Octamer-binding protein 4 affects the cell biology and phenotypic transition of lung cancer cells involving β-catenin/E-cadherin complex degradation

Chen, Zhongshu; Ling, Dong‐Jin; Yang-de, Zhang; Feng, Jian‐Xiong; Zhang, Xue‐Yu; Shi, Tian‐Sheng

doi:10.3892/mmr.2014.2992

Cited by 9 publications

(6 citation statements)

References 35 publications

(35 reference statements)

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“…For instance, Chen et al showed that OCT4 has the capacity to induce lung cancer cell metastasis via promoting epithelial–mesenchymal transition (EMT). 13 Knockout of OCT4 decreased cell proliferation rate by modulating EMT processes in hepatocellular carcinoma cell lines. 8 Moreover, OCT4 overexpression promoted tumorigenesis while inhibiting cell apoptosis through regulation of miR-125b/BAK1 pathways in cervical cancer.…”

Section: Discussionmentioning

confidence: 98%

OCT4, SOX2, and NAN OG positive expression correlates with poor differentiation, advanced disease stages, and worse overall survival in HER2<sup>+</sup> breast cancer patients

Yang¹,

Zhang²,

Yang³

2018

OTT

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ObjectiveThis study aimed to evaluate the correlations of expression of OCT4, SOX2, and NANOG with clinicopathological features and overall survival (OS) in human epidermal growth factor receptor 2-positive (HER2+) breast cancer (BC) patients.MethodsOne hundred and thirty-four surgical HER2+ BC patients who received doxorubicin and cyclophosphamide followed by paclitaxel and trastuzumab adjuvant therapy were enrolled in this study. Immunofluorescence assay was used to detect OCT4, SOX2, and NANOG expressions. The median follow-up duration was 104 months, and the last follow-up date was December 31, 2017.ResultsThe expressions of OCT4 (P=0.001), SOX2 (P=0.003), and NANOG (P=0.005) were higher in tumor tissues compared with paired adjacent tissues. OCT4 positive expression was associated with poor pathological differentiation (P=0.028), larger tumor size (P=0.022), advanced N stage (P<0.001), and higher TNM stage (P<0.001). SOX2 positive expression was correlated with poor pathological differentiation (P=0.005), larger tumor size (P=0.013), and increased T stage (P=0.024). NANOG positive expression was associated with poor pathological differentiation (P=0.028), higher N stage (P=0.001), and elevated TNM stage (P=0.001). Kaplan–Meier curves disclosed that OCT4 (P=0.001) and NANOG (P=0.001) positive expressions were associated with worse OS, while SOX2 (P=0.058) positive expression was only numerically correlated with poor OS, but without statistical significance. Further analyses revealed that co-expression of these three biomarkers disclosed even better predictive value for shorter OS.ConclusionOCT4, SOX2, and NANOG positive expressions correlate with poor differentiation and advanced disease stage, and OCT4 and NANOG present with predictive values for poor OS in HER2+ BC patients.

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Section: Discussionmentioning

confidence: 98%

OCT4, SOX2, and NAN OG positive expression correlates with poor differentiation, advanced disease stages, and worse overall survival in HER2<sup>+</sup> breast cancer patients

Yang¹,

Zhang²,

Yang³

2018

OTT

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“…Chen et al showed that Oct4 increased the invasiveness of lung cancer cells, and induced mesenchymal markers such as vimentin and N-cadherin. Oct4 also regulated degradation of the β-catenin/E-cadherin complex [18]. On the other hand, Hu et al demonstrated that silencing Oct4 promoted the invasiveness and spread of breast cancer cell line MCF-7 by inducing EMT.…”

Section: Discussionmentioning

confidence: 99%

Expression of embryonal stem cell transcription factors in breast cancer: Oct4 as an indicator for poor clinical outcome and tamoxifen resistance

Gwak

Kim

et al. 2017

Oncotarget

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The transcription factors of embryonic stem cells, such as Oct4, Sox2, Nanog, Bmi1, and Klf4, are known to be associated with stemness, epithelial–mesenchymal transition and aggressive tumor behavior. This study was designed to evaluate the clinicopathological significance of their expression in breast cancer. Immunohistochemistry for Oct4, Sox2, Nanog, Bmi1, and Klf4 was performed in 319 cases of invasive breast cancer. The relationship between the expression of these markers and clinicopathologic features of the tumors, including breast cancer stem cell phenotype and epithelial–mesenchymal transition marker expression, and their prognostic value in breast cancer, were analyzed. Expression of Oct4 and Sox2 was commonly associated with high histologic grade and high Ki-67 index in the whole group and in the hormone receptor-positive subgroup. On the other hand, expression of Nanog, Bmi1, and Klf4 was inversely correlated with aggressive features of the breast cancer. Oct4 expression was associated with ALDH1 expression but not with epithelial–mesenchymal transition marker expression. In survival analysis, Oct4 expression was independently associated with poor prognosis in the whole group and in the hormone receptor-positive subgroup, but not in hormone receptor-negative subgroup. Particularly, Oct4 expression was associated with poor clinical outcome in patients with hormone receptor-positive breast cancer treated with tamoxifen. Our results indicate that Oct4 expression is associated with aggressive features, ALDH1 expression, tamoxifen resistance and poor clinical outcomes in hormone receptor-positive breast cancer, and thus may be useful as a predictive and prognostic marker in this subgroup of breast cancer.

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“…EMT is associated with tumor metastasis ( 35 , 36 ). EMT promotes cancer cell migration and invasion and has a crucial function in the invasion and metastasis of epithelial cancer specifically ( 35 , 36 ). EMT is associated with the downregulation of epithelial markers (including E-cadherin and β-catenin) and upregulation of interstitial markers (including N-cadherin and fibronectin).…”

Section: Discussionmentioning

confidence: 99%

Shikonin induces mitochondria-mediated apoptosis and attenuates epithelial-mesenchymal transition in cisplatin-resistant human ovarian cancer cells

et al. 2018

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Cisplatin-based chemotherapy often results in the development of chemoresistance when used to treat ovarian cancer, which is difficult to overcome. The present study investigated the cytotoxic and anti-migratory effects of shikonin, a naphthoquinone compound, on cisplatin-resistant human ovarian cancer A2780 cells (A2780-CR). Shikonin had a potent dose-dependent cytotoxic effect on A2780-CR cells, with 9 µM shikonin treatment reducing A2780-CR cell viability by 50%, validate using an MTT assay. Shikonin induced apoptosis, as evidenced by the increased number of apoptotic bodies, following staining with Hoechst 33342, and terminal deoxynucleotidyl cell transferase dUTP nick end labeling-positive cells following treatment. Flow cytometry and fluorescent microscope imaging, following JC-1 staining, revealed that shikonin increased mitochondrial membrane depolarization. Also it altered the levels of apoptosis-associated proteins, leading to diminished expression of B cell lymphoma-2 (Bcl-2), enhanced expression of Bcl-associated X, and cleavage of caspase-9 and −3, as revealed using western blot analysis. Shikonin activated mitogen-activated protein kinases; while treatment with specific inhibitors of these kinases attenuated the decline in cell viability induced by shikonin treatment. In addition, the cell migration assay and western blot analysis indicated that shikonin decreased the migratory capacity of A2780-CR cells via the upregulation of epithelial-cadherin and downregulation of neural-cadherin. Taken together, the results of the present study indicated that shikonin induces mitochondria-mediated apoptosis and attenuates the epithelial-mesenchymal transition in A2780-CR cells.

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Octamer-binding protein 4 affects the cell biology and phenotypic transition of lung cancer cells involving β-catenin/E-cadherin complex degradation

Cited by 9 publications

References 35 publications

OCT4, SOX2, and NAN OG positive expression correlates with poor differentiation, advanced disease stages, and worse overall survival in HER2<sup>+</sup> breast cancer patients

OCT4, SOX2, and NAN OG positive expression correlates with poor differentiation, advanced disease stages, and worse overall survival in HER2<sup>+</sup> breast cancer patients

Expression of embryonal stem cell transcription factors in breast cancer: Oct4 as an indicator for poor clinical outcome and tamoxifen resistance

Shikonin induces mitochondria-mediated apoptosis and attenuates epithelial-mesenchymal transition in cisplatin-resistant human ovarian cancer cells

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