Octacalcium Phosphate for Bone Tissue Engineering: Synthesis, Modification, and In Vitro Biocompatibility Assessment

Teterina, A. Yu.; Smirnov, Igor; Фадеева, И. С.; Фадеев, Р. С.; Smirnova, Polina V.; Minaychev, Vladislav V.; Kobyakova, M. I.; Fedotov, A. Yu.; Баринов, С. М.; Komlev, V. S.

doi:10.3390/ijms222312747

Cited by 15 publications

(10 citation statements)

References 50 publications

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“…Octacalcium phosphate (OCP, Ca 8 (HPO 4 ) 2 (PO 4 ) 4 •5H 2 O) has attracted immense attention in biomaterials research as a novel hard-tissue repair material [1]. Numerous studies report in vitro [2][3][4][5][6][7] and in vivo [8][9][10][11][12][13][14][15][16][17][18] biological characterisations and clinical tests of OCPbased biomaterials [19][20][21][22][23]. Composite materials composed of OCP and collagen exhibited excellent biological properties, which make them a good substitute for autogenous bone.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Octacalcium Phosphate Containing Glutarate Ions with a High Incorporation Fraction

et al. 2022

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Octacalcium phosphate (OCP) has received considerable attention in the field of ceramic biomaterials as an advanced functional material. It exhibits a layered structure composed of apatitic and hydrated layers and can incorporate various dicarboxylate ions into the hydrated layer. Saturated dicarboxylic acids (HOOC(CH2)nCOOH) with an odd number of methylene groups (–CH2–) exhibit lower incorporation fractions than those with an even number of methylene groups, possibly owing to a compositional dependence on the synthetic method. In this study, calcium carbonate, phosphoric acid, and various amounts of glutaric acid were used to produce glutarate-ion-incorporated OCP by a wet chemical method, which is different from the conventional synthetic strategy. While utilising 1–20 mmol of glutaric acid during synthesis did not produce the desired product, using 25 mmol of glutaric acid resulted in the formation of single-phase glutarate-ion-incorporated OCP with a Ca/P molar ratio of 1.57 and a 90% incorporation fraction of glutarate ions. This glutarate-ion-incorporation fraction is significantly higher than that reported in the previous studies (35%). Thus, the synthetic procedure proposed herein was able to produce single-phase OCP containing glutarate ions with a high incorporation fraction. Our findings can contribute to development of novel functional ceramic biomaterials in the future.

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Section: Introductionmentioning

confidence: 99%

Synthesis of Octacalcium Phosphate Containing Glutarate Ions with a High Incorporation Fraction

et al. 2022

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“…The co-culture continued for an additional 72 h. DCPD, OCP, and HAp powders were pre-sterilized with an ethanol solution at a concentration of 75% using the specified technique [19].…”

Section: Cell Culturementioning

confidence: 99%

“…Interest in such CPs is justified by several studies demonstrating their greater biological activity compared to crystalline hydroxyapatite due to greater solubility and the ability to undergo phase transformation in the body into native bone tissue hydroxyapatite. In our opinion, the use of CPs synthesized under physiological conditions can significantly increase the biocompatibility of CP biomaterials [19][20][21].…”

Section: Introductionmentioning

confidence: 95%

Low-Temperature Calcium Phosphate Ceramics Can Modulate Monocytes and Macrophages Inflammatory Response In Vitro

Minaychev,

Smirnova,

Kobyakova

et al. 2024

Biomedicines

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Creating bioactive materials for bone tissue regeneration and augmentation remains a pertinent challenge. One of the most promising and rapidly advancing approaches involves the use of low-temperature ceramics that closely mimic the natural composition of the extracellular matrix of native bone tissue, such as Hydroxyapatite (HAp) and its phase precursors (Dicalcium Phosphate Dihydrate—DCPD, Octacalcium Phosphate—OCP, etc.). However, despite significant scientific interest, the current knowledge and understanding remain limited regarding the impact of these ceramics not only on reparative histogenesis processes but also on the immunostimulation and initiation of local aseptic inflammation leading to material rejection. Using the stable cell models of monocyte-like (THP-1ATRA) and macrophage-like (THP-1PMA) cells under the conditions of LPS-induced model inflammation in vitro, the influence of DCPD, OCP, and HAp on cell viability, ROS and intracellular NO production, phagocytosis, and the secretion of pro-inflammatory cytokines was assessed. The results demonstrate that all investigated ceramic particles exhibit biological activity toward human macrophage and monocyte cells in vitro, potentially providing conditions necessary for bone tissue restoration/regeneration in the peri-implant environment in vivo. Among the studied ceramics, DCPD appears to be the most preferable for implantation in patients with latent inflammation or unpredictable immune status, as this ceramic had the most favorable overall impact on the investigated cellular models.

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“…To control nonspecific staining, cells were incubated with 10 µg/mL cytochalasin D for 30 min in a CO 2 incubator, then 1 mg/mL pHrodo Green E. coli was added, and the incubation was continued for another 2 h. Fluorescence was measured using a BD Accuri C6 flow cytometer (BD Bioscience, Franklin Lakes, NJ, USA). Phagocytic activity was characterized based on the percentage of fluorescent cells (phagocytic index) and the average fluorescence intensity per cell (phagocytic number) in fluorescent cells [64,65]. Monocytes cultured in emulsion-free medium were used as a negative control.…”

Section: Phagocytosis Assaymentioning

confidence: 99%

Improving the Stability and Effectiveness of Immunotropic Squalene Nanoemulsion by Adding Turpentine Oil

Krasnova,

Minaychev,

Akatov

et al. 2023

Biomolecules

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Turpentine oil, owing to the presence of 7–50 terpenes, has analgesic, anti-inflammatory, immunomodulatory, antibacterial, anticoagulant, antioxidant, and antitumor properties, which are important for medical emulsion preparation. The addition of turpentine oil to squalene emulsions can increase their effectiveness, thereby reducing the concentration of expensive and possibly deficient squalene, and increasing its stability and shelf life. In this study, squalene emulsions were obtained by adding various concentrations of turpentine oil via high-pressure homogenization, and the safety and effectiveness of the obtained emulsions were studied in vitro and in vivo. All emulsions showed high safety profiles, regardless of the concentration of turpentine oil used. However, these emulsions exhibited dose-dependent effects in terms of both efficiency and storage stability, and the squalene emulsion with 1.0% turpentine oil had the most pronounced adjuvant and cytokine-stimulating activity as well as the most pronounced stability indicators when stored at room temperature. Thus, it can be concluded that the squalene emulsion with 1% turpentine oil is a stable, monomodal, and reliably safe ultradispersed emulsion and may have pleiotropic effects with pronounced immunopotentiating properties.

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Octacalcium Phosphate for Bone Tissue Engineering: Synthesis, Modification, and In Vitro Biocompatibility Assessment

Cited by 15 publications

References 50 publications

Synthesis of Octacalcium Phosphate Containing Glutarate Ions with a High Incorporation Fraction

Synthesis of Octacalcium Phosphate Containing Glutarate Ions with a High Incorporation Fraction

Low-Temperature Calcium Phosphate Ceramics Can Modulate Monocytes and Macrophages Inflammatory Response In Vitro

Improving the Stability and Effectiveness of Immunotropic Squalene Nanoemulsion by Adding Turpentine Oil

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