Oct4 transcription factor in conjunction with valproic acid accelerates myelin repair in demyelinated optic chiasm in mice

Dehghan, Samaneh; Hesaraki, Mahdi; Soleimani, Masoud; Mirnajafi‐Zadeh, Javad; Fathollahi, Yaghoub; Javan, Mohammad

doi:10.1016/j.neuroscience.2016.01.028

Cited by 31 publications

(14 citation statements)

References 43 publications

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“…Lentiviral plasmid and virus production was performed according to a protocol described previously (40,41). Briefly, the cDNAs of Msx1 and Msx2 were amplified by PCR and cloned into third generation lentiviral expression vectors.…”

Section: Plasmid Constructions Virus Production and Msx1 And Msx2 Gmentioning

confidence: 99%

Msh homeobox 1 (Msx1)- and Msx2-overexpressing bone marrow-derived mesenchymal stem cells resemble blastema cells and enhance regeneration in mice

Taghiyar

Hesaraki

Sayahpour

et al. 2017

Journal of Biological Chemistry

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Amputation of the proximal region in mammals is not followed by regeneration because blastema cells (BCs) and expression of regenerative genes, such as Msh homeobox () genes, are absent in this animal group. The lack of BCs and positional information in other cells is therefore the main obstacle to therapeutic approaches for limb regeneration. Hence, this study aimed to create blastema-like cells (BlCs) by overexpressing and genes in mouse bone marrow-derived mesenchymal stem cells (mBMSCs) to regenerate a proximally amputated digit tip. We transduced mBMSCs with and genes and compared osteogenic activity and expression levels of several -regulated genes (, , and keratin 14 ()) in BlC groups, including MSX1, MSX2, and MSX1/2 (in a 1:1 ratio) with those in mBMSCs and BCs and following injection into the amputation site. We found that gene overexpression increased expression of specific blastemal markers and enhanced the proliferation rate and osteogenesis of BlCs compared with mBMSCs and BCs via activation of and Histological analyses indicated full regrowth of digit tips in the-overexpressing groups, particularly in MSX1/2, through endochondral ossification 6 weeks post-injection. In contrast, mBMSCs and BCs formed abnormal bone and nail. Full digit tip was regenerated only in the MSX1/2 group and was related to boosted , and gene expression and to limb-patterning properties resulting from and overexpression. We propose that -transduced cells that can regenerate epithelial and mesenchymal tissues may potentially be utilized in limb regeneration.

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Section: Plasmid Constructions Virus Production and Msx1 And Msx2 Gmentioning

confidence: 99%

Msh homeobox 1 (Msx1)- and Msx2-overexpressing bone marrow-derived mesenchymal stem cells resemble blastema cells and enhance regeneration in mice

Taghiyar

Hesaraki

Sayahpour

et al. 2017

Journal of Biological Chemistry

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“…In a very recent work, we showed that intracerebroventricular (i.c.v.) injection of Yamanaka factor Oct4 in conjunction with VPA enhanced myelin regeneration in demyelinated mouse optic chiasm (Dehghan et al , ).…”

Section: Introductionmentioning

confidence: 99%

In vivo conversion of astrocytes to myelinating cells by miR-302/367 and valproate to enhance myelin repair

Ghasemi-Kasman

Zare

Baharvand

et al. 2017

J Tissue Eng Regen Med

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Enhancement of repair potential for degenerative brain diseases has been a research priority during recent years. Considering recent advancements in the field of direct transdifferentiation, conversion of astrocytes as a prominent component of glial scars to the progenitor cells that contribute to the repair mechanisms seems interesting. Recently, we have reported miR-302/367-mediated in vivo conversion of astrocytes into neuroblasts and neurons. In the current study, we used miR-302/367 and valproate (VPA) to show the possibility of conversion of astrocytes to oligodendrocyte progenitor cells and myelinating cells in a cuprizone (CPZ)-induced model of demyelination. Evaluation of behavioural impairment following CPZ and consequent to the treatments showed functional recovery from impairments. Enhanced remyelination was detected by luxol fast blue staining and immunostaining against two mature myelin markers, myelin basic protein and proteolipid protein. Tracing of transduced cells with green fluorescent protein showed their contribution toward generation of new myelinating cells. These findings have suggested that in vivo specific targeting of astrocytes for forced expression of the miR-302/367 cluster and VPA administration may increase the brain's potential for repairing myelin insults by the generation of oligodendroglia from astrocytes.This finding may open a new avenue for enhancement of brain repair in neurodegenerative diseases such as multiple sclerosis.

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“…AR signaling [3], Wnt signaling [4] and MAPK signaling [5,6] were reported to be the most important pathways in PCa. Recently, emerging studies indicated non-coding RNAs, including miRNAs [7], lncRNAs [8,9] and circRNAs [10], were also participated in the regulation of PCa related pathways. For instance, miR-183 promoted PSA synthesis in PCa [11], PCAT19 induced PCa growth and metastasis by interacting with hRNPAB to promote cell cycle regulators expression [12].…”

Section: Introductionmentioning

confidence: 99%

FAM64A Promotes Prostate Cancer Growth In Vivo and In Vitro

Cui

et al. 2020

Preprint

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Background Prostate cancer (PCa) is the most common type of human cancer in males. However, the mechanisms underlying PCa tumorigenesis remained unclear.Methods The present study evaluated the expression levels of FAM64A in PCa by using 5 public datasets, including GSE8511, GSE45016, GSE55945, GSE38241 and GSE17951. Then, in vivo and in vitro assays were conducted to detect the biological functions of FAM64A in PCa. Microarray and bioinformatic analysis were carried out to detect the downstream targets and pathways regulated by FAM64A.Results In this study, we for first time demonstrate FAM64A as a biomarker for PCa. FAM64A was found to be overexpressed in PCa compared to normal samples. Higher FAM64A expression were found in Gleason score (GS) ≥ 8 PCa compared to GS < 8 PCa samples, in N1 staging compared to N1 staging PCa samples, and T3/4 staging compared to T1 staging PCa. Moreover, higher FAM64A expression was correlated to shorter survival time in PCa. Knockdown of FAM64A significantly suppressed PCa cell proliferation and colony formation, however, induced PCa apoptosis in vivo and in vitro. Bioinformatics analysis combined with microarray analysis revealed FAM64A played crucial roles in regulating multiple cancer related pathways, including cell-matrix adhesion and cAMP signaling pathway. Conclusions These results showed FAM64A could serve as a novel biomarker for PCa and will be helpful to understand the underlying FAM64A -related molecular mechanisms in the progression of PCa.

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Oct4 transcription factor in conjunction with valproic acid accelerates myelin repair in demyelinated optic chiasm in mice

Cited by 31 publications

References 43 publications

Msh homeobox 1 (Msx1)- and Msx2-overexpressing bone marrow-derived mesenchymal stem cells resemble blastema cells and enhance regeneration in mice

Msh homeobox 1 (Msx1)- and Msx2-overexpressing bone marrow-derived mesenchymal stem cells resemble blastema cells and enhance regeneration in mice

In vivo conversion of astrocytes to myelinating cells by miR-302/367 and valproate to enhance myelin repair

FAM64A Promotes Prostate Cancer Growth In Vivo and In Vitro

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