Oct-3 is a maternal factor required for the first mouse embryonic division

Rosner, Mitchell H.; Santo, Ronald J. De; Heinz, Andreas; Staudt, Louis M.

doi:10.1016/0092-8674(91)90265-z

Cited by 58 publications

(24 citation statements)

References 21 publications

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“…Similar experiments suggest a dual role for a maternally expressed POU protein, Oct-3, whose expression is restricted to the female germ line and to totipotent and pluripotent stem cells before gastrulation (31,38). Studies with antisense oligonucleotides have shown that Oct-3 function is required for the first cleavage in mouse embryos, suggesting a possible role for this protein in DNA replication (30).…”

mentioning

confidence: 78%

Sequential expression of multiple POU proteins during amphibian early development.

Hinkley¹,

Martin²,

Leibham³

et al. 1992

Mol. Cell. Biol.

101

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The octamer motif is a common cis-acting regulatory element that functions in the transcriptional control regions of diverse genes and in viral origins of replication. The , were present at low levels in oocytes and early embryos and were dramatically upregulated during early gastrulation. In contrast to the Oct-60 mRNA, translation of Oct-25 mRNA appeared to be developmentally regulated, since the corresponding protein was detected in embryos during gastrulation but not in oocytes or rapidly cleaving embryos. Transcripts from the third POU protein gene, Oct-91, were induced after the midblastula transition and reached their highest levels of accumulation during late gastrulation. The expression of all three genes decreased during late gastrulation and early neurulation. By analogy with other members of the POU-domain gene family, the products of these genes may play critical roles in the determination of cell fate and the regulation of cell proliferation.

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mentioning

confidence: 78%

Sequential expression of multiple POU proteins during amphibian early development.

Hinkley¹,

Martin²,

Leibham³

et al. 1992

Mol. Cell. Biol.

101

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“…The second argument for the specificity of antisense oligomers is that they can produce biological effects on cultured cells that are specific by the criterion that several different antisense oligomers produce the same effect, while control oligomers do not. In some of the most convincing studies, the effect has been reversed by replacing the depleted RNA species (3,4), demonstrating that the antisense oligomers do not produce detectable side effects in these assays. These results are encouraging, but it is difficult to extrapolate to the in vivo situation, since many genes that are not required for viability in culture may nevertheless be required for more subtle functions in vivo.…”

mentioning

confidence: 99%

Specificity of antisense oligonucleotides in vivo.

Woolf

Melton

Jennings

1992

Proc. Natl. Acad. Sci. U.S.A.

239

102

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Antisense oligonucleotides are widely used as inhibitors of gene expression in cultured cells and have been proposed as potential therapeutic agents, but it is not known to what extent they are specific for their intended target RNAs. Statistical considerations indicate that if oligonucleotides can form hybrids with mRNA molecules in vivo by means of short or imperfect regions of complementarity, then the specificity of oligonucleotides as antisense reagents will be greatly compromised. We have used Xenopus oocytes as a model system in which to investigate the potential specificity of antisense oligonucleotides in vivo. We injected perfect and partially matched antisense oligonucleotides into oocytes and measured the resulting degradation of the target RNA in each case. On the basis of the extent to which antisense oligonucleotides can cause cleavage of RNAs at imperfectly matched target sites, we conclude that in this system it is probably not possible to obtain specific cleavage of an intended target RNA without also causing at least the partial destruction of many nontargeted RNAs.

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“…For example, the polyomavirus (Py) on functions only in those mouse cell types that can activate its enhancer (6,12,58,74). In mammalian chromosomes, active genes are replicated early during S phase while quiescent genes are replicated late (82), and initiation of replication may require specific transcription factors (76) that bind DNA sites where replication begins (11).…”

mentioning

confidence: 99%

Specific transcription factors stimulate simian virus 40 and polyomavirus origins of DNA replication.

Guo¹,

DePamphilis²

1992

Mol. Cell. Biol.

102

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The origins of DNA replication (ori) in simian virus 40 (SV40) and polyomavirus (Py) contain an auxiliary component (aux-2) composed of multiple transcription factor binding sites. To determine whether this component stimulated replication by binding specific transcription factors, aux-2 was replaced by synthetic oligonucleotides that bound a single transcription factor. Spl and T-antigen (T-ag) sites, which exist in the natural SV40 aux-2 sequence, provided -75 and -20%, respectively, of aux-2 activity when transfected into monkey cells. In cell extracts, only T-ag sites were active. AP1 binding sites could replace completely either SV40 or Py aux-2. Mutations that eliminated API binding also eliminated AP1 stimulation of replication. Yeast GAL4 binding sites that strongly stimulated transcription in the presence of GAL4 proteins failed to stimulate SV40 DNA replication, although they did partially replace Py aux-2. Stimulation required the presence of proteins consisting of the GAL4 DNA binding domain fused to specific activation domains such as VP16 or c-Jun. These data demonstrate a clear role for transcription factors with specific activation domains in activating both SV40 and Py ori. However, no correlation was observed between the ability of specific proteins to stimulate promoter activity and their ability to stimulate origin activity. We propose that only transcription factors whose specific activation domains can interact with the T-ag initiation complex can stimulate SV40 and Py ori-core activity.Most, if not all, of the eukaryotic origins of DNA replication (on) characterized so far consist of two principal components: the core component that determines where replication begins in the chromosome and in which animal species replication occurs, and one or more auxiliary components that stimulate replication in certain cell types (28,30,96). on-core is the minimal cis-acting sequence required to initiate DNA replication under all conditions; it is analogous to a transcription promoter. on-auxiliary sequences generally consist of transcription factor binding sites that are dispensable under some conditions; they are analogous to transcription enhancers. The purpose of auxiliary components may be to regulate DNA replication by determining when initiation occurs. For example, the polyomavirus (Py) on functions only in those mouse cell types that can activate its enhancer (6,12,58,74). In mammalian chromosomes, active genes are replicated early during S phase while quiescent genes are replicated late (82), and initiation of replication may require specific transcription factors (76) that bind DNA sites where replication begins (11).To elucidate the function of on-auxiliary components in mammals, we turned our attention to the origins of DNA replication in simian virus 40 (SV40) and Py. These two closely related viral chromosomes replicate in the nuclei of mammalian cells and, with the exception of a single viral protein (large tumor antigen [T-ag]), rely entirely on the host to replicate their DNA (13,29)....

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Oct-3 is a maternal factor required for the first mouse embryonic division

Cited by 58 publications

References 21 publications

Sequential expression of multiple POU proteins during amphibian early development.

Sequential expression of multiple POU proteins during amphibian early development.

Specificity of antisense oligonucleotides in vivo.

Specific transcription factors stimulate simian virus 40 and polyomavirus origins of DNA replication.

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