Occurrence of Vibrio sp. infection in grouper, Epinephelus suillus

Abstract

Vibrio s t , was consistently isolated from grouper, E~'nepbe1~s suillus, with bacterial infection. Fingerlings, w ich were challenged with the bacterium y injection, were highly susceptible. Immersion challenge resulted in 100% mortality within 48 hrs in fish subjected to combination of injury and exposure to the bacterium. Mortality in uninjured fish was observed in the long bath subgroup, but not in the short bath subgroup. These results are correlated with the present practices in the grouper fingerling industry in the Philippines. ZusammenfassungVorkommen von Vibrio-Infektionen in Groupern, Epinephelus suillus

Isolation, identification and pathogenicity of Vibrio harveyi, the causal agent of skin ulcer disease in juvenile hybrid groupers Epinephelus fuscoguttatus × Epinephelus lanceolatus

“…Many studies have confirmed that pathogenic bacteria exhibit stronger virulence in fish with body surface injuries, including Vibrio infection of the orange‐spotted grouper (Lavilla‐Pitogo et al . ), Edwardsiella and Streptococcus in zebrafish (Neely, Pfeifer & Caparon ; Pressley et al . ) and V. anguillarum in Ayu fish (Muroga & De La Cruz ).…”

“…Bacterial suspensions and dilutions were loaded in water tanks (0.2 m long, 0.15 m wide and 0.2 m deep) containing 4 L of sea water at final concentrations of 2.76 9 10 5 and 2.76 9 10 6 colony-forming units (CFU) mL À1 , respectively. Immersion infection was conducted as described by Lavilla-Pitogo et al (1992). A total of 30 healthy juvenile hybrid groupers were randomly divided into five groups (four infected groups and one control group, six fish per group) and challenged by immersion.…”

“…Immersion infection after dermal abrasion was conducted as described by Lavilla-Pitogo et al (1992). A total of 36 healthy juvenile hybrid groupers were randomly divided into six groups (five infected groups and one control group, six fish per group).…”

“…), iridovirus (Chou, Hsu & Peng ; Lü et al . ), various bacteria (mainly vibrios) (Lavilla‐Pitogo, Castillo & De La Cruz ; Lee ; Saeed ; Yii, Yang & Lee ) and parasites (Ruckert et al . ; Mansour et al .…”

“…However, the continual expansion of grouper aquaculture and deterioration of the water environment have led to the emergence of serious diseases affecting cultivated groupers. The main reported pathogens include fish nervous necrosis virus (Chi et al 1997;Lin et al 2001), iridovirus (Chou, Hsu & Peng 1998;L€ u et al 2005), various bacteria (mainly vibrios) (Lavilla-Pitogo, Castillo & De La Cruz 1992;Lee 1995;Saeed 1995;Yii, Yang & Lee 1997) and parasites (Ruckert et al 2009;Mansour et al 2015). As the offspring of brownmarbled and giant groupers, the hybrid grouper, Epinephelus fuscoguttatus (♀) 9 Epinephelus lanceolatus (♂), was successfully bred and cultivated for the first time in Taiwan Province, China, in 2008.…”