Occurrence, pathotypes, and antimicrobial resistance profiles of diarrheagenic Escherichia coli strains in animal source food products from public markets in Mashhad, Iran

Fallah, Neda; Ghaemi, Mehran; Ghazvini, Kiarash; Rad, Mehrnaz; Jamshidi, Abdollah

doi:10.1016/j.foodcont.2020.107640

Cited by 19 publications

(30 citation statements)

References 38 publications

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“…The virulence‐associated genes, such as ST , LT , ipaH , daaD , pCVD and aggR , which were screened as the index of pathotypes, are usually conveyed by plasmids (Fallah et al . 2020), whereas genotyping methods detect the variations of the bacterial genome. Likewise, a weak correlation or dissimilarity of the ESBL profiles and antimicrobial resistance patterns among isolates within clusters reflected the presence of most resistance elements on certain plasmids (Hannah et al .…”

Section: Discussionmentioning

confidence: 99%

“…Details of microbiological and molecular identification and antimicrobial susceptibility testing have been reported previously by Fallah et al . (2020) (the data are not shown). Figures 2 and 3 list the sources and pathotypes of the isolates.…”

Section: Methodsmentioning

confidence: 95%

“…Genomic DNA was extracted from colonies of bacterial cultures using the CinnaPure DNA isolation kit (CinnaGen Co., Tehran, Iran, https://www.cinnagen.com/) according to the manufacturer's protocol (Fallah et al . 2020). Genomic DNA (10–20 ng) was used as a template in 25 µ l volume of standard PCR mixture constituted 2× Red Load Taq Master (manufactured in Jena Bioscience, Germany), RNAse free distilled water and 50 pmol of the primer pair REP 1R and REP 2I for rep‐PCR and 25 pmol of the single primer 1247 for RAPD.…”

Section: Methodsmentioning

confidence: 99%

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Molecular typing and prevalence of extended‐spectrum β‐lactamase genes in diarrhoeagenicEscherichia colistrains isolated from foods and humans in Mashhad, Iran

et al. 2021

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Aims Present study was aimed to determine ESBL‐encoding genes distribution in Diarrhoeagenic Escherichia coli (DEC) isolated from animal‐source food products and human clinical samples in Mashhad, Iran. The strains were also further studied to analyse genotypic diversity and find genetic relationships between them. Methods and Results The number of 85 DEC strains including 52 and 33 strains isolated from 300 food and 520 human stool samples, respectively. Randomly amplified polymorphic DNA (RAPD), and repetitive extragenic palindromic‐PCR (rep‐PCR) typing methods were used to track their genetic relationships. The ESBL‐encoding genes prevalence was approximately 70% in both groups of isolates. The blaTEM, blaCTX‐M and blaSHV were prevalent in 67·1, 20 and 10·6% of isolates, respectively. The ESBL‐positives showed significantly higher resistance rates to gentamicin, co‐trimoxazole, tetracycline, aztreonam and chloramphenicol (P < 0·05). Fingerprinting patterns‐based dendrograms divided DEC strains into separate clusters irrespective of their sources and pathotypes. In typing field, rep‐PCR provided more discriminatory power (Simpson's index of diversity (SID) = 0·925) than RAPD (SID = 0·812). Conclusion Molecular similarity between certain animal‐sourced food products and clinical sample strains supported food‐borne transmission routes for genotypic elements such as ESBL‐encoding genes. Significance and Impact of the Study Findings emphasize the importance of resistance issues, the need to improve treatment guidelines and routine surveillance of hygienic measures during food processing.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 95%

Section: Methodsmentioning

confidence: 99%

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Molecular typing and prevalence of extended‐spectrum β‐lactamase genes in diarrhoeagenicEscherichia colistrains isolated from foods and humans in Mashhad, Iran

et al. 2021

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“…Ombarak et al (2018) found that (1.4%) of E.coli isolates from raw milk and raw milk cheese in Egypt were resistant to ciprofloxacin. Also Fallah et al (2021) recorded very low resistance (12.1%) was observed for ciprofloxacin in the STEC group isolated from food products. Hassan and Elmalt (2008) revealed that most frequent resistance of E.coli isolates from raw milk and kariesh cheese was observed to the following antimicrobials: nalidixic acid (42.9%), ampicillin (32.7%), tetracycline (22.4%) and ciprofloxacin (4.1%).…”

Section: Serological Identification Of Ecoli Was Carried Out Bymentioning

confidence: 91%

http://www.plantarchives.org/SPECIAL%20ISSUE%2021-1/429%20(2636-2640).pdf

Mohammed¹,

El-Said²,

AbdElAal³

et al. 2020

PLANT ARCHIVES

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In the present study, Fifty random samples of each small-scale (plain yoghurt and kariesh cheese) were collected randomly from different dairy shops and markets in Zagazig city for isolation and identification of Escherichia coli which is considered a good indicator of faecal contamination and a major cause of food poisoning. Identification was done microscopically and biochemically by different biochemical tests (IMVIC).The incidence of E. coli in yoghurt and kariesh cheese samples were 36% and 50%, respectively. Also, the serological identification of E. coli isolates revealed that O26 is the most predominant serogroup by percentagesof27.77% and 28% in both yoghurt and kariesh cheese samples, respectively. Because of its rising resistance to antibiotics, E.coli represents a real threat to human health. Antimicrobial susceptibility testing (AST) was done by disc diffusion method against 10 antimicrobials and the results revealed that E. coli isolates were highly resistant to amoxicillin-clavulanate, ampicillin, Cefotaxime and Ceftazidime, and were highly sensitive to chloramphenicol, Ciprofloxacin and tetracycline. In addition, 83.72%of E. coli isolates showed multi drug resistance (MDR).Bacterial adhesion to food surfaces and the formation of biofilmisa source of food contamination that has an impact on food safety and industry. Micro titer plate assay was used for testing biofilm formation and revealed that 69.77% of E. coli isolates were non-biofilm producers, 9.30% were weak biofilm producers, 20.93%were moderate biofilm producers and none of isolates was strong biofilm producers. Virulence genes of E. coli isolates were identified and characterized by a multiplex PCR assay. The results showed that among the target genes, stx1was most frequently detected by a percentage of81.82%

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“…Escherichia coli and Salmonella enterica ser. Typhimurium are among the main Gram-negative pathogens causing gastrointestinal (GI) infections in humans and pigs [ 9 , 10 , 11 ]. Furthermore, the former is commonly associated to the latter as both bacteria are zoonotic, and foodborne transmission with pork products represents a significant proportion of human cases [ 10 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Protective Effects of Grape Seed Oligomeric Proanthocyanidins in IPEC-J2–Escherichia coli/Salmonella Typhimurium Co-Culture

et al. 2022

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Intestinal epithelium provides the largest barrier protecting mammalian species from harmful external factors; however, it can be severely compromised by the presence of bacteria in the gastrointestinal (GI) tract. Antibiotics have been widely used for the prevention and treatment of GI bacterial infections, leading to antimicrobial resistance in human and veterinary medicine alike. In order to decrease antibiotic usage, natural substances, such as flavonoids, are investigated to be used as antibiotic alternatives. Proanthocyanidins (PAs) are potential candidates for this purpose owing to their various beneficial effects in humans and animals. In this study, protective effects of grape seed oligomeric proanthocyanidins (GSOPs) were tested in IPEC-J2 porcine intestinal epithelial cells infected with Escherichia coli and Salmonella enterica ser. Typhimurium of swine origin. GSOPs were able to alleviate oxidative stress, inflammation and barrier integrity disruption inflicted by bacteria in the co-culture. Furthermore, GSOPs could decrease the adhesion of both bacteria to IPEC-J2 cells. Based on these observations, GSOPs seem to be promising candidates for the prevention and treatment of gastrointestinal bacterial infections.

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Occurrence, pathotypes, and antimicrobial resistance profiles of diarrheagenic Escherichia coli strains in animal source food products from public markets in Mashhad, Iran

Cited by 19 publications

References 38 publications

Molecular typing and prevalence of extended‐spectrum β‐lactamase genes in diarrhoeagenicEscherichia colistrains isolated from foods and humans in Mashhad, Iran

Molecular typing and prevalence of extended‐spectrum β‐lactamase genes in diarrhoeagenicEscherichia colistrains isolated from foods and humans in Mashhad, Iran

http://www.plantarchives.org/SPECIAL%20ISSUE%2021-1/429%20(2636-2640).pdf

Protective Effects of Grape Seed Oligomeric Proanthocyanidins in IPEC-J2–Escherichia coli/Salmonella Typhimurium Co-Culture

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