Oceanobacillus endoradicis sp. nov., an endophytic bacterial species isolated from the root of Paris polyphylla Smith var. yunnanensis

Abstract

A bacterial strain, py1294(T), isolated from a root of Paris polyphylla Smith var. yunnanensis collected from Yunnan province, southwest China, was characterised by using a polyphasic approach to clarify its taxonomic position. Strain py1294(T) was found to be Gram-positive, aerobic, spore-forming, peritrichous flagella and rod shaped. Growth was found to occur in the presence of 0-8 % (w/v) NaCl (optimum 1-3 %), at pH 6.5-9.5 (optimum 8.0) and at 10-42 °C (optimum 30 °C). The major cellular fatty acids were identified as anteiso-C15:0, anteiso-C17:0, iso-C16:0 and iso-C14:0. The predominant quinone was identified as MK-7 and a minor amount of MK-6 was detected. The diagnostic polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The cell wall peptidoglycan was found to contain meso-diaminopimelic acid. Phylogenetic analysis of the 16S rRNA gene sequence showed that strain py1294(T) forms a well-supported clade with Oceanobacillus damuensis PT-20(T) (97.9 % sequence similarity) within the genus Oceanobacillus, although it also shares a high sequence similarity with Ornithinibacillus contaminans (97.5 %). Crucially, the DNA-DNA relatedness value between strain py1294(T) and O. damuensis PT-20(T) was 29.7 ± 3.2 %. The G+C content was determined to be 42.3 mol%. On the basis of the phylogenetic and phenotypic data, a novel species Oceanobacillus endoradicis sp. nov. is proposed, with py1294(T) (=DSM 100726(T) = KCTC 33731(T)) as the type strain.

Distinguishing nutrient‐dependent plant driven bacterial colonization patterns in alfalfa

“…A. thaliana and rice) (Vorholt, ). Six of the eight genera represented in our synthetic community were previously identified as M. sativa endophytes, and the other two, Williamsia and Oceanobacillus , were previously isolated from the phyllosphere and root endosphere of legume and non‐leguminous plants (Kaewkla and Franco, ; Stiefel et al ., ; Horn et al ., ; Yang et al ., ). Thus, our synthetic community is composed of M. sativa relevant bacteria.…”

Endophytes from Halotolerant Plants Aimed to Overcome Salinity and Draught

“…It was reported about the isolation of new bacterial species Oceanobacillus endoradicis endophyte from the root of Paris polyphylla Smith var. yunnanensis [ 42 ]. The genera Pantoea , Alcaligenes , and Myroides are also quite common among endophytic bacteria.…”

Genetic Diversity, Chemical Components, and Property of Biomass Paris polyphylla var. yunnanensis

“…With the rapid development of molecular biology theory, molecular marker technology will be developed with fast analysis speed, low cost, and more information (Ma et al, 2021a,b;Xu et al, 2020;Xu R. et al, 2021). Yang et al (2016) reported the bacterial strain, py1294(T), isolated from a root of P. polyphylla var. yunnanensis by using a polyphasic approach to clarify its taxonomic position.…”