Ochnaflavone, a Natural Biflavonoid, Induces Cell Cycle Arrest and Apoptosis in HCT-15 Human Colon Cancer Cells

Kang, You-Jin; Min, Hye‐Young; Hong, Ji‐Young; Kim, Yeong Shik; Kang, Sam Sik; Lee, Sang Kook

doi:10.4062/biomolther.2009.17.3.282

Cited by 8 publications

(6 citation statements)

References 27 publications

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“…Many flavonoids are shown to have antioxidant activity, coronary heart disease prevention, anti-inflammation, oestrogenic activity, anticancer activity, and other biological activities [ 41 – 43 ]. As such research progresses, potential application of flavonoids in either foods or pharmaceutical supplements will expand.…”

Section: Discussionmentioning

confidence: 99%

Butin (7,3′,4′-Trihydroxydihydroflavone) Reduces Oxidative Stress-Induced Cell Death via Inhibition of the Mitochondria-Dependent Apoptotic Pathway

Zhang

Lee

Piao

et al. 2011

IJMS

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Recently, we demonstrated that butin (7,3′,4′-trihydroxydihydroflavone) protected cells against hydrogen peroxide (H2O2)-induced apoptosis by: (1) scavenging reactive oxygen species (ROS), activating antioxidant enzymes such superoxide dismutase and catalase; (2) decreasing oxidative stress-induced 8-hydroxy-2′-deoxyguanosine levels via activation of oxoguanine glycosylase 1, and (3), reducing oxidative stress-induced mitochondrial dysfunction. The objective of this study was to determine the cytoprotective effects of butin on oxidative stress-induced mitochondria-dependent apoptosis, and possible mechanisms involved. Butin significantly reduced H2O2-induced loss of mitochondrial membrane potential as determined by confocal image analysis and flow cytometry, alterations in Bcl-2 family proteins such as decrease in Bcl-2 expression and increase in Bax and phospho Bcl-2 expression, release of cytochrome c from mitochondria into the cytosol and activation of caspases 9 and 3. Furthermore, the anti-apoptotic effect of butin was exerted via inhibition of mitogen-activated protein kinase kinase-4, c-Jun NH2-terminal kinase (JNK) and activator protein-1 cascades induced by H2O2 treatment. Finally, butin exhibited protective effects against H2O2-induced apoptosis, as demonstrated by decreased apoptotic bodies, sub-G1 hypodiploid cells and DNA fragmentation. Taken together, the protective effects of butin against H2O2-induced apoptosis were exerted via blockade of membrane potential depolarization, inhibition of the JNK pathway and mitochondria-involved caspase-dependent apoptotic pathway.

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Section: Discussionmentioning

confidence: 99%

Butin (7,3′,4′-Trihydroxydihydroflavone) Reduces Oxidative Stress-Induced Cell Death via Inhibition of the Mitochondria-Dependent Apoptotic Pathway

Zhang

Lee

Piao

et al. 2011

IJMS

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“…It is considered as the taxonomic marker for the genus Ochna [13]. A wide range of activities have been reported for 1 , amongst them prominent anticancer [14–15], anti-inflammatory and atherogenic activity [16–17]. The activity associated with 1 prompted us to develop an efficient synthesis of this compound.…”

Section: Introductionmentioning

confidence: 99%

Total synthesis of ochnaflavone

Ndoile

Heerden

2013

Beilstein J. Org. Chem.

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SummaryThe first total syntheses of ochnaflavone, an asymmetric biflavone consisting of apigenin and luteolin moieties, and the permethyl ether of 2,3,2'',3''-tetrahydroochnaflavone have been achieved. The key steps in the synthesis of ochnaflavone were the formation of a diaryl ether and ring cyclization of an ether-linked dimeric chalcone to assemble the two flavone nuclei. Optimal experimental conditions for the oxidative cyclization to form ochnaflavone were established.

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“…Furthermore, OC's suppression activity remained significant for up to 48 h after incubation, whereas the inhibitory effects of apigenin gradually diminished over time. It is worth mentioning that quercetin and apigenin solely inhibited both OC and LPS‐induced lymphocyte proliferation, whereas OC exhibited irreversible inhibition of proliferation in both cell types (Kang et al., 2009; Zhang et al., 2014). The ‐C‐NH‐C‐ and ‐C‐S‐C‐ analogs of OC have been successfully synthesized.…”

Section: Molecular Mechanisms Of Action Of Ochnaflavonementioning

confidence: 99%

Advances in resources, biosynthesis pathway, bioavailability, bioactivity, and pharmacology of ochnaflavone

Lin,

Ganesan,

Vargas‐De‐La‐Cruz

et al. 2024

Future Postharvest and Food

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Ochnaflavone is a naturally occurring biflavonoid mainly isolated from Ochna integerrima, manifests health benefits encompassing antidiabetic, anticancer, anti‐cardiovascular, and anti‐inflammatory activities. However, most bioactivity research has focused on in vitro experiments, rather than in vivo disease models, toxicological assessments, and human clinical trials. Moreover, a comprehensive review of the pharmacological aspects of ochnaflavone is conspicuously lacking. Thus, this review provides a concise and comprehensive summary of existing knowledge on the chemical structure, plant origin, physical properties, biotransformations, and multifaceted biological activities of ochnaflavone along with an in‐depth exploration of the complex molecular mechanisms behind these activities, including signaling pathways and gene expression regulation, with the aim of promoting future theoretical needs for ochnaflavone in clinical trials and providing comprehensive insights into the research and application of this valuable natural compound.

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Ochnaflavone, a Natural Biflavonoid, Induces Cell Cycle Arrest and Apoptosis in HCT-15 Human Colon Cancer Cells

Cited by 8 publications

References 27 publications

Butin (7,3′,4′-Trihydroxydihydroflavone) Reduces Oxidative Stress-Induced Cell Death via Inhibition of the Mitochondria-Dependent Apoptotic Pathway

Butin (7,3′,4′-Trihydroxydihydroflavone) Reduces Oxidative Stress-Induced Cell Death via Inhibition of the Mitochondria-Dependent Apoptotic Pathway

Total synthesis of ochnaflavone

Advances in resources, biosynthesis pathway, bioavailability, bioactivity, and pharmacology of ochnaflavone

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