Occurrences and Characterization of Antibiotic-Resistant Bacteria and Genetic Determinants of Hospital Wastewater in a Tropical Country

Le, Thai-Hoang; Ng, Charmaine; Chen, Hongjie; Xin, Yi; Koh, Tse Hsien; Barkham, Timothy; Zhou, Zhi; Gin, Karina Yew‐Hoong

doi:10.1128/aac.01556-16

Cited by 102 publications

(60 citation statements)

References 45 publications

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“…Here, it was found that E. coli had the highest resistance to the all drugs tested (100%), this could be attributed to the abundance nature of E. coli in environment and its easy adaptability. Similar trend of high resistance pattern of E. coli from hospital sewage was reported [30,33,[43][44]. In this present study, least resistance by Morganella morganii (20%) was observed.…”

Section: Discussionsupporting

confidence: 90%

“…By implication, discharging such sewage get into large water receiving bodies without proper treatment could further allow dissemination of bacterial resistance isolates. High bacterial counts from hospital sewage and effluents are also reported [33]. Lein et.…”

Section: Discussionmentioning

confidence: 90%

“…Profiles of bacterial population in different environments have been reported worldwide. In addition, the resistance profiles of such bacteria have been studied [30][31][32][33][34]. This study focuses to investigate the members of gram-negative bacteria from hospital sewage and their resistance profile to the most commonly used antibiotics in the study area.…”

Section: Discussionmentioning

confidence: 99%

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Detection of Multidrug-Resistance Gram-Negative Bacteria from Hospital Sewage in North East, Nigeria

Mustapha¹

2019

FEM

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Antibiotic resistance is on the verge of becoming top killer globally if left unattended in few decades to come.Much focus has been on clinical use and misuse of antibiotics and non-therapeutic applications in agriculture are blamed for the emergence of resistance. However, the rising incident of environmental spread of antibiotic is a major public health concern. The purpose of this study is to investigate the occurrence of gram-negative bacteria from hospitals sewage and evaluate the multi-drug resistant pattern of the isolates in the study area. Sample from five government's hospitals in Damaturu, Northern Nigeria were collected. The bacteria were quantified using pour plating method; colonies were counted and further characterized by morphological and biochemical characteristics using standard microbiological techniques. Antibiotic sensitivity testing was determined by Kirby-Baur disc diffusion method. A total of 1377 gram-negative isolates were identified;

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

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Detection of Multidrug-Resistance Gram-Negative Bacteria from Hospital Sewage in North East, Nigeria

Mustapha¹

2019

FEM

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“…The ranking framework accurately identified 95% of all well-known high risk ARG families as Rank I ARGs and expanded the list by 43 unreported Rank I ARG families. We compared our results against a list of 38 ARG families that were reported to have high clinical concern (to cause treatment failure of healthcare associated infections and/or have been wide-spread phylogenetically and geographically) by the WHO and by literature review (highlighted in purple) 17,19,20,22,[38][39][40][41][42][43][44][45][46][47] (Figure 3). For example, we included extended-spectrum beta-lactamases (ESBL) ARGs (i.e., TEM, SHV, and CTX-M), that recently caused the death of fecal microbiota transplantation (FMT) recipients.…”

Section: Test Figures 1b and S2amentioning

confidence: 99%

Choosing Your Battles: Which Resistance Genes Warrant Global Action?

Zhang

Yin

et al. 2019

Preprint

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The increasing accumulation of antibiotic resistance genes (ARGs) in pathogens poses a severe threat to the treatment of bacterial infections. However, not all ARGs do not pose the same threats to human health. Here, we present a framework to rank the risk of ARGs based on three factors: "anthropogenic enrichment", "mobility", and "host pathogenicity". The framework is informed by all available bacterial genomes (55,000), plasmids (16,000), integrons (3,000), and 850 metagenomes covering diverse global eco-habitats. The framework prioritizes 3% of all known ARGs in Rank I (the most at risk of dissemination amongst pathogens) and 0.3% of ARGs in Rank II (high potential emergence of new resistance in pathogens). We further validated the framework using a list of 38 ARG families previously identified as high risk by the World Health Organization and published literature, and found that 36 of them were properly identified as top risk (Rank I) in our approach. Furthermore, we identified 43 unreported Rank I ARG families that should be prioritized for public health interventions. Within the same gene family, homologous genes pose different risks, host range, and ecological distributions, indicating the need for high resolution surveillance into their sequence variants. Finally, to help strategize the policy interventions, we studied the impact of industrialization on high risk ARGs in 1,120 human gut microbiome metagenomes of 36 diverse global populations. Our findings suggest that current policies on controlling the clinical antimicrobial consumptions could effectively control Rank I, while greater antibiotic stewardship in veterinary settings could help control Rank II. Overall, our framework offered a straightforward evaluation of the risk posed by ARGs, and prioritized a shortlist of current and emerging threats for global action to fight ARGs.

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“…Besides the presence of viable NDM-positive bacteria in wastewater, blaNDM-1 genes were also shown to be present at significant numbers in municipal wastewaters, which include wastewater discharged from hospitals. Untreated hospital wastewater from two hospitals in Singapore contained 2.29 x 10 6 gene copies/mL of blaNDM and 4.08 x 10 7 , 1.25 x 10 6 , and 6.19 x 10 5 gene copies/mL, of genes blaKPC, blaCTX-M, and blaSHV, respectively (Le et al 2016). Another study that monitored blaNDM-1 numbers in raw wastewater entering a WWTP in Saudi Arabia reported 3.4 x 10 4 ± 2.3 x 10 4 copies/m 3 (Mantilla-Calderon et al 2016).…”

Section: New Delhi Metallo-beta-lactamase In Wastewatermentioning

confidence: 99%

Potential Dissemination of ARB and ARGs into Soil Through the Use of Treated Wastewater for Agricultural Irrigation: Is It a True Cause for Concern?

Aljassim

Hong

2017

Soil Biology

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Resistance to antibiotics is increasingly being recognized as an emerging contaminant posing great risks to effective treatment of infections and to public health. Pristine soils or even soils that predate the antibiotic era naturally contain ARB and ARG. This book chapter explores the native resistome of soils, and collates information on whether soil perturbation through wastewater reuse can lead to accumulation of ARB and ARGs in agricultural soils. Special emphasis was given to ARGs, particularly the blaNDM gene that confers resistance against carbapenem. The fate and persistence of these emerging ARGs have not been studied in depth, however this book chapter reviews available information on other ARGs to gain insight into the possibility of horizontal gene transfer events in wastewater-irrigated soils and plant surfaces and tissues. Lastly, this book chapter visits solar irradiation and bacteriophage treatment as intervention options to limit dissemination of emerging contaminant threats.

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Occurrences and Characterization of Antibiotic-Resistant Bacteria and Genetic Determinants of Hospital Wastewater in a Tropical Country

Cited by 102 publications

References 45 publications

Detection of Multidrug-Resistance Gram-Negative Bacteria from Hospital Sewage in North East, Nigeria

Detection of Multidrug-Resistance Gram-Negative Bacteria from Hospital Sewage in North East, Nigeria

Choosing Your Battles: Which Resistance Genes Warrant Global Action?

Potential Dissemination of ARB and ARGs into Soil Through the Use of Treated Wastewater for Agricultural Irrigation: Is It a True Cause for Concern?

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