Ocotillol Enhanced the Antitumor Activity of Doxorubicin via p53-Dependent Apoptosis

Wang, Hongbo; Yu, Pengfei; Bai, Jing; Zhang, Jianqiao; Kong, Liang; Fang-xi, Zhang; Du, Guangying; Pei, Shiqian; Zhang, Lixia; Jiang, Yongtao; Tian, Jingwei; Fu, Fenghua

doi:10.1155/2013/468537

Cited by 15 publications

(15 citation statements)

References 25 publications

(30 reference statements)

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“…Cell viability was measured using an MTT assay according to our previously published protocol 12 . Briefly, the cells were seeded in a 96-well plate and incubated for 24 h, then treated with vehicle control or Lx2-32c for 72 h. MTT solution (5 mg/mL) was added into each well and incubated for another 2 h. DMSO was added and the optical density was measured at 570 nm using a Molecular Devices SpectraMax M5 (Sunnyvale, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Lx2-32c, a novel semi-synthetic taxane, exerts antitumor activity against prostate cancer cells in vitro and in vivo

Lv¹,

Sun²,

Zhang³

et al. 2017

Acta Pharmaceutica Sinica B

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Tubulin has been shown to be an effective target for the development of cytotoxic agents against prostate cancer. Previously, we reported that Lx2-32c is an anti-tubulin agent with high binding affinity to tubulin. In this study, we investigated the potential of Lx2-32c to act as an effective cytotoxic agent in the treatment of prostate cancer. MTT assays showed that Lx2-32c was cytotoxic to all tested prostate cancer cell lines. The Lx2-32c-treated cells typically exhibited a rounded morphology associated with the onset of apoptosis, as evidenced by immunocytochemical staining. Human prostate cancer cell lines treated with Lx2-32c arrest in the G2/M phase of the cell cycle and the treatment is associated with an increased ratio of cells in the sub-G0/G1 phase as determined by flow cytometry. Furthermore, expression of the cleaved form of poly (ADP-ribose) polymerase in prostate cancer cell lines treated with Lx2-32c was shown by Western blotting assay. Xenograft implants of LNCaP and PC3-derived tumors in nude mice showed that Lx2-32c treatment significant inhibited tumor growth with effects equivalent to those of docetaxel. These findings demonstrate the potential of Lx2-32c as a candidate antitumor agent for the treatment of prostate cancer.

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Section: Methodsmentioning

confidence: 99%

Lx2-32c, a novel semi-synthetic taxane, exerts antitumor activity against prostate cancer cells in vitro and in vivo

Lv¹,

Sun²,

Zhang³

et al. 2017

Acta Pharmaceutica Sinica B

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“…6) Xenograft tumor model in nude mice The xenograft tumors model of human cervical cancer (Siha) is established using female balb/c nu mice as previously reported [17] . Briefly, the tumor is implanted at the dorsum after isolated from the donor animals.…”

Section: ) Lipid Peroxidation and Antioxidants Measurementmentioning

confidence: 99%

“…The tumors are measured every 3 days, and the relative tumor volume is calculated as previously described [17] . At the end of experiment, the animals are sacrificed, in which the tumor are separated and weighed.…”

Section: ) Lipid Peroxidation and Antioxidants Measurementmentioning

confidence: 99%

Ameliorative effect of ginsenoside RT-5 on CDDP-induced nephrotoxicity

Jiang

Qiu

et al. 2015

Wuhan Univ. J. Nat. Sci.

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The aim of this study is to explore whether RT-5, one novel active ginsenosides from Ginseng, has protective effects against cisplatin (CDDP)-induced nephrotoxicity in vivo. One model of acute renal failure induced by CDDP in rats and one model of xenograft tumors of human cervical cancer in nude mice are established. Four groups are assigned: control, CDDP, RT-5, CDDP plus RT-5, in which the RT-5 is administered via oral gavage 24 h before CDDP intraperitoneal injection. The significant increase in blood urea nitrogen and creatinine are induced by CDDP treatment at 6 mg/kg, which are attenuated by pre-treated with RT-5 at 10 mg/kg. RT-5 could ameliorate CDDP-induced morphological damages in kidney by PAS staining, and reduce tubular apoptosis evaluated by TUNEL staining. Pretreatment with RT-5 notably inhibits CDDP-induced oxidative stress in kidney tissues. Interestingly, RT-5 does not interfere with the in vivo anti-cancer effects of CDDP against the growth of xenograft tumors in nude mice. These data suggest that co-treatment RT-5 with CDDP might attenuate the following nephrotoxicity without inhibiting its anti-tumor efficiency, which could provide one novel strategy for cancer treatment in clinics.

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“…Total mRNA was prepared and RT-PCR was performed as previously described (18). Briefly, the cells were washed twice with D-Hank's solution after treatment and total RNA was extracted from the cultured cortical neurons using a TRIzol extraction kit.…”

Section: Reverse Transcription-polymerase Chain Reaction (Rt-pcr)mentioning

confidence: 99%

Angiotensin II protects cortical neurons against oxygen-glucose deprivation-induced injury in vitro

et al. 2013

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Abstract. Ischemic cerebrovascular disease is a common type of cerebrovascular disease and the leading cause of disability and mortality worldwide. Therefore, it is crucial to elucidate its pathogenesis and develop novel therapeutic strategies. This study was performed to investigate whether angiotensin (Ang) II exerts a protective effect against cerebral ischemia̸reperfusion (I/R) injury in vitro. The primary cultured neurons were prepared and an I/R model was established by incubation of cortical neurons with Na 2 S 2 O 4 , followed by culture in fresh medium. The protective effect of Ang II and its underlying mechanisms were investigated by morphology observation, MTT assay, flow cytometry analysis and reverse transcription-polymerase chain reaction (RT-PCR). The data demonstrated that Ang II significantly ameliorated the neuronal injury caused by oxygen-glucose deprivation. Furthermore, Ang II increased cell viability through inhibiting cell apoptosis. The RT-PCR results revealed that Ang II was able to reverse the increased bax mRNA and the decreased bcl2 mRNA expression. Of note, the protective activity of Ang II may be attenuated by co-treatment with Ang II type 2 (AT2) receptor blockade (PD123319), but not Ang II type 1 (AT1) receptor blockade (valsartan). These findings suggested that Ang II exerted a protective effect against neuronal injury induced by oxygen-glucose deprivation through decreasing cell apoptosis. Therefore, Ang II may be used as a potential therapeutic target in the future.

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Ocotillol Enhanced the Antitumor Activity of Doxorubicin via p53-Dependent Apoptosis

Cited by 15 publications

References 25 publications

Lx2-32c, a novel semi-synthetic taxane, exerts antitumor activity against prostate cancer cells in vitro and in vivo

Lx2-32c, a novel semi-synthetic taxane, exerts antitumor activity against prostate cancer cells in vitro and in vivo

Ameliorative effect of ginsenoside RT-5 on CDDP-induced nephrotoxicity

Angiotensin II protects cortical neurons against oxygen-glucose deprivation-induced injury in vitro

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