Ochrobactrum pseudintermedium sp. nov., a novel member of the family Brucellaceae, isolated from human clinical samples

Teyssier, Corinne; Marchandin, Hélène; Jean-Pierre, Hélène; Masnou, Agnès; Dusart, G.; Jumas‐Bilak, Estelle

doi:10.1099/ijs.0.64416-0

Cited by 56 publications

(42 citation statements)

References 26 publications

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“…nov. E6 T ; 2, Cucumibacter; 3, Devosia; 4, Ahrensia; 5, Ochrobactrum. Data were taken from this study, Hwang & Cho (2008), Ryu et al (2008), Vanparys et al (2005), Rivas et al (2003), Nakagawa et al (1996), Rüger & Höfle (1992), Teyssier et al (2007), Uchino et al (1998), Tripathi et al (2006), Zurdo-Piñeiro et al (2007) and Trujillo et al (2005). All taxa were positive for catalase and oxidase activity.…”

Section: Description Of Zhangella Mobilis Sp Novmentioning

confidence: 91%

Zhangella mobilis gen. nov., sp. nov., a new member of the family Hyphomicrobiaceae isolated from coastal seawater

Chen

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Zhangella mobilis gen. nov., sp. nov., a new member of the family Hyphomicrobiaceae isolated from coastal seawater A Gram-negative, pale-yellow, non-spore-forming bacterium, motile with single polar flagella, strain E6 T , was isolated from coastal seawater collected from Tianjin, China, and its taxonomic position was investigated using a polyphasic approach. Strain E6 T requires NaCl for growth and grows optimally at pH 8.2 and 36 6C and in the presence of 2.0 % (w/v) NaCl. It is positive for catalase and oxidase, and reduces nitrate to nitrite. The major fatty acids (.10 %) are C 18 : 1 v7c (64.71 %) and 11-methyl C 18 : 1 v7c (12.38 %), the ubiquinone system is Q-10 and the DNA G+C content is 53.1 mol%. Phylogenetic analyses based on 16S rRNA gene sequences demonstrated that strain E6 T represented a new lineage in the Alphaproteobacteria and is related to genera Cucumibacter, Devosia, Ochrobactrum and Ahrensia. Strain E6 T shows highest 16S rRNA gene sequence similarity (93.3 %) to Cucumibacter marinus CL-GR60 T and less than 92.2 % similarity to other relatives. It can be differentiated from its closest phylogenetic neighbours on the basis of several phenotypic features, including nitrate reduction, assimilation of D-glucose, L-arabinose, D-mannose, mannitol and maltose and major fatty acid composition. A polyphasic analysis supported the conclusion that strain E6 T represents a novel genus and species of the family Hyphomicrobiaceae, for which the name Zhangella mobilis gen. nov., sp. nov. is proposed. The type strain of Zhangella mobilis is E6 T (5CGMCC 1.7002 T 5JCM 15144 T ). In a survey of the ecology and bacterial diversity of coastal seawater of Tianjin, China, many bacterial strains were isolated from seawater samples. One of them, designated E6 T , was phylogenetically related to the genera Cucumibacter, Devosia and Ochrobactrum. The levels of 16S rRNA gene sequence similarity between E6 T and related strains of the above three genera were below 93.3 %. In this communication, we describe the isolation and polyphasic taxonomic study of strain E6 T . The familyFor isolation, serial dilutions of a seawater sample were spread onto solid low-organic seawater medium [LOSWM; containing (l 21 seawater) 1.0 g peptone, 0.5 g yeast extract and 15 g agar] and incubated at 30 u C for 10 days. Individual colonies were picked and cultured in LOSWM broth and stored at 220 uC in 20 % glycerol (v/v). Strain E6 T was obtained and it could also grow in marine broth 2216 (MB; Difco) and on marine agar 2216 (MA; Difco).Routine cultivation was conducted at 30 u C on MA. Flagellation was examined by tannin staining (Dong & Cai, 2001) and transmission electron microscopy (H600; Hitachi) after negative staining with 1 % (w/v) phosphotungstic acid. Morphological observations were carried out using scanning electron microscopy (Quanta 200; FEI).3These authors contributed equally to this paper.The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain E6 T is EU255260.A transmission electron micrograph and...

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Section: Description Of Zhangella Mobilis Sp Novmentioning

confidence: 91%

Zhangella mobilis gen. nov., sp. nov., a new member of the family Hyphomicrobiaceae isolated from coastal seawater

Chen

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…The genus Ochrobactrum was introduced by Holmes et al (1988) and, at the time of writing, the genus comprises the nine species Ochrobactrum anthropi (Holmes et al, 1988), O. intermedium (Velasco et al, 1998), O. tritici and O. grignonense (Lebuhn et al, 2000), O. gallinifaecis (Kämpfer et al, 2003b), O. lupini (Trujillo et al, 2005), O. oryzae (Tripathi et al, 2006), O. pseudintermedium (ZurdoPiñeiro et al, 2007) and O. cytisi (Teyssier et al, 2007).…”

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confidence: 99%

Ochrobactrum haematophilum sp. nov. and Ochrobactrum pseudogrignonense sp. nov., isolated from human clinical specimens

Kämpfer

Scholz

Huber³

et al. 2007

International Journal of Systematic and Evolutionary Microbiology

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Three Gram-negative, rod-shaped, non-spore-forming bacteria were isolated from clinical specimens between 1992 and 2000. On the basis of 16S rRNA gene sequence similarities, these strains (CCUG 30717T, CCUG 43892 and CCUG 38531T) were shown to belong to the Alphaproteobacteria, most closely related to Ochrobactrum grignonense (99.0 and 98.2 % similarity to the type strain). Chemotaxonomic data (major ubiquinone Q-10; major polyamines spermidine, sym-homospermidine and putrescine; major polar lipids phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol and phosphatidylcholine; major fatty acids C18 : 1 ω7c and C19 : 0 cyclo ω8c) supported the affiliation of the isolates to the genus Ochrobactrum. The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of the isolates from described Ochrobactrum species. Isolates CCUG 30717T and CCUG 43892 were closely related on the basis of DNA–DNA reassociation experiments and therefore represent one novel species, for which the name Ochrobactrum pseudogrignonense sp. nov. is proposed, with the type strain CCUG 30717T (=CIP 109451T). Isolate CCUG 38531T was different from these strains and also from other Ochrobactrum species. For this strain, the name Ochrobactrum haematophilum sp. nov. is proposed, with the type strain CCUG 38531T (=CIP 109452T).

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“…The genus Ochrobactrum currently comprises nine species, Ochrobactrum anthropi (Holmes et al, 1988), Ochrobactrum intermedium (Velasco et al, 1998), Ochrobactrum lupini (Trujillo et al, 2005), Ochrobactrum tritici and Ochrobactrum grignonense , Ochrobactrum gallinifaecis (Kämpfer et al, 2003), Ochrobactrum oryzae (Tripathi et al, 2006), Ochrobactrum pseudintermedium (Teyssier et al, 2007) and Ochrobactrum cytisi (Zurdo-Piñeiro et al, 2007). Only three species, O. anthropi, O. intermedium and O. pseudintermedium, have been reported to occur in clinical material (Alnor et al, 1994;Appelbaum & Campbell, 1980;Barson et al, 1987;Braun et al, 1996;Brivet et al, 1993;Cieslak et al, 1992Cieslak et al, , 1996Earhart et al, 1997;Greven & Nelson, 2001;Inoue et al, 1999;Jelveh & Cunha, 1999;Kettaneh et al, 2003;Mahmood et al, 2000;Perez-Blanco et al, 2005;Romero-Gomez et al, 2004;Vaidya et al, 2006;Wheen et al, 2002;Teyssier et al, 2007).…”

Section: Jmm Correspondencementioning

confidence: 99%

“…Only three species, O. anthropi, O. intermedium and O. pseudintermedium, have been reported to occur in clinical material (Alnor et al, 1994;Appelbaum & Campbell, 1980;Barson et al, 1987;Braun et al, 1996;Brivet et al, 1993;Cieslak et al, 1992Cieslak et al, , 1996Earhart et al, 1997;Greven & Nelson, 2001;Inoue et al, 1999;Jelveh & Cunha, 1999;Kettaneh et al, 2003;Mahmood et al, 2000;Perez-Blanco et al, 2005;Romero-Gomez et al, 2004;Vaidya et al, 2006;Wheen et al, 2002;Teyssier et al, 2007). O. anthropi was described by Holmes et al (1988) to accommodate oxidase-positive, Gram-negative, non-fermentative bacilli previously known as 'Achromobacter group Vd', occasionally infecting humans.…”

Section: Jmm Correspondencementioning

confidence: 99%

“…In 1999, O. intermedium was isolated from blood and a liver abscess in a patient after liver transplantation (Möller et al, 1999), and in 2005, a presumptive systemic O. intermedium infection in a patient with bladder cancer was reported (Apisarnthanarak et al, 2005). Recently a novel species, O. pseudintermedium, was described that was isolated from patients hospitalized in intensive-care units (Teyssier et al, 2007). The differentiation of Ochrobactrum species by physiological tests is quite difficult, because of their high phenotypic similarity (Table 1).…”

Section: Jmm Correspondencementioning

confidence: 99%

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Difficulty in the identification and differentiation of clinically relevant Ochrobactrum species

Kämpfer

Citron²,

Goldstein

et al. 2007

Journal of Medical Microbiology

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Ochrobactrum pseudintermedium sp. nov., a novel member of the family Brucellaceae, isolated from human clinical samples

Cited by 56 publications

References 26 publications

Zhangella mobilis gen. nov., sp. nov., a new member of the family Hyphomicrobiaceae isolated from coastal seawater

Zhangella mobilis gen. nov., sp. nov., a new member of the family Hyphomicrobiaceae isolated from coastal seawater

Ochrobactrum haematophilum sp. nov. and Ochrobactrum pseudogrignonense sp. nov., isolated from human clinical specimens

Difficulty in the identification and differentiation of clinically relevant Ochrobactrum species

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