Occurring of<i>In Vitro</i>Functional Vasculogenic Pericytes from Human Circulating Early Endothelial Precursor Cell Culture

Cantoni, Silvia; Bianchi, Francesca; Galletti, Martina; Olivi, Elena; Alviano, Francesco; Galiè, Nazzareno; Ventura, Carlo

doi:10.1155/2015/943671

Cited by 9 publications

(7 citation statements)

References 43 publications

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“…We used flow cytometry to examine the purity of MEC. The expression rate of immunophenotype vWF was 81.39%, CD31 expression rate was 45.8%, CD34 expression rate was 57.48% and CD45 expression rate was 0.17% ( Figure 1E), which is in line with the phenotypic characteristics of MEC [14][15][16][17].…”

Section: The Morphology Of Microvascular Endothelial Cellsupporting

confidence: 76%

Role of microvascular endothelial cells on proliferation, migration and adhesion of hematopoietic stem cells

et al. 2020

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Background: The present study investigated the effects of microvascular endothelial cells (MECs) on the chemotaxis, adhesion and proliferation of bone marrow hematopoietic stem cells (HSCs) ex vivo. Methods and Results: MECs were collected from the lung tissue of C57BL/6 mice, and HSCs were isolated with immunomagnetic beads from bone marrow of GFP mice. MECs and HSCs were co-cultured with or without having direct cell–cell contact in Transwell device for the measurement of chemotaxis and adhesion of MECs to HSCs. Experimental results indicate that the penetration rate of HSCs from the Transwell upper chamber to lower chamber in ‘co-culture’ group was significantly higher than that of ‘HSC single culture’ group. Also, the HSCs in co-culture group were all adherent at 24 h, and the co-culture group with direct cell–cell contact had highest proliferation rate. The HSC number was positively correlated with vascular endothelial growth factor (VEGF) and stromal cell-derived factor-1 (SDF-1) levels in supernatants of the culture. Conclusions: Our study reports that MECs enhance the chemotaxis, adhesion and proliferation of HSCs, which might be related to cytokines SDF-1 and VEGF secreted by MECs, and thus MECs enhance the HSC proliferation through cell–cell contact. The present study revealed the effect of MECs on HSCs, and provided a basis and direction for effective expansion of HSCs ex vivo.

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Section: The Morphology Of Microvascular Endothelial Cellsupporting

confidence: 76%

Role of microvascular endothelial cells on proliferation, migration and adhesion of hematopoietic stem cells

et al. 2020

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“…They, hence, represent two early stem cell markers [Iancu et al, 2015]. It has been proven in in vitro conditions that early endothelial progenitor cells can be a source of pericytes/mesenchymal-like cells that have a mesenchymal stem cell phenotype and exhibit both the expression of pluripotent genes, such as nanog, and a remarkable vasculogenic potential [Cantoni et al, 2015]. This appears to quite convincingly indicate that TC-like cells belong to a continuum ranging from endothelial progenitors to newly formed ECs.…”

Section: Cardiac Ecs and Tcsmentioning

confidence: 99%

Myocardial Telocyte-Like Cells: A Review Including New Evidence

Iancu

Rusu

Mogoantă

et al. 2018

Cells Tissues Organs

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Telocytes (TCs) are a controversial cell type characterized by the presence of a particular kind of prolongations, known as telopodes, which are long, thin, and moniliform. A number of attempts has been made to establish the molecular phenotype of cardiac TCs (i.e., expression of c-kit, CD34, vimentin, PDGRFα, PDGRFβ, etc.). We designed an immunohistochemical study involving cardiac tissue samples obtained from 10 cadavers with the aim of determining whether there are TC-like interstitial cells that populate the interstitial space other than the mural microvascular cells. We applied the markers for CD31, CD34, PDGRFα, CD117/c-kit, and α-smooth muscle actin (α-SMA). We found that, in relation to two-dimensional cuts, the endothelial tubes could be misidentified as TC-like cells, the difference being the positive identification of endothelial lumina. Moreover, we found that cardiac pericytes express PDGRFα, CD117/c-kit, and α-SMA, and that they could also be misidentified as TCs when using light microscopy. We reviewed the respective values of the previously identified markers for achieving a clear-cut identification of cardiac TCs, highlighting the critical lack of specificity.

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“…Embryonic sources of pericytes include neuro-ectoderm-derived neural crest cells that are thought to give rise to pericytes in the brain and central nervous system ( Figure 1 B), whereas pericytes in other organs have been postulated to originate from the mesoderm-derived MSPCs ( Figure 1 A). Postnatal sources of pericytes include mesoderm-derived circulating BM-MSPCs, transdifferentiated ECs, CD146 + EPCs, mesothelial cells that have undergone EMT, or as yet undetermined “other sources” [ 47 , 138 , 142 , 214 , 215 ] (reviewed by Hill et al [ 136 ]). Similarly, the source of (myo)fibroblasts remains controversial, and they have been reported to develop from epithelial cells via EMT, from ECs via EndoMT, from BM-MSPCs, from peripheral blood pluripotent circulating progenitor cells, and from pericytes (recently reviewed in [ 216 , 217 ]).…”

Section: Mspcs: Cell Of Originmentioning

confidence: 99%

Mesenchymal Stem and Progenitor Cells in Normal and Dysplastic Hematopoiesis—Masters of Survival and Clonality?

Pleyer

Valent

Greil

2016

IJMS

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Myelodysplastic syndromes (MDS) are malignant hematopoietic stem cell disorders that have the capacity to progress to acute myeloid leukemia (AML). Accumulating evidence suggests that the altered bone marrow (BM) microenvironment in general, and in particular the components of the stem cell niche, including mesenchymal stem cells (MSCs) and their progeny, play a pivotal role in the evolution and propagation of MDS. We here present an overview of the role of MSCs in the pathogenesis of MDS, with emphasis on cellular interactions in the BM microenvironment and related stem cell niche concepts. MSCs have potent immunomodulatory capacities and communicate with diverse immune cells, but also interact with various other cellular components of the microenvironment as well as with normal and leukemic stem and progenitor cells. Moreover, compared to normal MSCs, MSCs in MDS and AML often exhibit altered gene expression profiles, an aberrant phenotype, and abnormal functional properties. These alterations supposedly contribute to the “reprogramming” of the stem cell niche into a disease-permissive microenvironment where an altered immune system, abnormal stem cell niche interactions, and an impaired growth control lead to disease progression. The current article also reviews molecular targets that play a role in such cellular interactions and possibilities to interfere with abnormal stem cell niche interactions by using specific targeted drugs.

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Occurring ofIn VitroFunctional Vasculogenic Pericytes from Human Circulating Early Endothelial Precursor Cell Culture

Cited by 9 publications

References 43 publications

Role of microvascular endothelial cells on proliferation, migration and adhesion of hematopoietic stem cells

Role of microvascular endothelial cells on proliferation, migration and adhesion of hematopoietic stem cells

Myocardial Telocyte-Like Cells: A Review Including New Evidence

Mesenchymal Stem and Progenitor Cells in Normal and Dysplastic Hematopoiesis—Masters of Survival and Clonality?

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