OCT4 and SOX2 are reliable markers in detecting stem cells in odontogenic lesions

Banerjee, Abhishek; Kamath, Venkatesh V; Sundaram, Lavanya; Krishnamurthy, Shruthi

doi:10.4103/0975-8844.181920

Cited by 8 publications

(5 citation statements)

References 2 publications

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“…[44,45] On the other hand, a recent study by Pagella et al,2020 [46] has documented that SOX2 was highly expressed by AB. Moreover, Banerjee et al, 2016 [47] stated a total absence of SOX2 in all their studied AB cases. These conflicting results could be explained on the basis of a recent study on the genetic alterations of AB [48] which found multiple gene mutations only in European patients except the Turkish ones.…”

Section: Discussionmentioning

confidence: 85%

Prognostic significance of SOX2 and GPC3 in Ameloblastoma and its malignant counterpart (Ameloblastic Carcinoma)

Hasan

Nagdy

Ibrahim

2021

JST

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Background: Ameloblastoma is a common benign aggressive odontogenic tumor with a tendency for high recurrence rate. Ameloblastic Carcinoma is the malignant counterpart of Ameloblastoma. However, they are usually difficult to be distinguished from one another. Therefore, using Immunohistochemical markers might be beneficial for diagnosing them accurately.Objective: Evaluation of SOX2 and GPC3 expressions as well as evaluating their roles in the tumorigenesis and the biological behavior of Ameloblastoma and Ameloblastic Carcinoma.Methods: Tissue samples are composed of 34 archived histopathologically confirmed cases of (19 Conventional Ameloblastomas, and 15 Ameloblatic Carcinomas). Sections were subjected to Immunohistochemical staining according to a standard protocol by applying antibodies to SOX2, and GPC3.Results: SOX2 and GPC3 expressions in recurrent Ameloblastoma were significantly higher than non- recurrent cases. Ameloblastic Carcinoma showed the highest immune-reactivity to SOX2 and GPC3 compared to the Conventional Ameloblastoma. Desmoplastic Ameloblastoma showed the highest scores of SOX2 and GPC3 compared to the other subtypes.Conclusions: SOX2 and GPC3 can be used as a panel for diagnosing the aggressive and the malignant odontogenic tumors accurately. Desmoplastic Ameloblastoma behaves more aggressively than other Conventional Ameloblastoma subtypes.

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Section: Discussionmentioning

confidence: 85%

Prognostic significance of SOX2 and GPC3 in Ameloblastoma and its malignant counterpart (Ameloblastic Carcinoma)

Hasan

Nagdy

Ibrahim

2021

JST

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“…It is generally a well-agreed fact that the embryo is a potential source of pluripotent progenitor cells, which divides and gives rise to different types of cells, specialized in forming different tissues. [ 7 ] There are four important cell-specific factors, which are expressed in the cells with pluripotent capacity, i.e., octamer-binding transcription factor 4 (OCT4), sex-determining region Y (SRY)-box2 (SOX2), NANOG and c-Myc. [ 7 ]…”

Section: Discussionmentioning

confidence: 99%

“…[ 7 ] There are four important cell-specific factors, which are expressed in the cells with pluripotent capacity, i.e., octamer-binding transcription factor 4 (OCT4), sex-determining region Y (SRY)-box2 (SOX2), NANOG and c-Myc. [ 7 ]…”

Section: Discussionmentioning

confidence: 99%

“…They found that negative expression of both SOX2 and OCT4 was observed in ameloblastoma, whereas odontogenic keratocyst showed a positive SOX2 expression and a negative OCT4 expression, which was in accordance with the present study. [ 7 ]…”

Section: Discussionmentioning

confidence: 99%

“…Embryonic stem cell (ESC) markers such as OCT4 and SOX2 are capable of identifying these stem cells expressed during the early stages of tooth development (dental papilla and dental lamina cells). [ 7 8 ]…”

Section: Introductionmentioning

confidence: 99%

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Immunohistochemical expression of SOX2 in OKC and ameloblastoma: A comparative study

Pereira,

Shetty,

Punjabi

et al. 2023

Journal of Oral and Maxillofacial Pathology

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Introduction: Odontogenic, non-inflammatory maxillofacial cysts and tumours vary greatly in their ability to grow and cause local tissue destruction. Despite their common embryologic origin, the biologic mechanisms responsible for this diverse array of clinical behaviour are largely unknown. Unfortunately, even with accurate tissue diagnosis and appropriate surgical management, these tumours have relatively high recurrence rates. While this may be related to surgical technique, it may also be due to intrinsic tumour biology. SOX2 is differentially expressed in odontogenic cysts and tumours, which has an impact over patient prognosis. This could be related to their diverse cells of origin or stages of histogenesis. SOX2 is expressed in OKC and ameloblastoma, and in this study, we look forward to find altered levels and intensity of SOX2 in the above-mentioned lesions. Aim and Objectives: To profile the expression of SOX2 in odontogenic keratocyst (OKC) and ameloblastomaTo compare the intensity of these lesions, analyse their intrinsic feature and predict their recurrence Material and Methods: Histopathologically diagnosed cases of OKC and ameloblastoma will be selected (n = 40). Paraffin-embedded, formalin-fixed sections of these lesions will be stained for SOX2 marker using a standard immunohistochemical technique. Positive control will be taken as oral squamous cell carcinoma and negative control will be taken as normal oral mucosa. Results: A comparison between the stained cell types in odontogenic keratocyst and ameloblastoma revealed statistically significant differences. The immunoreactivity scores of SOX2 were analysed in both groups. The results indicated that 45% of OKC cases exhibited strongly positive reactivity, while 65% of ameloblastoma cases were negative. Statistical analysis demonstrated highly significant differences in the frequency of SOX2 expression between the two groups, with a higher frequency of negative expression in ameloblastoma. Conclusion: Stem cell markers have been observed in these lesions, suggesting the acquisition of stem-like properties by tumour cells, which can affect patient prognosis. Specifically, the marker SOX2 shows differential expression in odontogenic cysts and tumours. High expression of SOX2 in OKC indicates the presence of stem cells with significant self-renewal and proliferative properties, potentially signifying neoplastic behaviour. In contrast, weak or absent expression of SOX2 in ameloblastoma suggests different molecular pathways involved in its neoplastic behaviour.

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Oct-4 and CD44 in epithelial stem cells like of benign odontogenic lesions

Monroy

Santos

Lopes

et al. 2018

Histochem Cell Biol

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Benign epithelial odontogenic lesions are great clinical importance entities that develop in the jaws from the tissues that form teeth. It has been shown that benign and malignant tumors are present in a large number of tumor stem cells, which has great implications in the development of these lesions. Oct-4 and CD44 have been demonstrated as important markers for tumoral stem cells. The aim of this study was investigate the presence of stem cell markers Oct-4 and CD44 in benign epithelial odontogenic lesions. Twenty odontogenic keratocysts (OKC), 20 ameloblastomas (AMB) of the solid/multicystic type and 20 adenomatoid odontogenic tumors (AOT) were retrospectively analyzed for immunohistochemical detection of Oct-4 and CD44 in their epithelial component. All cases were positive for the two markers, with the majority exhibiting a high expression. Analysis of the expression of Oct-4 revealed no statistically significant differences (p = 0.406) between the lesions studied. Regarding CD44, there was a significant difference between the cases of AMB and AOT in relation with OKC, with the latter presenting a greater labelling (p = 0.034). No statistically significant correlation between Oct-4 and CD44 was observed in the lesions. In our findings, the presence of stem cell-like phenotype at various sites of the epithelial component of the odontogenic lesions was identified, suggesting its possible participation in histogenesis and differentiation without, however, exerting influence on the aggressiveness of the lesions.

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OCT4 and SOX2 are reliable markers in detecting stem cells in odontogenic lesions

Cited by 8 publications

References 2 publications

Prognostic significance of SOX2 and GPC3 in Ameloblastoma and its malignant counterpart (Ameloblastic Carcinoma)

Prognostic significance of SOX2 and GPC3 in Ameloblastoma and its malignant counterpart (Ameloblastic Carcinoma)

Immunohistochemical expression of SOX2 in OKC and ameloblastoma: A comparative study

Oct-4 and CD44 in epithelial stem cells like of benign odontogenic lesions

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