Ocimum gratissimum enhances insulin sensitivity in male Wistar rats with dexamethasone-induced insulin resistance

Shittu, ST; Lasisi, Taye Jemilat; Shittu, Seyid Alli-Sisse; Adeyemi, A. A.; Adeoye, Tolulope; Alada, A R A

doi:10.1007/s40200-021-00850-y

Cited by 7 publications

(6 citation statements)

References 56 publications

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“…In line with the hyperglycemic impact of dexamethasone and an increase in insulin levels linked with dexamethasone therapy both in vivo and in vitro, Shittu et al [9] observed a significant rise in blood glucose levels in dexamethasone-treated rats. They stated that an adaptive reaction of the pancreatic islet to hyperglycemia results in elevated insulin levels.…”

Section: Discussionmentioning

confidence: 88%

“…The metabolic profile of rats treated with dexamethasone showed an increase in amino acids that are implicated in the suppression of protein synthesis and protein breakdown [9] . Exposure to a high dosage of dexamethasone leads to an increase in reactive oxygen species production that results in mitochondrial dysfunction, elevated cytosolic calcium, and irreversible oxidative modifications of cellular lipids, proteins, and DNA eventually causing cellular injury [12] .…”

Section: Discussionmentioning

confidence: 99%

“…➢ Group 2 [dexamethasone-treated group]: eight rats were treated with dexamethasone sodium phosphate [manufactured by Amriya Pharmaceuticals Industries, Alexandria, Egypt] at a dose of 1 mg/kg body weight via intraperitoneal injection daily for 2 weeks [9] . ➢ Group 3 [dexamethasone and garlic treated group]: eight rats were treated with dexamethasone sodium phosphate at a dose of 1 mg/kg body weight via intraperitoneal injection daily and treated with garlic water extract at a dose of 500mg/kg body weight via intraperitoneal injection daily for 2 weeks [6] .…”

Section: Methodsmentioning

confidence: 99%

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Study of the protective effect of garlic water extract on dexamethasone-induced hepatotoxicity and insulin resistance in adult male albino rats

Alkot

Naeem²,

Sayed³

et al. 2022

International Journal of Medical Arts

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Article informationBackground: The liver has an essential role in the metabolism and detoxification of various metabolites and drugs so it is liable to injury. Long-term use of dexamethasone may cause hepatic injury and insulin resistance. Garlic contains a variety of effective compounds that exhibit antioxidant, hypocholesterolemic, hypoglycemic, antithrombotic, antibiotic, anticancer, as well as hypotensive activities.Objective: To study the effect of garlic water extract on hepatic toxicity and insulin resistance induced by dexamethasone in adult male albino rats. Materials and Methods:Twenty-four adult male albino rats of a local strain were divided into three equal groups, group 1 [control group], group 2 [dexamethasone-treated group], and group 3 [dexamethasone and garlictreated group]. After two weeks from the onset of the experiment serum was isolated from blood samples for assessment of Alanine Transaminase [ALT], Aspartate Transaminase [AST], serum albumin, Alkaline Phosphatase [ALP], total bilirubin, fasting blood glucose [FBG], and Fasting insulin level. Homeostasis Model Assessment for Insulin Resistance [HOMA-IR] was then calculated. Samples of the liver were taken for histopathological studies.Results: There was a significant elevation in ALT, AST, ALP, and total bilirubin and a significant reduction in serum albumin in the dexamethasone-treated group when compared with the control group. ALT, AST, ALP, and total bilirubin significantly improved in the dexamethasone and garlic extracttreated group. Also, there was a significant elevation in FBS, insulin level, and HOMA-IR in the dexamethasone-treated group when compared with the control group. These parameters were significantly decreased in the dexamethasone and garlic extract-treated group when compared with the dexamethasone-only treated group. Conclusion:Garlic water extract showed a potential protective effect against dexamethasone-induced liver injury and insulin resistance.

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Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Study of the protective effect of garlic water extract on dexamethasone-induced hepatotoxicity and insulin resistance in adult male albino rats

Alkot

Naeem²,

Sayed³

et al. 2022

International Journal of Medical Arts

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“…The pancreatic tissues were fixed in 10% formaldehyde. 36,37 RSC Medicinal Chemistry Research Article…”

Section: Blood and Tissue Samplingmentioning

confidence: 99%

Development of novel thiazolidine-2,4-dione derivatives as PPAR-γ agonists through design, synthesis, computational docking, MD simulation, and comprehensive in vitro and in vivo evaluation

Gowdru Srinivasa,

B. C.,

Prabhu

et al. 2023

RSC Med. Chem.

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“…MD simulations were performed using the Desmond software [33,34] to investigate the interactions between the protein PPAR-γ and several ligand molecules (2b, 2g, 2h, and Pioglitazone). The simulations employed the optimized potentials for liquid simulations (OPLS)-2005 force eld and the simple point charged (TIP4P) water model for solvation.…”

Section: Molecular Dynamics Simulationsmentioning

confidence: 99%

Novel Thiazolidinedione Derivatives as effective agonists of Peroxisome Proliferator Activator Receptor-γ: A Comprehensive Study on Design, Synthesis, Molecular Docking, MD Simulations, and Antidiabetic Efficacy

Sriniv

Prabhu

Rani

et al. 2023

Preprint

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In the quest for potential anti-diabetic agents, a novel series of thiazolidinedione derivatives (2a-j)were synthesized and characterized by spectroscopic data. These newly developed derivatives were subjected to molecular docking studies, utilizing the binding pocket of the Peroxisome proliferator-activated receptor-gamma (PPAR-γ) agonist, to understand their binding interactions. To assess the stability of the ligand-PPAR-γ complex, molecular dynamics (MD) simulations were conducted for synthesized compounds. The cytotoxicity of the compounds was evaluated in C2C12 myoblasts using the MTT assay. Additionally, the compounds were tested on C2C12 cell lines to confirm their PPAR-γ transcription activity. Furthermore, the synthesized compounds underwent evaluation for their in vivo blood glucose-lowering activity using a dexamethasone-induced diabetic rat model. Significantly, compounds 2b, 2g, and 2h demonstrated potential as PPAR-γ agonists compared to the reference drug Pioglitazone. Data analysis revealed noteworthy blood glucose-lowering effects (hypoglycaemia) for compounds 2b, 2g, and 2h (110.04±5.42, 114.52±6.32, and 118.48±5.93, respectively), as well as for the reference drug Pioglitazone (153.93±4.61), when compared to the diabetic control. Moreover, compounds 2b, 2g, and 2h exhibited significant reductions in the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP), indicating the absence of liver damage.

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Ocimum gratissimum enhances insulin sensitivity in male Wistar rats with dexamethasone-induced insulin resistance

Cited by 7 publications

References 56 publications

Study of the protective effect of garlic water extract on dexamethasone-induced hepatotoxicity and insulin resistance in adult male albino rats

Study of the protective effect of garlic water extract on dexamethasone-induced hepatotoxicity and insulin resistance in adult male albino rats

Development of novel thiazolidine-2,4-dione derivatives as PPAR-γ agonists through design, synthesis, computational docking, MD simulation, and comprehensive in vitro and in vivo evaluation

Novel Thiazolidinedione Derivatives as effective agonists of Peroxisome Proliferator Activator Receptor-γ: A Comprehensive Study on Design, Synthesis, Molecular Docking, MD Simulations, and Antidiabetic Efficacy

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