Ocean Acidification and Warming Lead to Increased Growth and Altered Chloroplast Morphology in the Thermo-Tolerant Alga Symbiochlorum hainanensis

Gong, Sanqiang; Jin, Xuejie; Xiao, Yilin; Li, Zhiyong

doi:10.3389/fpls.2020.585202

Cited by 16 publications

(8 citation statements)

References 54 publications

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“…RNA samples with high purity (OD260/280 between 1.9 and 2.1) and high integrity [RNA integrity number (RIN) >8.0] were used for further cDNA library construction. The sequencing were performed according to our previous study ( 53 ).…”

Section: Methodsmentioning

confidence: 99%

Unfolding the secrets of microbiome (Symbiodiniaceae and bacteria) in cold-water coral

Gong,

Liang,

Jin

et al. 2023

Microbiol Spectr

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Recent deep-ocean exploration has uncovered a variety of cold-water coral (CWC) ecosystems around the world ocean, but it remains unclear how microbiome is associated with these corals at a molecular levels. This study utilized metabarcoding, tissue section observation, and metatranscriptomes to investigate the microbiome (Symbiodiniaceae and bacteria) of CWC species ( Narella versluys i, Heterogorgia uatumani, and Muriceides sp.) from depths ranging from 260 m to 370 m. Warm-water coral (WWC) species ( Acropora pruinosa , Pocillopora damicorni s, and Galaxea fascicularis ) were used as control groups. Results revealed that CWC host diverse bacteria and Symbiodiniaceae cells were observed in endoderm of CWC tissues. Several new candidate bacterial phyla were found in both CWC and WWC, including Coralsanbacteria, Coralqiangbacteria, Coralgsqaceae, Coralgongineae, etc. Both the 16S rRNA gene sequencing and metatranscriptomes revealed that Actinobacteria and Proteobacteria were abundant bacterial phyla in CWC. At the gene transcription level, the CWC-associated Symbiodiniaceae community showed a low-level transcription of genes involved in photosynthesis, CO 2 fixation, glycolysis, citric acid cycle, while bacteria associated with CWC exhibited a high-level transcription of genes for carbon fixation via the Wood-Lijungdahl pathway, short chain fatty acids production, nitrogen, and sulfur cycles. IMPORTANCE This study shed new light on the functions of both Symbiodiniaceae and bacteria in cold-water coral (CWC). The results demonstrated that Symbiodiniaceae can survive and actively transcribe genes in CWC, suggesting a possible symbiotic or parasitic relationship with the host. This study also revealed complete non-photosynthetic CO 2 fixation pathway of bacteria in CWC, as well as their roles in short chain fatty acids production and assimilation of host-derived organic nitrogen and sulfur. These findings highlight the important role of bacteria in the carbon, nitrogen sulfur cycles in CWC, which were possibly crucial for CWC survival in in deep-water environments.

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Section: Methodsmentioning

confidence: 99%

Unfolding the secrets of microbiome (Symbiodiniaceae and bacteria) in cold-water coral

Gong,

Liang,

Jin

et al. 2023

Microbiol Spectr

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“…RNA samples with high purity (OD260/280 between 1.9 and 2.1) and high integrity [RNA integrity number (RIN) > 8.5] were used for further cDNA library construction. cDNA library construction and the sequencing and quality control of raw sequences were performed according to our previous study (Gong et al 2020b ).…”

Section: Methodsmentioning

confidence: 99%

Linking coral fluorescence phenotypes to thermal bleaching in the reef-building Galaxea fascicularis from the northern South China Sea

Gong,

Liang,

et al. 2023

Mar Life Sci Technol

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Coral fluorescence phenotypes have been suggested as an adaptation to a broad range of environmental conditions, yet the mechanisms linking thermal bleaching tolerance in reef-building coral populations, associated with fluorescence phenotypes due to GFP-like proteins, remains unclear. In this study, the relationship between the thermal sensitivity and phenotypic plasticity of corals was investigated using two phenotypes of Galaxea fascicularis, green and brown. The results reveal that brown G. fascicularis was more susceptible to bleaching than green G. fascicularis when exposed to a higher growth temperature of 32 °C. Both phenotypes of G. fascicularis were associated with the thermotolerant Symbiodiniaceae symbiont, Durusdinium trenchii. However, the brown G. fascicularis showed a significant decrease in Symbiodiniaceae cell density and a significant increase in pathogenic bacteria abundance when the growth temperature was raised from 29 to 32 °C. The physiological traits and transcriptomic profiles of Symbiodiniaceae were not notably affected, but there were differences in the transcriptional levels of certain genes between the two phenotype hosts of G. fascicularis. Under heat stress of 32 °C, the gene encoding green fluorescent protein (GFP)-like and chromosome-associated proteins, as well as genes related to oxidative phosphorylation, cell growth and death showed lower transcriptional levels in the brown G. fascicularis compared to the green G. fascicularis. Overall, the results demonstrate that the green form of G. fascicularis is better able to tolerate ocean warming and defend against pathogenic bacteria, likely due to higher gene transcription levels and defense ability.

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“…RNA samples with high purity (OD260/280 between 1.8 and 2.2) and high integrity (RNA integrity number (RIN) > 7.5) were used for further cDNA library construction. cDNA library construction and the sequencing and quality control of raw sequences were performed according to our previous study (Gong et al, 2020). The raw sequence data sets (a total of 12 sequencing libraries, the statistical information of RNA-seq data sets were supplied in Supplementary Table 1) produced in this study were deposited in the Sequence Read Archive (PRJNA699996) of the NCBI (see text footnote 1).…”

Section: Rna Sequencing and Gene Transcriptionmentioning

confidence: 99%

Photobleaching and Recovery of Symbiodiniaceae Effrenium voratum SCS01 Reveals Life Form Transformation Under Thermal Stress

Gong

Jin

et al. 2021

Front. Mar. Sci.

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Dinoflagellates in the family Symbiodiniaceae contain a number of species and play an important role in the establishment of coral reef ecosystems in oligotrophic marine waters. Effrenium voratum is likely an exclusively free-living and heterotrophic species of Symbiodiniaceae. How this species responds and acclimates to warming is largely unknown. The present study experimentally established the phenotypic landscapes related to the photobleaching and recovery processes of Effrenium voratum SCS01 following thermal stress. We found that thermal stress bleached the plastids of E. voratum SCS01 and caused the cells to become lighter in color. Thereafter, the bleached cells recovered rapidly when they returned to the optimal temperature. The dominant life form of E. voratum SCS01 shifted from mastigote cells to coccoid cells then returned to mastigote cells. Transcriptome analysis revealed that the photobleaching of E. voratum SCS01 was due to increased degradation and decreased biosynthesis of photosynthetic pigments. The thermally induced life form changes were related to the downregulation of genes for cell motility. Our results revealed the mechanism of photobleaching in E. voratum SCS01 and indicated life form transformation as a newly identified survival strategy of Effrenium voratum SCS01 under thermal stress.

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Ocean Acidification and Warming Lead to Increased Growth and Altered Chloroplast Morphology in the Thermo-Tolerant Alga Symbiochlorum hainanensis

Cited by 16 publications

References 54 publications

Unfolding the secrets of microbiome (Symbiodiniaceae and bacteria) in cold-water coral

Unfolding the secrets of microbiome (Symbiodiniaceae and bacteria) in cold-water coral

Linking coral fluorescence phenotypes to thermal bleaching in the reef-building Galaxea fascicularis from the northern South China Sea

Photobleaching and Recovery of Symbiodiniaceae Effrenium voratum SCS01 Reveals Life Form Transformation Under Thermal Stress

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