OCT4B1 Regulates the Cellular Stress Response of Human Dental Pulp Cells with Inflammation

Abstract

Introduction. Infection and apoptosis are combined triggers for inflammation in dental tissues. Octamer-binding transcription factor 4-B1 (OCT4B1), a novel spliced variant of OCT4 family, could respond to the cellular stress and possess antiapoptotic property. However, its specific role in dental pulpitis remains unknown. Methods. To investigate the effect of OCT4B1 on inflammation of dental pulp cells (DPCs), its expression in inflamed dental pulp tissues and DPCs was examined by in situ hybridization, real-time PCR, and FISH assay. OCT4B1 overexpressed DPCs model was established, confirmed by western blot and immunofluorescence staining, and then stimulated with Lipopolysaccharide (LPS). Apoptotic rate was determined by Hoechst/PI staining and FACS. Cell survival rate was calculated by CCK8 assay. Results. In situ hybridization, real-time PCR, and FISH assay revealed that OCT4B1 was extensively expressed in inflamed dental pulp tissues and DPCs with LPS stimulation. Western blot and immunofluorescence staining showed the expression of OCT4B1 and OCT4B increased after OCT4B1 transfection. Hoechst/PI staining and FACS demonstrated that less red/blue fluorescence was detected and apoptotic percentage decreased (3.45%) after transfection. CCK8 demonstrated that the survival rate of pCDH-OCT4B1-flag cells increased. Conclusions. OCT4B1 plays an essential role in inflammation and apoptosis of DPCs. OCT4B might operate synergistically with OCT4B1 to reduce apoptosis.

IL‐33 guides osteogenesis and increases proliferation and pluripotency marker expression in dental stem cells

“…Previous studies indicated that OCT4B might be involved in cellular stress response and antiapoptotic activity in cancer cells . Importantly, OCT4B may indirectly be increased in dental pulp tissues and dental pulp cells exposed to proinflammatory factors, where it has been suggested that OCT4B represents a antiapoptotic mediator and subsequently may be the important factor in dental regeneration . Our results indicate that IL‐33 increases expression of OCT4B mRNA expression in both PDLSCs and DPSCs.…”

Survival/Adaptation of Bone Marrow-Derived Mesenchymal Stem Cells After Long-Term Starvation Through Selective Processes

“…It has been demonstrated that OCT4B isoforms are associated with a response to oxidative and genotoxic stress, as well as with an antiapoptotic activity [51][52][53]. Here, OCT4B isoforms were shown to be faintly expressed during starvation and increased during the proliferative phase poststarvation.…”

Role of Lipopolysaccharide, Derived from Various Bacterial Species, in Pulpitis—A Systematic Review

“…LPS stimulation also upregulates octamer-binding transcription factor 4-B1 (OCT4B1) leading to overexpression of OCT4B [ 8 ]. Both particles protect hDPCs from apoptosis [ 9 ]. Silencing OCT4B1 changes the expression of 38 microRNAs [ 8 ].…”