%

A survey of tobacco viruses in tobacco crops and native flora in Greece

Chatzivassiliou¹,

Efthimiou²,

Drossos

et al. 2004

European Journal of Plant Pathology

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“…ELISA methods have been widely used for detecting and subgrouping the CMV isolates [9,21,22]. In addition, both RT-PCR and multiplex IC-RT-PCR assays were reported for differentiation of CMV subgroups I and II [10,14].…”

Section: Discussionmentioning

confidence: 99%

Multiplex RT-PCR detection of <italic>Cucumber mosaic virus</italic> subgroups and <italic>Tobamoviruses</italic> infecting Tomato using 18S rRNA as an internal control

Chen¹,

Gu²,

Wang³

et al. 2011

ABBS

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A multiplex reverse-transcription polymerase chain reaction (RT-PCR) protocol was developed for simultaneous detection and discrimination of subgroups of Cucumber mosaic virus (CMV), including its satellite RNA, Tomato mosaic virus (ToMV) and Tobacco mosaic virus (TMV), using 18S rRNA as an internal control. Species-and subgroups-specific primers designed to differentiate CMV subgroups I and II, ToMV and TMV, were assessed using the cDNA clones of viral genomes, CMV satellite RNA and 18S rRNA gene from tomato (Solanum lycopersicum L.) or tobacco (Nicotiana tobacum). Using total RNA extracted from artificial mixture of tomato leaf tissues infected by each virus, the reaction components and cycling parameters were optimized and a multiplex RT-PCR procedure was established. Six fragments of 704, 593, 512, 421, 385, 255 bp, specific to CMV subgroup II, CMV subgroup I, ToMV, TMV, satellite RNA and 18S rRNA, respectively, were simultaneously amplified. The sensitivity of the multiplex RT-PCR method for detecting CMV was 100 times higher than that of double-antibody sandwich-enzymelinked immunosorbent assay (DAS-ELISA). This method was successfully used for field detection. Among 141 samples collected from East China through tomato growth seasons, 106 single infections with one of the above isolates were detected and 13 mixed infections were found. The results showed the potential use of this method for investigating the epidemiology of viral diseases infecting tomato.

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“…In Spain, TSWV is a limiting factor in the production of economically important crops such as tomato, pepper and lettuce in many growing areas, where WFT is the main virus vector (Lacasa, 1990;Arnó et al, 1995;Laviña et al, 1996). Effective control measures to reduce the losses caused by TSWV are not available.…”

Section: Introductionmentioning

confidence: 99%

Comparative host reactions and Frankliniella occidentalis transmission of different isolates of tomato spotted wilt tospovirus from Spain

Roca¹,

Aramburu²,

Moriones³

1997

Plant Pathology

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The abilities of different isolates of tomato spotted wilt tospovirus (TSWV) collected from northeastern and eastern Spain to infect 10 host species, and to be acquired and transmitted by the western flower thrips (WFT), Frankliniella occidentalis were compared. Two isolates of TSWV from a single source plant could be separated according to the different type of local lesions they induced in Nicotiana glutinosa. Host ranges of the studied TSWV isolates were very similar, but differences were found in the symptoms induced and in their capacity to infect specific hosts systemically. Lycopersicon esculentum and Datura stramonium were evaluated for their potential as virus acquisition host species. The proportion of transmitter adult thrips obtained from WFT larvae fed on L. esculentum was greater than from D. stramonium. No differences were detected between TSWV isolates in their ability to be acquired and transmitted by WFT. No evidence was obtained of alterations in TSWV particles which could affect WFT transmissibility due to the repeated mechanical transfers used to clone the isolates. Our findings do not support the existence of pathological effects of TSWV on WFT.

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Occurrence of tomato spotted wilt and cucumber mosaic viruses in field‐grown tomato crops and associated weeds in northeastern Spain

Cited by 27 publications

References 10 publications

A survey of tobacco viruses in tobacco crops and native flora in Greece

A survey of tobacco viruses in tobacco crops and native flora in Greece

Multiplex RT-PCR detection of <italic>Cucumber mosaic virus</italic> subgroups and <italic>Tobamoviruses</italic> infecting Tomato using 18S rRNA as an internal control

Comparative host reactions and Frankliniella occidentalis transmission of different isolates of tomato spotted wilt tospovirus from Spain

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Occurrence of tomato spotted wilt and cucumber mosaic viruses in field‐grown tomato crops and associated weeds in northeastern Spain

Cited by 27 publications

References 10 publications

A survey of tobacco viruses in tobacco crops and native flora in Greece

A survey of tobacco viruses in tobacco crops and native flora in Greece

Multiplex RT-PCR detection of &lt;italic&gt;Cucumber mosaic virus&lt;/italic&gt; subgroups and &lt;italic&gt;Tobamoviruses&lt;/italic&gt; infecting Tomato using 18S rRNA as an internal control

Comparative host reactions and Frankliniella occidentalis transmission of different isolates of tomato spotted wilt tospovirus from Spain

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Multiplex RT-PCR detection of <italic>Cucumber mosaic virus</italic> subgroups and <italic>Tobamoviruses</italic> infecting Tomato using 18S rRNA as an internal control