Occurrence of two different species of mitoviruses in the European race of Gremmeniella abietina var. abietina, both hosted by the genetically unique Spanish population

Botella, Leticia; Tuomivirta, Tero; Vervuurt, Stephane; Diez, Julio J.; Hantula, Jarkko

doi:10.1016/j.funbio.2012.05.004

Cited by 23 publications

(36 citation statements)

References 70 publications

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“…This result correlates with the low genetic variability observed in the Spanish host [3], and it would be in agreement with the hypothesis that GaRV6 could have evolved with its fungal host, which is likely enhanced by the fact that only asexual reproduction has been reported in the Spanish population of the host so far [4,32]. No population data about UvRV4, A1RV1, CthRV, FgV4, and RsRV1 have been reported; , Table S2), 54 % with Rosellinia necatrix victorivirus 1 over 800 nt (U2 , Table S3) and, 55 % with an unknown RNA-dependent RNA polymerase over 328 nt (U3 , Table S4); Mi, dsRNA corresponding to Gremmeniella mitovirus 1 strain 50 (GMV1-50) [6]; GaRV6, dsRNA band corresponding to GaRV6; P, dsRNA corresponding to Gremmeniella abietina RNA virus MS1 strain 35 (GaRV-MS1-35) [7]. (B) Schematic representation of the RdRp segment of GaRV6, including the coding region of the RdRp and the region used for the population study Fig.…”

Section: Discussionmentioning

confidence: 99%

“…For the purpose of describing G. abietina RNA mitovirus 1 [6], we found clones of this possible novel virus. Double-stranded RNA (dsRNA) was purified following the CF11 cellulose affinity [35].…”

Section: Dsrna Extraction and Sequence Determination Of The Rdrp Codimentioning

confidence: 99%

“…Double-stranded RNA (dsRNA) was purified following the CF11 cellulose affinity [35]. Double-stranded (ds) cDNA synthesis and cloning were conducted as described earlier [6,35,36]. The RdRp consensus sequence was constructed based on a total of 15 independent clones sequenced from both directions.…”

Section: Dsrna Extraction and Sequence Determination Of The Rdrp Codimentioning

confidence: 99%

“…Mitoviruses are also present in both biotypes of G. abietina [5,36], but the mitovirus community shows a certain host specificity, as G. abietina mitovirus 1 (GMV1) is more common in biotype A, and G. abietina mitovirus 2 (GMV2) mainly occurs in biotype B. Both of these viruses are hosted by the unique Spanish population [6]. Finally, a taxonomically undescribed virus with some endornavirus-like properties was characterised in biotype B (G. abietina type B RNA virus XL, GaBRV-XL) [38].…”

Section: Introductionmentioning

confidence: 99%

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Description and prevalence of a putative novel mycovirus within the conifer pathogen Gremmeniella abietina

et al. 2015

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The European race of Gremmeniella abietina (Lagerberg) Morelet is the causal agent of stem canker and shoot blight on numerous conifers in Europe and North America. It comprises different species and biotypes in which the presence of mycoviruses has been determined. In this report, we describe the full-length sequence of the RNA-dependent RNA polymerase (RdRp) of a putative novel virus, Gremmeniella abietina RNA virus 6 (GaRV6), with 2165 nt and a GC content of 54.7 %. A BLASTp search using the deduced RdRp amino acid sequence confirmed GaRV6 to be related to members of a still unassigned virus taxon, which includes, e.g., Fusarium graminearum dsRNA mycovirus 4 (FgV-4) and the mutualistic Curvularia thermal tolerance virus (CThTV). The prevalence and genetic diversity of GaRV6 was also studied within the European race of G. abietina. We examined 162 isolates originating from Canada, the Czech Republic, Finland, Italy, Montenegro, Serbia, Spain, Switzerland, Turkey and the United States. According to direct specific reverse transcription (RT) PCR screening based on the RdRp sequence, the virus appears to be present only in Spain, where it is relatively abundant but genetically highly uniform.

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Section: Discussionmentioning

confidence: 99%

Section: Dsrna Extraction and Sequence Determination Of The Rdrp Codimentioning

confidence: 99%

Section: Dsrna Extraction and Sequence Determination Of The Rdrp Codimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Description and prevalence of a putative novel mycovirus within the conifer pathogen Gremmeniella abietina

et al. 2015

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“…All isolates were selected based on previous studies developed in our laboratory in which RT-PCR and sequencing techniques confirmed the presence or absence of mitoviruses (Botella et al, 2012a(Botella et al, , 2012b. The isolates were previously stored in 15% glycerol at -80º C and were reactivated on modified orange serum agar medium (MOS-agar;Müller et al, 1994) before performing the experiment.…”

Section: Fungal Materialsmentioning

confidence: 99%

Effect of putative mitoviruses on in vitro growth of Gremmeniella abietina isolates under different laboratory conditions

2012

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Mitoviruses have been found in several forest pathogens (i.e. Cryphonectria parasitica, Gremmeniella abietina), and because they have been shown to reduce the virulence of host fungi there is a growing interest in studying their use as a biocontrol. This study was carried out to test the effect of temperature (5°C, 15°C, 25°C and 35°C), pH (4, 5, 7 and 9) and osmotic potential (-0.6, -1.2, -1.8 and -2.4 MPa) on the mycelial growth of seven G. abietina isolates under controlled laboratory conditions. Four of the isolates hosted mitoviruses and three of them did not. During the experiment, mycelial growth was recorded every week for a period of 8 weeks. Results showed no differences in growth behavior between mitovirus infected and non-infected isolates when placed under different pH modifications. However, the mitovirus-infected isolates presented larger mycelial growth than the mitovirus-free ones when at the fungi's optimal growing temperature of 15°C. When growing at certain osmotic potentials (-0.6 and -1.8 MPa) a reduction in growth of the mitovirus-infected isolates was observed. The results of this experiment suggest that mycelial growth among non-infected isolates and isolates naturally infected by mitovirus vary under different culture conditions, thus providing further insight into the effects of mitovirus on Gremmeniella abietina isolates.Key words: mitoviruses; Scleroderris canker; in vitro; biological control; Gremmeniella abietina; dsRNA. Resumen Efecto de posibles mitovirus en el crecimiento in vitro de aislados de Gremmeniella abietina bajo diferentes condiciones de laboratorioLos mitovirus son virus exclusivamente fúngicos que han sido aislados de algunos patógenos forestales (i.e. Cryphonectria parasitica, Gremmeniella abietina) y ya que pueden reducir la virulencia del hongo existe un creciente interés por su posible papel como agentes de control biológico. Se ha llevado a cabo un estudio para evaluar el efecto de la temperatura (5°C, 15°C, 25°C y 35°C), el pH (4, 5, 7 y 9) y el potencial osmótico (-0.6, -1.2, -1.8, -2.4 MPa) en el crecimiento micelial de siete aislados de G. abietina bajo condiciones controladas de laboratorio. Cuatro de los aislados albergaban mitovirus y tres de ellos no. Durante el experimento, el crecimiento micelial fue registrado semanalmente hasta completar 8 mediciones. Los aislados infectados con mitovirus presentaron mayor crecimiento micelial que los no infectados a la temperatura de crecimiento óptimo del hongo de 15°C. No se observaron efectos de la presencia de mitovirus entre los aislados infectados y los no infectados en los tratamientos de modificación del pH. Cuando se modificaron los potenciales osmóticos se observó una reducción del crecimiento micelial de los aislados infectados con mitovirus en los potenciales osmóticos de -0.6 y -1.8 MPa. Los resultados de este experimento sugieren que la presencia de los mitovirus afecta al crecimiento micelial del hongo bajo distintas condiciones de laboratorio. Este estudio proporciona un conocimiento más prof...

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Three mitovirus strains infecting a single isolate of Fusarium circinatum are the first putative members of the family Narnaviridae detected in a fungus of the genus Fusarium

Martínez‐Álvarez

Vainio²,

Botella

et al. 2014

Arch Virol

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Fusarium circinatum Nirenberg & O'Donnell (teleomorph = Gibberella circinata) is the causal agent of pitch canker disease of pines. Since 2004 it has been present in Europe, particularly in northern Spain, affecting P. radiata and P. pinaster in plantations and nurseries. The disease has now also spread to other European countries, including France, Italy and Portugal. In this report, we describe three novel members of the genus Mitovirus from a Spanish isolate of F. circinatum: Fusarium circinatum mitovirus 1 (FcMV1), FcMV2-1 and FcMV2-2. Using a mitochondrial translation table, the complete 2419-bp genome of FcMV1 encodes an RNA-dependent RNA polymerase of 731 amino acids (GC-content ca 30 %). The partial genomes of FcMV2-1 and FcMV2-2 (2193 and 1973 bp, respectively) share ca 48 % RdRp sequence similarity at the aa level and might be regarded as conspecific, while FcMV1 is clearly distinct, showing 32-35 % polymerase similarity to the other strains. However, FcMV1 shared 46 % protein-level similarity with Thielaviopsis basicola mitovirus. This is the first study to report viruses in F. circinatum, as well as the first time that mitovirus genome sequences are described from Fusarium spp.

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Occurrence of two different species of mitoviruses in the European race of Gremmeniella abietina var. abietina, both hosted by the genetically unique Spanish population

Cited by 23 publications

References 70 publications

Description and prevalence of a putative novel mycovirus within the conifer pathogen Gremmeniella abietina

Description and prevalence of a putative novel mycovirus within the conifer pathogen Gremmeniella abietina

Effect of putative mitoviruses on in vitro growth of Gremmeniella abietina isolates under different laboratory conditions

Three mitovirus strains infecting a single isolate of Fusarium circinatum are the first putative members of the family Narnaviridae detected in a fungus of the genus Fusarium

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