OCT intensity and phase fluctuations correlated with activity-dependent neuronal calcium dynamics in the Drosophila CNS [Invited]

Tong, Minh; Hasan, Monirul; Lee, Sang Soo; Haque, Rezuanul; Kim, Dohyoung; Islam, Shahidul; Adams, Michael E.; Park, B Hyle

doi:10.1364/boe.8.000726

Cited by 12 publications

(7 citation statements)

References 55 publications

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“…Phase sensitivity ( Δ ) in OCT is intimately linked to SNR, motion (Δx) of the sample, and the spot size ('d') 42,43 as shown in the equation 1 below.…”

Section: Phase Sensitivitymentioning

confidence: 99%

High-speed adaptive optics line-scan OCT for cellular-resolution optoretinography

Pandiyan

Jiang

Bertelli

et al. 2020

Preprint

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Optoretinographythe non-invasive, optical imaging of light-induced functional activity in the retinastands to provide a critical biomarker for testing the safety and efficacy of new therapies as well as their rapid translation to the clinic. Optical phase change in response to light, as readily accessible in phaseresolved OCT, offers a path towards all-optical imaging of retinal function. However, typical human eye motion adversely affects phase stability and precludes the recording of fast light-induced retinal events.Here we introduce a high-speed line-scan spectral domain OCT with adaptive optics (AO), aimed at volumetric imaging and phase-resolved acquisition of retinal responses to light. By virtue of parallel acquisition of an entire retinal cross-section (B-scan) in a single high-speed camera frame, depth-resolved tomograms at speeds up to 16 kHz were achieved with high sensitivity and phase stability. To optimize spectral and spatial resolution, an anamorphic detection paradigm was introduced enabling improved light collection efficiency and signal roll-off compared to traditional methods. The benefits in speed, resolution and sensitivity were exemplified in imaging nanometer-millisecond scale light-induced optical path length changes in cone photoreceptor outer segments. With 660 nm stimuli, individual cone responses readily segregated into three clusters, corresponding to long, middle and short-wavelength cones. Recording such optoretinograms on spatial scales ranging from individual cones, to 100 µm-wide retinal patches offers a robust and sensitive biomarker for cone function in health and disease. Furthermore, incorporating this capability into an easy-to-use and ubiquitous diagnostic platform of OCT enables its widespread application to patient care and drug development.

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“…Phase sensitivity ( Δ ) in OCT is intimately linked to SNR, motion (Δx) of the sample, and the spot size ('d') 42,43 as shown in the equation 1 below.…”

Section: Phase Sensitivitymentioning

confidence: 99%

High-speed adaptive optics line-scan OCT for cellular-resolution optoretinography

Pandiyan

Jiang

Bertelli

et al. 2020

Preprint

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“…15,23,57 The fast temporal part of the fOCT signal might also involve neuronal membrane displacements (or deformations) during stimulation, which has been detected with phase-sensitive interferometric or low-coherence imaging techniques. [24][25][26][58][59][60][61] Typically, nanometer-scale displacements were detected on a millisecond time scale and were thought to be caused by the swelling and shrinkage of the nerve fiber 24,25,60 and neural cell bodies. 58,61 Akkin et al 25 reported that the nerve displacements accompanied fast light scattering changes.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, fOCT is a potentially attractive tool for detecting electrical activity in neural tissue in vivo. [18][19][20][21][22][23][24][25][26][27] For many studies (e.g., aimed at neuromodulation or functional tract tracing), neural stimulation methods are desirable. Electrical stimulation has been a long-standing tool for neurostimulation, 28,29 but it suffers from spread of electrical current, often leading to unwanted activation of additional brain circuits and resulting side effects.…”

Section: Introductionmentioning

confidence: 99%

INS-fOCT: a label-free, all-optical method for simultaneously manipulating and mapping brain function

et al. 2020

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Significance: Current approaches to stimulating and recording from the brain have combined electrical or optogenetic stimulation with recording approaches, such as two-photon, electrophysiology (EP), and optical intrinsic signal imaging (OISI). However, we lack a label-free, all-optical approach with high spatial and temporal resolution. Aim: To develop a label-free, all-optical method that simultaneously manipulates and images brain function using pulsed near-infrared light (INS) and functional optical coherence tomography (fOCT), respectively. Approach: We built a coregistered INS, fOCT, and OISI system. OISI and EP recordings were employed to validate the fOCT signals. Results: The fOCT signal was reliable and regional, and the area of fOCT signal corresponded with the INS-activated region. The fOCT signal was in synchrony with the INS onset time with a delay of ∼30 ms. The magnitude of fOCT signal exhibited a linear correlation with the INS radiant exposure. The significant correlation between the fOCT signal and INS was further supported by OISI and EP recordings. Conclusions: The proposed fiber-based, all-optical INS-fOCT method allows simultaneous stimulation and mapping without the risk of interchannel cross-talk and the requirement of contrast injection and viral transfection and offers a deep penetration depth and high resolution.

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“…Despite the popularity of fluorescence imaging, label-free optical detection of action potential (AP) is highly desirable as it eliminates the need to add exogenous chemical or genetic biosensors with inevitable issues associated with photo-bleaching, photo-toxicity, low SNR and the potential of interfering with normal physiological functions. Endogenous optical changes due to action potential propagation can manifest as changes in scattering [14, 31- Reported label-free AP studies using optical techniques have been conducted in drosophila CNS [42], giant squid axon [43,44], lobster [45], and rat-nerve bundle [46,47] models which typically require averaging of multiple optical signals due to poor signal to noise ratio. In recent years, a number of label free optical techniques [14,[31][32][33][34][35][36][37][38][42][43][44][45] based on phase sensitive interferometry have demonstrated detection of neural activity.…”

Section: Introductionmentioning

confidence: 99%

Label-free optical detection of action potential in mammalian neurons

Batabyal

Satpathy

Bui

et al. 2017

Biomed. Opt. Express

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Abstract:We describe an optical technique for label-free detection of the action potential in cultured mammalian neurons. Induced morphological changes due to action potential propagation in neurons are optically interrogated with a phase sensitive interferometric technique. Optical recordings composed of signal pulses mirror the electrical spike train activity of individual neurons in a network. The optical pulses are transient nanoscale oscillatory changes in the optical path length of varying peak magnitude and temporal width. Exogenous application of glutamate to cortical neuronal cultures produced coincident increase in the electrical and optical activity; both were blocked by application of a Nachannel blocker, Tetrodotoxin. The observed transient change in optical path length in a single optical pulse is primarily due to physical fluctuations of the neuronal cell membrane mediated by a yet unknown electromechanical transduction phenomenon. Our analysis suggests a traveling surface wave in the neuronal cell membrane is responsible for the measured optical signal pulses. Corrected: 25 July 2017 11. M. E. Spira and A. Hai, "Multi-electrode array technologies for neuroscience and cardiology," Nat. Nanotechnol. 8(2), 83-94 (2013). 12. X. Li, W. Zhou, M. Liu, and Q. Luo, "Synchronized spontaneous spikes on multi-electrode array show development of cultured neuronal network," Conf.

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OCT intensity and phase fluctuations correlated with activity-dependent neuronal calcium dynamics in the Drosophila CNS [Invited]

Cited by 12 publications

References 55 publications

High-speed adaptive optics line-scan OCT for cellular-resolution optoretinography

High-speed adaptive optics line-scan OCT for cellular-resolution optoretinography

INS-fOCT: a label-free, all-optical method for simultaneously manipulating and mapping brain function

Label-free optical detection of action potential in mammalian neurons

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