1999
DOI: 10.1590/s0101-20611999000200019
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Ocorrência de fungos filamentosos termo-resistentes em polpa de tomate envasada assepticamente

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Cited by 14 publications
(16 citation statements)
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“…Further, appropriate decimal dilutions were performed and plated on PDA media plus tartaric acid solution (1/10, mass/volume) until pH 3.5 was reached and 0.1% (in mass) of a Rose Bengal solution (5/ 100 mass/volume) to inhibit the spread of other undesirable microbial organisms (Baglioni et al, 1999). The plates were wrapped in a plastic film to prevent drying.…”
Section: Spore Suspension Quantificationmentioning
confidence: 99%
“…Further, appropriate decimal dilutions were performed and plated on PDA media plus tartaric acid solution (1/10, mass/volume) until pH 3.5 was reached and 0.1% (in mass) of a Rose Bengal solution (5/ 100 mass/volume) to inhibit the spread of other undesirable microbial organisms (Baglioni et al, 1999). The plates were wrapped in a plastic film to prevent drying.…”
Section: Spore Suspension Quantificationmentioning
confidence: 99%
“…4). The calculation of F considered 10 N. fischeri ascospores per liter juice pack (Baglioni et al, 1999) and accepted only 1 in 10 3 infected packs (4 decimal reductions).…”
Section: Calculation Of Sterilization (F Value) For Apple Concentratementioning
confidence: 99%
“…Spoilage by heat-resistant fungi is a major problem for fruit-processing industries and has been documented in many countries (Baglioni, Gumerato, & Massaguer, 1999;Kotzekidou, 1997;Obeta & Ugwuanyi, 1995;Rajashekhara, Suresh, & Ethiraj, 1996;Suresh, Ethiraj, & Jayaram, 1996). Contamination of processed fruits by fungi originates in fields and orchards, where the fungi have been isolated (Tournas, 1994).…”
Section: Introductionmentioning
confidence: 99%
“…Além disso, optouse por esta metodologia, pois ela pode ser utilizada por todas as amostras, independente dos índices de sólidos solúveis das mesmas. Terminado o choque térmico das amostras líquidas, estas foram resfriadas em um banho de gelo e 10 mL de cada amostra foram plaqueados em profundidade (triplicata) em meio Ágar Batata Dextrose (pH 5,6; MERCK) (PDA) preparado com 3% de ágar e adicionado de 50 mg.L -1 de rosa de bengala, para evitar o espalhamento das colônias (BAGLIONI; GUMERATO;MASSAGUER, 1999). Após o choque térmico, a amostra da matéria-prima, que foi triturada juntamente com o meio de cultura PDA (como descrito anteriormente), teve seu volume total diretamente distribuído em placas de Petri.…”
Section: Isolamento Dos Fungos Termorresistentesunclassified
“…A fim de selecionar o isolado mais termorresistente, as suspensões de esporos preparadas (item 2.3) foram submetidas a diferentes choques térmicos, variando de 80 °C/20 minutos a 97 °C/15 minutos, se necessário (BAGLIONI; GUMERATO;MASSAGUER, 1999). A Tabela 1 mostra os diferentes choques térmicos aplicados, conforme necessidade.…”
Section: Aplicação Dos Choques Térmicos Para a Seleção Do Isolado Maiunclassified