Occurrence of Treponema spp. in porcine skin ulcers and gingiva

Karlsson, Frida; Svartström, Olov; Belák, Katinka; Fellström, Claes; Pringle, Märit

doi:10.1016/j.vetmic.2013.03.031

Cited by 29 publications

(33 citation statements)

References 22 publications

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“…detected by FISH was 69% in shoulder ulcers and 59% in ear necroses, compared to the original study by Karlsson et al (2013) where treponemes were detected in 52% of the shoulder ulcers and in 46% of the ear necroses by an ISR2-based PCR. It is however not possible to compare these results fully.…”

Section: Discussioncontrasting

confidence: 67%

“…vincentii-like (Brandt et al, 2011;Choi et al, 1997;Evans et al, 2009;Klitgaard et al, 2013;Nordhoff et al, 2008;Yano et al, 2010). T. phagedenis-like spirochetes were not found in porcine skin ulcers in the study by Karlsson et al (2013) and T. medium was only sparsely detected, but frequent occurrence of T. parvum was indicated Svartströ m et al, 2013). Unfortunately, due to difficulties in designing a specific probe, were not able to investigate the occurrence of T. parvum by FISH.…”

Section: Discussionmentioning

confidence: 70%

“…In the study by Karlsson et al (2013) several phylotypes of treponemes were detected in ear necrosis and shoulder ulcers in pigs by an ISR2-based PCR, followed by sequencing. To investigate the presence of other Treponema spp.…”

Section: Discussionmentioning

confidence: 99%

“…These pigs originated from seven different herds and two pigs from each herd were sampled. The herds were not related to the herds in Karlsson et al (2013). The biopsies were taken immediately after culling, but before scalding, from the skin of the shoulder region or the ventral margin of the ear.…”

Section: Herds Animals and Sampling Proceduresmentioning

confidence: 99%

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Identification of Treponema pedis as the predominant Treponema species in porcine skin ulcers by fluorescence in situ hybridization and high-throughput sequencing

Karlsson

Klitgaard

Jensen

2014

Veterinary Microbiology

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Section: Discussioncontrasting

confidence: 67%

Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Section: Herds Animals and Sampling Proceduresmentioning

confidence: 99%

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Identification of Treponema pedis as the predominant Treponema species in porcine skin ulcers by fluorescence in situ hybridization and high-throughput sequencing

Karlsson

Klitgaard

Jensen

2014

Veterinary Microbiology

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“…These treponemes are commonly isolated from DD skin lesions in beef and dairy cattle and the association has been reported worldwide (Walker and others 1995, Dawson 1998, Klitgaard and others 2008, Nordhoff and others 2008, Evans and others 2009, Yano and others 2010, Sullivan and others 2014a). These bacteria are now also viewed as the primary infections in contagious ovine digital dermatitis (lesions in sheep in the UK and Ireland (Demirkan and others 2001, Sayers and others 2009), analogous foot lesions in goats (Sullivan and others 2014a, b), severe non-treatable foot lesions in dairy cattle (Evans and others 2011), skin lesions in pigs (Svartstrom and others 2013, Karlsson and others 2013, 2014) and a newly reported ‘hoof disease’ in wild North American elk (Clegg and others 2014). DD associated treponemes have also been detected in horse canker lesions (Sykora and others 2014).…”

Section: Introductionmentioning

confidence: 99%

Bovine ischaemic teat necrosis: a further potential role for digital dermatitis treponemes

et al. 2016

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A recent outbreak of ischaemic teat necrosis (ITN) on mainland UK has resulted in large economic losses for dairy farmers. Typical cases start as an area of dry, thickened and encrusted skin on the medial aspect of the base of the teat, where the teat joins the udder, often with a fetid odour. The erosion spreads down the teat, often causing intense irritation, which in turn leads to more severely affected animals removing the entire teat. Due to the severity of ITN and the substantial economic costs to the industry, analyses were undertaken to ascertain if an infectious agent might be involved in the pathology. The study has considered a role for digital dermatitis (DD) treponemes in the aetiopathogenesis of ITN because, as well as being the prime bacteria associated with infectious lameness, they have been associated with a number of emerging skin diseases of cattle, including udder lesions. A high association between presence of DD-associated treponemes and incidence of ITN (19/22), compared with absence in the control population is reported. Furthermore, sequencing of the 16S rRNA gene of treponeme isolates supports the hypothesis that the identified treponemes are similar or identical to those isolated from classical foot DD lesions in cattle (and sheep). Further studies are required to allow effective targeted prevention measures and/or treatments to be developed.

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Molecular detection of Treponema species organisms in foremilk and udder cleft skin of dairy cows with digital dermatitis

Sobhy

Mahmmod

Refaai

et al. 2019

Trop Anim Health Prod

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Identification of reservoirs and transmission routes of digital dermatitis (DD) associated Treponema spp. considered an effective means for controlling DD infection in dairy cows. The objective of this study is to identify and characterize the potential reservoir niches for DD-associated Treponema spp. from healthy udder cleft skin and foremilk in lactating dairy cows. A large dairy farm was visited weekly from March to July 2015. Clinical investigation revealed that a total of 25 lame cows had DD lesions located at the plantar aspect of the interdigital cleft. A total of 75 samples, three per cow, were collected including deep swabs from DD lesions (n=25), nonaseptically collected foremilk samples (n=25) and skin swabs from udder cleft (n=25). Treponema spp. were identified using nested PCR assays and confirmed by DNA sequencing. Results revealed that Treponema phagedenis (T. Phagedenis)-like was the most identified species in the foremilk 40% (10/25), in comparison to DD lesions and udder cleft skin samples with 32% (8/25) and 20% (5/25), respectively. On the other hand, Treponema pedis (T. Pedis) was the most identified species in the udder cleft skin 80% (20/25), in comparison to DD lesions and foremilk samples with 68% (17/25) and 60% (15/25), respectively. None of the examined samples were identified by PCR as containing DNA from Treponema medium (T. Medium) or Treponema vincentii (T. vincentii)-like. To the best of our knowledge, this is the first report for detection of T. phagedenis-like and T. pedis from healthy skin of udder cleft and foremilk samples. Detection of DD Treponema spp. from udder cleft skin and foremilk samples indicates that these sites could be potential reservoirs for spirochetes involved in DD. Udder cleft skin and foremilk may have a role in transmission routes of DD Treponema in dairy farms.

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Occurrence of Treponema spp. in porcine skin ulcers and gingiva

Cited by 29 publications

References 22 publications

Identification of Treponema pedis as the predominant Treponema species in porcine skin ulcers by fluorescence in situ hybridization and high-throughput sequencing

Identification of Treponema pedis as the predominant Treponema species in porcine skin ulcers by fluorescence in situ hybridization and high-throughput sequencing

Bovine ischaemic teat necrosis: a further potential role for digital dermatitis treponemes

Molecular detection of Treponema species organisms in foremilk and udder cleft skin of dairy cows with digital dermatitis

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